The BacT/ALERT FA-medium was evaluated to detect in pre-incubated blood samples. As published previously its predecessor the BacT/ALERT FAN-medium failed to detect in delayed entry samples. It is now reported that FA-medium tolerates a longer pre-incubation period at 36 °C, i.e. 8 h, before detection of fails in experimentally inoculated blood cultures. In clinical blood samples the frequency of false-negative results concerning was reduced from 46.9 % (FAN-medium) to 9.1 % (FA-medium). If media inoculated with are pre-incubated at room temperature for 24 h, false-negative results are not observed. Various micro-organisms (, , , , , ) were recognized after pre-incubation at room temperature with similar sensitivity compared to pre-incubation at 36 °C. It is concluded that FA-medium detects in delayed entry samples with increased sensitivity and pre-incubation at room temperature is superior to pre-incubation at 36 °C.


Article metrics loading...

Loading full text...

Full text loading...



  1. Chapin, K. & Lauderdale, T. L. ( 1996;). Comparison of Bactec 9240 and Difco ESP blood culture systems for detection of organisms from vials whose entry was delayed. J Clin Microbiol 34, 543–549.
    [Google Scholar]
  2. Dunne, W. M., Jr, Nolte, F. S. & Wilson, M. L. ( 1997;). Cumitech 1B - Blood Cultures III, pp. 1–21. Edited by J. A. Hindler. Washington, DC: American Society for Microbiology.
  3. Gatermann, S., Podschun, R., Schmidt, H., Wittke, J.-W., Naber, K., Sietzen, W. & Straube, E. ( 1997;). Harnwegsinfektionen, pp. 1–35. Stuttgart: Gustav Fischer.
  4. Klaerner, H. G., Eschenbach, U., Kamereck, K., Lehn, N., Wagner, H. & Miethke, T. ( 2000;). Failure of an automated blood culture system to detect nonfermentative gram-negative bacteria. J Clin Microbiol 38, 1036–1041.
    [Google Scholar]
  5. Miller, J. M. & Holmes, H. T. ( 1995;). Specimen collection, transport and storage. In Manual of Clinical Microbiology, 6th edn, pp. 19–32. Edited by P. R. Murray, E. J. Baron, M. A. Pfaller, F. C. Tenover & R. H. Yolken. Washington, DC: American Society for Microbiology.
  6. Seifert, H., Shah, P., Ullmann, U. & 13 other authors ( 1997;). Sepsis-Blutkulturdiagnostik, pp. 1–44. Stuttgart: Gustav Fischer.
  7. Thomson, R. B. & Miller, J. M. ( 2003;). Specimen collection, transport and processing: bacteriology. In Manual of Clinical Microbiology, 8th edn, pp. 286–330. Edited by P. R. Murray, E. J. Baron, J. H. Jorgensen, M. A. Pfaller & R. H. Yolken. Washington, DC: American Society for Microbiology.
  8. Whimbey, E., Wong, B., Kiehn, T. E. & Armstrong, D. ( 1984;). Clinical correlations of serial quantitative blood cultures determined by lysis-centrifugation in patients with persistent septicemia. J Clin Microbiol 19, 766–771.
    [Google Scholar]

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error