1887

Abstract

has frequently been isolated from human dental plaque, and oral spread via saliva is thought to be one of its principal modes of transmission. Among other innate defence systems human saliva contains peroxidase enzymes and lysozyme. The sensitivity of to physiological concentrations of lactoperoxidase and its salivary substrate thiocyanate, and different amounts of hydrogen peroxide (HO) was investigated in buffer and in human whole saliva. The effect of lysozyme was also studied in saliva. All tested strains, ATCC 43504 and five clinical isolates, were efficiently inhibited by the peroxidase system with high concentrations of HO in buffer. The inhibition was stronger at lower pH. However, in human saliva these high concentrations of HO generated less hypothiocyanite, the antibacterial product of the peroxidase system and the effects of the peroxidase system were weaker. Physiological concentration of lysozyme was not bacteriocidal against , nor did it enhance the effect of the peroxidase system in saliva. Thus, further studies are needed to enhance the efficacy of peroxidase systems in human saliva to make it more beneficial not only against dental but also against gastric pathogens.

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2004-09-01
2019-11-15
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