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Volume 57, Issue 11

Molecular analysis of the glyceraldehyde-3-phosphate dehydrogenase-encoding gene () and differential expression of and the isocitrate lyase-encoding gene () upon internalization by murine macrophages Free

Abstract

is an intracellular dimorphic fungus that can cause a fatal disseminated disease in human immunodeficiency virus-infected patients. The factors that affect the pathogenicity of this fungus remain unclear. Here, we report the isolation and characterization of the cDNA and genomic clones encoding glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in . Phylogenetic analysis of GAPDH amino acid sequences demonstrated the evolutionary relationship of to other fungi, including the intracellular pathogen . To assess the central importance of phagocytic cells in defence against infection, we used Northern blotting to investigate the response of the isocitrate lyase-encoding gene () and to nutrient deprivation inside macrophages. The results revealed that after macrophage internalization, the gene involved in the glyoxylate cycle, , showed higher expression levels as early as 2 h from the start of co-incubation, and the differential expression could be observed again at 8 h after infection. In contrast, the expression of was downregulated in the yeast phase, as well as during macrophage infection after 2, 4 and 8 h of infection. The induction of was shown to be coordinated with the downregulation of the glycolytic gene, implying that the cytoplasmic environment of macrophages is deficient in glucose and the glyoxylate pathway could be used by this pathogen to allow subsistence on two-carbon compounds within the host cell following its intracellular persistence.

  • Received:
  • Accepted:
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Keyword(s): GAPDH, glyceraldehyde-3-phosphate dehydrogenase
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2008-11-01
2024-04-19
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Molecular analysis of the Penicillium marneffei glyceraldehyde-3-phosphate dehydrogenase-encoding gene (gpdA) and differential expression of gpdA and the isocitrate lyase-encoding gene (acuD) upon internalization by murine macrophages
J. Med. Microbiol. 57, 1322 (2008); https://doi.org/10.1099/jmm.0.2008/002832-0
/content/journal/jmm/10.1099/jmm.0.2008/002832-0
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