%0 Journal Article %A Seegmüller, Irene %A Eschenbach, Ursula %A Kamereck, Klaus %A Miethke, Thomas %T Sensitivity of the BacT/ALERT FA-medium for detection of Pseudomonas aeruginosa in pre-incubated blood cultures and its temperature-dependence %D 2004 %J Journal of Medical Microbiology, %V 53 %N 9 %P 869-874 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.45533-0 %I Microbiology Society, %X The BacT/ALERT FA-medium was evaluated to detect Pseudomonas aeruginosa in pre-incubated blood samples. As published previously its predecessor the BacT/ALERT FAN-medium failed to detect P. aeruginosa in delayed entry samples. It is now reported that FA-medium tolerates a longer pre-incubation period at 36 °C, i.e. 8 h, before detection of P. aeruginosa fails in experimentally inoculated blood cultures. In clinical blood samples the frequency of false-negative results concerning P. aeruginosa was reduced from 46.9 % (FAN-medium) to 9.1 % (FA-medium). If media inoculated with P. aeruginosa are pre-incubated at room temperature for 24 h, false-negative results are not observed. Various micro-organisms (Haemophilus influenzae, Streptococcus pneumoniae, Enterobacteriaceae, Staphylococcus aureus, Enterococcus faecalis, Candida glabrata) were recognized after pre-incubation at room temperature with similar sensitivity compared to pre-incubation at 36 °C. It is concluded that FA-medium detects P. aeruginosa in delayed entry samples with increased sensitivity and pre-incubation at room temperature is superior to pre-incubation at 36 °C. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.45533-0