RT Journal Article SR Electronic(1) A1 Varela, Gustavo A1 González, Sabina A1 Gadea, Pilar A1 Coitinho, Cecilia A1 Mota, Inés A1 González, Gladys A1 Goñi, Fernando A1 Rivas, Carlos A1 Schelotto, FelipeYR 2008 T1 Prevalence and dissemination of the Ser315Thr substitution within the KatG enzyme in isoniazid-resistant strains of Mycobacterium tuberculosis isolated in Uruguay JF Journal of Medical Microbiology, VO 57 IS 12 SP 1518 OP 1522 DO https://doi.org/10.1099/jmm.0.2008/001917-0 PB Microbiology Society, SN 1473-5644, AB The aim of this study was to determine the prevalence of Ser315Thr substitution in isoniazid (INH)-resistant strains of Mycobacterium tuberculosis in Uruguay. The katG gene of 62 INH-resistant strains was analysed by an RFLP-PCR assay. PCR products were digested with MspI to detect Ser315Thr and Arg463Leu substitutions. A total of 16 of the 62 (26 %) INH-resistant strains analysed had a Ser315Thr substitution. Only one INH-resistant strain had an Arg463Leu substitution and two strains had a deletion in katG. Of the 16 strains with Ser315Thr, 15 showed different profiles using a double-repetitive-element PCR assay, demonstrating that there was no local dissemination of any particular strain. These findings are in agreement with published data from regions where the prevalence of tuberculosis (TB) is intermediate and may be due in part to the success of the local TB control programme., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.2008/001917-0