1887

Abstract

Studies of the prevalence of complex species amongst cystic fibrosis (CF) patients in different geographical regions, and the association between cross-infection and putative transmissibility markers, will further our understanding of these organisms and help to address infection-control issues. In this study, complex isolates from CF patients in different regions of Europe were analysed. Isolates were examined for complex species and putative transmissibility markers [cable pilin subunit gene () and the epidemic strain marker (BCESM)]. Sporadic and cross-infective strains were identified by random amplification of polymorphic DNA (RAPD). In total, 79 % of patients were infected with (genomovar III), 18 % with (genomovar II) and less than 5 % of patients with (genomovar I), (genomovar IV) or (genomovar V). The and BCESM transmissibility markers were only detected in strains of . The BCESM was a more sensitive marker for transmissible strains than , although sporadic strains containing the BCESM, but lacking , were also observed. Furthermore, clusters of cross-infection with transmissibility marker-negative strains of were identified. In conclusion, was the greatest cause of cross-infection, and the most widely distributed complex species, within these CF populations. However, cross-infection was not exclusive to and and the BCESM were not absolute markers for transmissible , or other complex strains. It is therefore suggested that CF centres cohort patients based on the presence or absence of complex infection and not on the basis of transmissibility marker-positive as previously suggested.

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2004-07-01
2020-03-29
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References

