1887

Abstract

Penicillin resistance mediated by -lactamase activity has been reported previously in the anaerobic intestinal spirochaete , and a novel class D -lactamase (OXA-63) hydrolysing oxacillin was described recently in a resistant human strain from France. In the current study, 18 strains from Australia and Papua New Guinea were tested for ampicillin and oxacillin susceptibility, and investigated for the presence of the class D -lactamase gene using PCR. PCR products were amplified from seven human and four porcine strains that were penicillin resistant, but not from seven penicillin-sensitive strains. Sequence analysis of the whole gene amplified from seven of the resistant strains from humans and pigs revealed only minor nucleotide differences among them, but there were significant differences compared with . The predicted amino acid sequence of the enzyme from all seven strains had the same key structural motifs as the previously reported OXA-63, but two variants with 94–95 % identity with OXA-63 were identified. OXA-136 had an additional amino acid and 12 other consistent amino acid substitutions compared with OXA-63. OXA-137 had the same differences compared with OXA-63 as OXA-136, but had an additional amino acid substitution at position 16. No structures consistent with integrons or transposons were found in the nucleotide sequences in the vicinity of in partially sequenced strain 95/1000, and the GC content (25.2 mol%) of the gene was similar to that of the whole genome. The gene encoding OXA-136 from strain Cof-10 conferred penicillin resistance on . This study shows that penicillin resistance in human and porcine strains from Australia is associated with the production of two variants of OXA-63, and that susceptible strains lack the genes encoding OXA-63 or the variants.

Keyword(s): PNG, Papua New Guinea
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2008-09-01
2019-10-24
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References

