1887

Abstract

A total of 118 meticillin-resistant (MRSA) and 140 meticillin-susceptible (MSSA) isolates from different patients in the same time period were comprehensively searched using a multiplex PCR for the classical and recently described superantigenic toxin gene family comprising the staphylococcal enterotoxin genes to and the toxic shock syndrome toxin 1 gene, . Both MRSA and MSSA isolates carried a number of superantigenic toxin genes, but the MRSA isolates harboured more superantigenic toxin genes than the MSSA isolates. The most frequent genotype of the MRSA isolates was , and together with the gene combination , , , and , which was found strictly in combination in 69.5 % of the isolates tested. In contrast, possession of the , and genes in MSSA isolates was significantly less than in MRSA (2.1 vs 77.1 %, respectively), although they also often contained the combination genes (25.0 %). This notable higher prevalence in MRSA isolates indicated that possession of the , and genes in particular appeared to be a habitual feature of MRSA. Moreover, these were mainly due to the fixed combinations of the mobile genetic elements type I νSa4 encoding , and , and type I νSa encoding , , , and . Analysis of the relationship between toxin genotypes and the toxin gene-encoding profiles of mobile genetic elements has a possible role in determining superantigenic toxin genotypes in .

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2008-09-01
2020-09-27
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