1887

Abstract

Since 1999, the European Antimicrobial Resistance Surveillance System (EARSS) has monitored the rise in infection due to a number of organisms, including meticillin-resistant (MRSA). The EARSS reported that MRSA infections within intensive care units account for 25–50 % of infections in many central and southern European countries, these included France, Spain, Great Britain, Malta, Greece and Italy. Each country has defined epidemic MRSA (EMRSA) strains; however, the method of spread of these strains from one country to another is unknown. In this current study, DNA profiles of 473 isolates of MRSA collected from the UK and Malta were determined by PFGE. Analysis of the data showed that two countries separated by a large geographical distance had a similar DNA profile pattern. Additionally it was demonstrated that strains of EMRSA normally found in the UK were also found in the Maltese cohort (EMRSA 15 and 16). A distinct DNA profile was found in the Maltese cohort, which may be a local EMRSA, and accounted for 14.4 % of all Maltese isolates. The appearance of the same MRSA and EMRSA profiles in two separate countries suggests that MRSA can be transferred out of their country of origin and potentially establish in a new locality or country.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.2008/003509-0
2008-11-01
2020-10-01
Loading full text...

Full text loading...

/deliver/fulltext/jmm/57/11/1394.html?itemId=/content/journal/jmm/10.1099/jmm.0.2008/003509-0&mimeType=html&fmt=ahah

References

  1. Aires de Sousa M., Sanches I. S., Ferro M. L., Vaz M. J., Saraiva Z., Tendeiro T., Serra J., de Lencastre H. 1998; Intercontinental spread of a multidrug-resistant methicillin-resistance Staphylococcus aureus clone. J Clin Microbiol 36:2590–2596
    [Google Scholar]
  2. Amorim M. L., Faria N. A., Oliveira D. C., Vasconcelos C., Cabeda J. C., Mendes A. C., Calado E., Castro A. P., Ramos M. H. other authors 2007; Changes in the clone nature and antibiotic resistance profiles of methicilin-resistance Staphylococcus aureus isolates associated with spread of the EMRSA-15 clone in tertiary care Portuguese hospital. J Clin Microbiol 45:2881–2888 [CrossRef]
    [Google Scholar]
  3. Bannerman T. L., Hancock G. A., Tenover F. C., Miller J. M. 1995; Pulse-field gel electrophoresis as a replacement for bacteriophage typing of Staphylococcus aureus . J Clin Microbiol 33:551–555
    [Google Scholar]
  4. BSAC 2004 BSAC Disc Diffusion Method for Antimicrobial Susceptibility Testing , version 3 Birmingham: British Society for Antimicrobial Chemotherapy; http://www.bsac.org.uk/_db/_documents/jan_2004_website_version_3.pdf
    [Google Scholar]
  5. da Silva Coimbra M. V., Silve-Carvalho M. C., Wisplinghoff H., Hall G. O., Tallent S., Wallace S., Edmond M. B., Figueiredo A. M., Wenzal R. P. 2003; Clonal spread of methicillin-resistant S taphylococcus aureus in a large geographic area of the United States. J Hosp Infect 53:103–110 [CrossRef]
    [Google Scholar]
  6. EARSS 2007 EARSS Annual Report 2006 Bilthoven: European Antimicrobial Resistance Surveillance System; http://www.rivm.nl/earss/Images/EARSS%202006%20Def_tcm61-44176.pdf
    [Google Scholar]
  7. Heym B., Le Moal M., Armand-Lefevre L., Nicolas-Chanoine M.-H. 2002; Multilocus sequence typing (MLST) shows that the “Iberian” clone of methicillin-resistant Staphylococcus aureus has spread to France and acquired reduced susceptibility to teicoplanin. J Antimicrob Chemother 50:323–329 [CrossRef]
    [Google Scholar]
  8. HPA 2000 Surveillance of Hospital-Acquired Bacteraemia in English Hospitals 1997–2002 , Nosocomial Infection National Surveillance Service and the Public Health Laboratory Service joint report London: Health Protection Agency (formerly the Public Health Laboratory Service); http://www.hpa.org.uk/web/HPAwebFile/HPAweb_C/1194947379958
    [Google Scholar]
  9. Hubert S. K., Mohammed J. M., Fridkin S. K., Gaynes R. P., McGowan J. E. Jr, Tenover F. C. 1999; Glycopeptide-intermediate Staphylococcus aureus : evaluation of a novel screening method and results of a survey of selected U.S. Hospitals. J Clin Microbiol 37:3590–3593
    [Google Scholar]
  10. Melter O., Urbášková P., Jakubů V., Macková B., Žemličková H. 2006; Emergence of EMRSA-15 clone in hospitals throughout the Czech Republic. Euro Surveill 11:E060803–6
    [Google Scholar]
  11. Montesinos I., Delgado T., Riverol D., Salido E., Miguel M. A., Jimenez A., Sierra A. 2006; Changes in the epidemiology of methicillin-resistant Staphylococcus aureus associated with the emergence of EMRSA-16 at a university hospital. J Hosp Infect 64:257–263 [CrossRef]
    [Google Scholar]
  12. Murchan S., Kaufmann M. E., Deplano A., de Ryck R., Struelens M., Zinn C. E., Fussing V., Salmenlinna S., Vuopio-Varkila J. other authors 2003; Harmonisation of pulse-field gel electrophoresis protocols for epidemiological typing of strains of methicillin-resistant Staphylococcus aureus: a single approach developed by consensus in 10 European laboratories and its application for tracing the spread of related strains. J Clin Microbiol 41:1574–1585 [CrossRef]
    [Google Scholar]
  13. Tenover F. C., Arbeit R. D., Goering R. V., Mickelsen P. A., Murray B. E., Persing D. H., Swaminathan B. 1995; Interpreting chromosomal DNA restriction patterns produced by pulse-field gel electrophoresis: criteria for bacterial strain typing. J Clin Microbiol 33:2233–2239
    [Google Scholar]
  14. WHO 2000 Overcoming Antimicrobial Resistance, World Health Organization Report on Infectious Diseases 2000 Geneva: World Health Organization; http://www.who.int/infectious-disease-report/2000/other_versions/index-rpt2000_text.html
    [Google Scholar]
  15. Witte W. 2000; Diagnostics, typing and taxonomy. In Gram-Positive Pathogens pp 309–317 Edited by Fischetti V. A., Novick R. P., Ferretti J. J., Portnoy D. A., Rood J. I. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  16. Witte W., Enright M., Schmitz F. J., Cuny C., Braulke C., Heuck D. 2001; Characteristics of a new epidemic MRSA in Germany ancestral to United Kingdom EMRSA-15. Int J Med Microbiol 290:677–682 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.2008/003509-0
Loading
/content/journal/jmm/10.1099/jmm.0.2008/003509-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error