  1. Ambler, R. P. ( 1980; ). The structure of beta-lactamases. Philos Trans R Soc Lond B Biol Sci 289, 321–331.[CrossRef]
    [Google Scholar]
  2. Brooke, C. J., Clair, A. N., Mikosza, A. S. J., Riley, T. V. & Hampson, D. J. ( 2001; ). Carriage of intestinal spirochaetes by humans: epidemiological data from Western Australia. Epidemiol Infect 127, 369–374.
    [Google Scholar]
  3. Brooke, C. J., Hampson, D. J. & Riley, T. V. ( 2003; ). In vitro antimicrobial susceptibility of Brachyspira pilosicoli isolates from humans. Antimicrob Agents Chemother 47, 2354–2357.[CrossRef]
    [Google Scholar]
  4. Brooke, C. J., Riley, T. V. & Hampson, D. J. ( 2006; ). Comparison of prevalence and risk factors for faecal carriage of the intestinal spirochaetes Brachyspira aalborgi and Brachyspira pilosicoli in four Australian populations. Epidemiol Infect 134, 627–634.[CrossRef]
    [Google Scholar]
  5. Bush, K., Jacoby, G. A. & Madeiros, A. A. ( 1995; ). A functional classification scheme for β-lactamases and its correlation with molecular structure. Antimicrob Agents Chemother 39, 1211–1233.[CrossRef]
    [Google Scholar]
  6. CLSI ( 2007; ). Methods for Antimicrobial Susceptibility Testing of Anaerobic Bacteria. Document M11-A7. Wayne, PA: Clinical and Laboratory Standards Institute.
  7. Dassanayake, R. P., Sarath, G. & Duhamel, G. E. ( 2005; ). Penicillin-binding proteins in the pathogenic intestinal spirochete Brachyspira pilosicoli. Antimicrob Agents Chemother 49, 1561–1563.[CrossRef]
    [Google Scholar]
  8. Douglas, J. G. & Crucioli, V. ( 1981; ). Spirochaetosis: a remedial cause of diarrhoea and rectal bleeding? Br Med J (Clin Res Ed) 283, 1362 [CrossRef]
    [Google Scholar]
  9. Esteve, M., Salas, A., Fernandez-Banares, F., Lloreta, J., Marine, M., Gonzalez, C. I., Forne, M., Casalots, J., Santaolalla, R. & other authors ( 2006; ). Intestinal spirochetosis and chronic watery diarrhea: clinical and histological response to treatment and long-term follow up. J Gastroenterol Hepatol 21, 1326–1333.[CrossRef]
    [Google Scholar]
  10. Fournié-Amazouz, E., Baranton, G., Carlier, J. P., Chambreuil, G., Cohadon, F., Collin, P., Jolivet, A. G., Hermes, I., Lemarie, C. & Saint Girons, I. ( 1995; ). Isolations of intestinal spirochaetes from the blood of human patients. J Hosp Infect 30, 160–162.[CrossRef]
    [Google Scholar]
  11. Hampson, D. J. & Duhamel, G. E. ( 2006; ). Porcine colonic spirochetosis/intestinal spirochetosis. In Diseases of Swine, 9th edn, pp. 755–767. Edited by B. E. Straw, J. J. Zimmerman, S. D'Allaire & D. J. Taylor. Oxford: Blackwell Publishing.
  12. Hampson, D. J., Oxberry, S. L. & La, T. ( 2006a; ). Potential for zoonotic transmission of Brachyspira pilosicoli. Emerg Infect Dis 12, 869–870.[CrossRef]
    [Google Scholar]
  13. Hampson, D. J., Stephens, C. P. & Oxberry, S. L. ( 2006b; ). Antimicrobial susceptibility testing of Brachyspira intermedia and Brachyspira pilosicoli isolates from Australian chickens. Avian Pathol 35, 12–16.[CrossRef]
    [Google Scholar]
  14. Harland, W. A. & Lee, F. D. ( 1967; ). Intestinal spirochaetosis. BMJ 3, 718–719.[CrossRef]
    [Google Scholar]
  15. Kanavaki, S., Mantadakis, E., Thomakos, N., Pefanis, A., Matsiota-Bernard, P., Karabela, S. & Samonis, G. ( 2002; ). Brachyspira (Serpulina) pilosicoli spirochetemia in an immunocompromised patient. Infection 30, 175–177.[CrossRef]
    [Google Scholar]
  16. Law, C. L. H., Grierson, J. M. & Stevens, S. M. B. ( 1994; ). Rectal spirochaetosis in homosexual men: the association with sexual practices, HIV infection and enteric flora. Genitourin Med 70, 26–29.
    [Google Scholar]
  17. Lee, J. I. & Hampson, D. J. ( 1992; ). Intestinal spirochaetes colonizing Aborigines from communities in the remote north of Western Australia. Epidemiol Infect 109, 133–141.
    [Google Scholar]
  18. Lee, J. I. & Hampson, D. J. ( 1994; ). Genetic characterisation of intestinal spirochaetes and their association with disease. J Med Microbiol 40, 365–371.[CrossRef]
    [Google Scholar]
  19. Lima, M. A., Barbosa, A. L., Santos, V. M. & Misiara, F. P. ( 2005; ). Intestinal spirochetosis and colon diverticulosis. Rev Soc Bras Med Trop 38, 56–57.
    [Google Scholar]
  20. Margawani, K. R., Robertson, I. D., Brooke, C. J. & Hampson, D. J. ( 2004; ). Prevalence, risk factors and molecular epidemiology of Brachyspira pilosicoli in humans on the island of Bali, Indonesia. J Med Microbiol 53, 325–332.[CrossRef]
    [Google Scholar]
  21. Matson, E. G., Thompson, M. G., Humphrey, S. B., Zuerner, R. L. & Stanton, T. B. ( 2005; ). Identification of genes of VSH-1, a prophage-like gene transfer agent of Brachyspira hyodysenteriae. J Bacteriol 187, 5885–5892.[CrossRef]
    [Google Scholar]
  22. Meziane-Cherif, D., Lambert, T., Dupechez, M., Courvalin, P. & Galimand, M. ( 2008; ). Genetic and biochemical characterization of OXA-63, a new class D β-lactamase from Brachyspira pilosicoli BM4442. Antimicrob Agents Chemother 52, 1264–1268.[CrossRef]
    [Google Scholar]
  23. Mikosza, A. S. J. & Hampson, D. J. ( 2001; ). Human intestinal spirochetosis: Brachyspira aalborgi and/or Brachyspira pilosicoli? Anim Health Res Rev 2, 101–110.
    [Google Scholar]
  24. Munshi, M. A., Traub, R. J., Robertson, I. D., Mikosza, A. S. J. & Hampson, D. J. ( 2004; ). Colonization and risk factors for Brachyspira aalborgi and Brachyspira pilosicoli in humans and dogs on tea-estates in Assam, India. Epidemiol Infect 132, 137–144.[CrossRef]
    [Google Scholar]
  25. Oxberry, S. L., Trott, D. J. & Hampson, D. J. ( 1998; ). Serpulina pilosicoli, water birds and water: potential sources of infection for humans and other animals. Epidemiol Infect 121, 219–225.[CrossRef]
    [Google Scholar]
  26. Rozen, S. & Skaletsky, H. J. ( 2000; ). Primer3 on the WWW for general users and for biologist programmers. In Bioinformatics Methods and Protocols: Methods in Molecular Biology, pp. 365–386. Edited by S. Krawetz & S. Misoner. Totowa, NJ: Humana Press.
  27. Sambrook, J., Fritsch, E. F. & Maniatis, T. ( 1989; ). Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, USA: Cold Spring Harbor Laboratory.
  28. Stanton, T. B., Thompson, M. G., Humphrey, S. B. & Zuerner, R. L. ( 2003; ). