1887

Abstract

The species complex consists of two species, and , which cause systemic infections in both immunocompromised and immunocompetent patients. Both species have a bipolar mating system, with mating type () being predominant in clinical and environmental isolates. The strains of the species complex have been divided into eight major molecular types, which show differences in epidemiology, biology and pathogenicity. In this study, two PCR-RFLP analyses, based on the and genes, which are both located at the locus, were developed for simultaneous identification of the molecular and mating types of isolates of the species complex. The molecular and mating types of all 144 cryptococcal isolates, including rare subtypes, were successfully determined by both PCR-RFLP approaches. Pattern analysis of the AD hybrids revealed that the serotype A allele in strains of AD derived from genotype VNB, whereas the serotype A allele among strains of AD and AD derived from molecular type VNI.

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2008-12-01
2019-11-22
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