  1. Agodi A., Mahenthiralingam E., Barchitta M., Giannino V., Sciacca A., Stefani S. 2001; Burkholderia cepacia complex infection in Italian patients with cystic fibrosis: prevalence, epidemiology, and genomovar status. J Clin Microbiol 39:2891–2896 [CrossRef]
    [Google Scholar]
  2. Biddick R., Spilker T., Martin A., LiPuma J. J. 2003; Evidence of transmission of Burkholderia cepacia , Burkholderia multivorans and Burkholderia dolosa among persons with cystic fibrosis. FEMS Microbiol Lett 228:57–62 [CrossRef]
    [Google Scholar]
  3. Chen J. S., Witzmann K. A., Spilker T., Fink R. J., LiPuma J. J. 2001; Endemicity and inter-city spread of Burkholderia cepacia genomovar III in cystic fibrosis. J Pediatr 139:643–649 [CrossRef]
    [Google Scholar]
  4. Clode F. E., Kaufmann M. E., Malnick H., Pitt T. L. 2000; Distribution of genes encoding putative transmissibility factors among epidemic and nonepidemic strains of Burkholderia cepacia from cystic fibrosis patients in the United Kingdom. J Clin Microbiol 38:1763–1766
    [Google Scholar]
  5. Drevinek P., Cinek O., Melter J., Langsadl L., Navesnakova Y., Vavrova V. 2003; Genomovar distribution of the Burkholderia cepacia complex differs significantly between Czech and Slovak patients with cystic fibrosis. J Med Microbiol 52:603–604 [CrossRef]
    [Google Scholar]
  6. Govan J. R. W., Brown P. H., Maddison J., Doherty C. J., Nelson J. W., Dodd M., Greening A. P., Webb A. K. 1993; Evidence for transmission of Pseudomonas cepacia by social contact in cystic fibrosis. Lancet 342:15–19 [CrossRef]
    [Google Scholar]
  7. Haworth C. S., Dodd M. E., Doherty C., Super M., Hambleton G., Vandamme P., Govan J. R. W., Webb A. K. 1997; The morbidity and mortality associated with two epidemic strains of Burkholderia cepacia with genomovar III status in cystic fibrosis patients. Pediatr Pulmonol Suppl 14290
    [Google Scholar]
  8. Lessie T. G., Hendrickson W., Manning B. D., Devereux R. 1996; Genomic complexity and plasticity of Burkholderia cepacia . FEMS Microbiol Lett 144:117–128 [CrossRef]
    [Google Scholar]
  9. LiPuma J. J., Dulaney B. J., McMenamin J. D., Whitby P. W., Stull T. L., Coenye T., Vandamme P. 1999; Development of rRNA-based PCR assays for identification of Burkholderia cepacia complex isolates recovered from cystic fibrosis patients. J Clin Microbiol 37:3167–3170
    [Google Scholar]
  10. LiPuma J. J., Spilker T., Gill L. H., Campbell P. W. III, Liu L., Mahenthiralingam E. 2001; Disproportionate distribution of Burkholderia cepacia complex species and transmissibility markers in cystic fibrosis. Am J Respir Crit Care Med 164:92–96 [CrossRef]
    [Google Scholar]
  11. LiPuma J. J., Spilker T., Coenye T., Gonzalez C. F. 2002; An epidemic Burkholderia cepacia complex strain identified in soil. Lancet 359:2002–2003 [CrossRef]
    [Google Scholar]
  12. Mahenthiralingam E., Campbell M. E., Henry D. A., Speert D. P. 1996; Epidemiology of Burkholderia cepacia infection in patients with cystic fibrosis: analysis by randomly amplified polymorphic DNA fingerprinting. J Clin Microbiol 34:2914–2920
    [Google Scholar]
  13. Mahenthiralingam E., Simpson D. A., Speert D. P. 1997; Identification and characterisation of a novel DNA marker associated with epidemic Burkholderia cepacia strains recovered from patients with cystic fibrosis. J Clin Microbiol 35:808–816
    [Google Scholar]
  14. Mahenthiralingam E., Bischof J., Byrne S. K., Radomski C., Davies J. E., Av-Gay Y., Vandamme P. 2000; DNA-based diagnostic approaches for identification of Burkholderia cepacia complex, Burkholderia vietnamiensis , Burkholderia multivorans , Burkholderia stabilis , and Burkholderia cepacia genomovars I and III. J Clin Microbiol 38:3165–3173
    [Google Scholar]
  15. Mahenthiralingam E., Vandamme P., Campbell M. E. & 7 other authors; 2001; Infection with Burkholderia cepacia complex genomovars in patients with cystic fibrosis: virulent transmissible strains of genomovar III can replace Burkholderia multivorans . Clin Infect Dis 33:1469–1475 [CrossRef]
    [Google Scholar]
  16. Mahenthiralingam E., Baldwin A., Vandamme P. 2002; Burkholderia cepacia complex infection in patients with cystic fibrosis. J Med Microbiol 51:533–538
    [Google Scholar]
  17. McDowell A., Mahenthiralingam E., Moore J. E. & 8 other authors; 2001; PCR-based detection and identification of Burkholderia cepacia complex pathogens in sputum from cystic fibrosis patients. J Clin Microbiol 39:4247–4255 [CrossRef]
    [Google Scholar]
  18. Sajjan U. S., Sun L., Goldstein R., Forstner J. F. 1995; Cable (Cbl) type II pili of cystic fibrosis-associated Burkholderia ( Pseudomonas ) cepacia : nucleotide sequence of the cblA major subunit pilin gene and novel morphology of the assembled appendage fibres. J Bacteriol 177:1030–1038
    [Google Scholar]
  19. Sajjan U., Liu L., Lu A., Spilker T., Forstner J., LiPuma J. J. 2002; Lack of cable pili expression by cblA -containing Burkholderia cepacia complex. Microbiology 148:3477–3484
    [Google Scholar]
  20. Segonds C., Heulin T., Marty N., Chabanon G. 1999; Differentiation of Burkholderia species by PCR-restriction fragment length polymorphism analysis of the 16S rRNA gene and application to cystic fibrosis isolates. J Clin Microbiol 37:2201–2208
    [Google Scholar]
  21. Speert D. P., Henry D., Vandamme P., Corey M., Mahenthiralingam E. 2002; Epidemiology of Burkholderia cepacia complex in patients with cystic fibrosis, Canada. Emerg Infect Dis 8:181–187 [CrossRef]
    [Google Scholar]
  22. Vandamme P., Holmes B., Vancanneyt M. & 8 other authors; 1997; Occurrence of multiple genomovars of Burkholderia cepacia in cystic fibrosis patients and proposal of Burkholderia multivorans sp.nov. Int J Syst Bacteriol 47:1188–1200 [CrossRef]
    [Google Scholar]
  23. Vandamme P., Mahenthiralingam E., Holmes B., Coenye T., Hoste B., De Vos P., Henry D., Speert D. P. 2000; Identification and population structure of Burkholderia stabilis sp.nov. (formerly Burkholderia cepacia genomovar IV. J Clin Microbiol 38:1042–1047
    [Google Scholar]
  24. Whiteford M. L., Wilkinson J. D., McColl J. H., Conlon F. M., Michie J. R., Evans T. J., Paton J. Y. 1995; Outcome of Burkholderia ( Pseudomonas ) cepacia colonisation in children with cystic fibrosis following a hospital outbreak. Thorax 50:1194–1198 [CrossRef]
    [Google Scholar]
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