1887

Abstract

Two hundred and fifty nine isolates of and related species were examined for the production of heat-stable enterotoxin ( stable toxin; YST) as well as for the prevalence of enterotoxin genes, viz. , and . Under the conventional conditions used for the production of enterotoxin, i.e. in tryptic soy broth (TSB) supplemented with yeast extract at 28 °C for 48 h, 77.7 % of clinical isolates and 62.3 % of swine isolates showed enterotoxigenicity in infant mice. All isolates that produced enterotoxin at 28 °C also showed enterotoxic activity at 37 °C after 48 h incubation under an alkaline pH of 7.5, the pH present in the ileum. All and isolates were negative for enterotoxin production. All clinical isolates and 96.3 % of isolates from swine hybridized with a probe for , which indicated that the gene was most prevalent in biotype 1A strains. None of the isolates showed hybridization with oligonucleotide probes for or . The study indicated that YST-b was the major contributor to diarrhoea produced by biotype 1A strains of .

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2004-11-01
2019-11-15
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