1887

Abstract

Currently, serovar Brandenburg is identified serologically on the basis of two surface antigens, somatic (O) polysaccharide and flagellar (H) proteins. This procedure is time-consuming and requires expensive typing reagents. To overcome these problems, a PCR method was developed and validated for the identification of Brandenburg. Portions of the , (B), and genes were targeted for amplification using four pairs of oligonucleotide primers. To validate the assay, genomic DNA from an array of 72 strains representing 28 serotypes and 5 non- strains from 4 different genera was subjected to PCR. The four targeted genes were correctly amplified only from Brandenburg. These results indicate that this PCR assay is a simple, rapid, reliable and reproducible method for the identification of Brandenburg that will aid in surveillance, prevention and control of this pathogen.

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2008-10-01
2020-08-14
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