1887

Abstract

Polyhexamethylene guanidine hydrochloride (PHMGH), an antimicrobial biocide of the guanidine family, was tested for efficacy against quality-control strains of , , , meticillin-resistant (MRSA) and . Bactericidal activity against , and was determined using the official methods of analysis of the Association of Official Analytical Chemists, with modifications as recommended by the Canadian General Standards Board. For MRSA and , the MIC and minimal bactericidal concentration were determined using the broth dilution technique. The experiments were carried out at 20 °C under a range of conditions including varying PHMGH concentration (0.001–0.1 %), contact time (0.5–10 min) and water type (distilled, tap and hard water). The phenol coefficient values determined with and were 7.5, 6.1 and 5, respectively. No matter what type of water was used to make the dilutions, PHMGH killed MRSA and at concentrations as low as 0.04 and 0.005 % (w/v), respectively, within 1.5 min. The mode of action of PHMGH was elucidated by transmission electron microscopy: the cell envelope was broken, resulting in cell content leakage into the medium. The ultimate aim of this study was to show that PHMGH can be used as an odourless, colourless, non-corrosive and harmless disinfectant for hospital and household facilities.

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2008-12-01
2019-10-18
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