1887

Abstract

is one of the common pathogens associated with corneal infection, particularly in contact lens-related keratitis events. The pathogenesis of in keratitis is attributed to the production of virulence factors under certain environmental conditions. The aim of this study was to determine differences in the virulence factors of isolated from contact lens- and non-contact lens-related keratitis. Associations were assessed between type III secretion toxin-encoding genes, protease profiles, biofilm formation, serotypes and antibiotic-resistance patterns among 27 non-contact lens- and 28 contact lens-related keratitis isolates from Australia. Strains with a / genotype and a type I protease profile predominated in the non-contact lens-related keratitis isolates, whereas the / and a type II protease profile was associated with contact lens-related isolates (<0.05). A strong biofilm formation phenotype was found to be associated with the possession of the gene, and serotypes E, I and C. The gene was strongly associated with serotypes G, A and B, while was associated with serotypes E and C. Six out of fifty-five (11 %) clinical isolates were non-susceptible (intermediate-resistant or resistant) to ofloxacin and moxifloxacin. All resistant isolates were from non-contact lens-related keratitis. The results suggest that isolates from different infection origins may have different characteristics. A better understanding of these differences may lead to further development of evidence-based clinical guidelines for the management of keratitis.

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2008-12-01
2019-10-18
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