1887

Abstract

Studies of the prevalence of complex species amongst cystic fibrosis (CF) patients in different geographical regions, and the association between cross-infection and putative transmissibility markers, will further our understanding of these organisms and help to address infection-control issues. In this study, complex isolates from CF patients in different regions of Europe were analysed. Isolates were examined for complex species and putative transmissibility markers [cable pilin subunit gene () and the epidemic strain marker (BCESM)]. Sporadic and cross-infective strains were identified by random amplification of polymorphic DNA (RAPD). In total, 79 % of patients were infected with (genomovar III), 18 % with (genomovar II) and less than 5 % of patients with (genomovar I), (genomovar IV) or (genomovar V). The and BCESM transmissibility markers were only detected in strains of . The BCESM was a more sensitive marker for transmissible strains than , although sporadic strains containing the BCESM, but lacking , were also observed. Furthermore, clusters of cross-infection with transmissibility marker-negative strains of were identified. In conclusion, was the greatest cause of cross-infection, and the most widely distributed complex species, within these CF populations. However, cross-infection was not exclusive to and and the BCESM were not absolute markers for transmissible , or other complex strains. It is therefore suggested that CF centres cohort patients based on the presence or absence of complex infection and not on the basis of transmissibility marker-positive as previously suggested.

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2004-07-01
2019-11-18
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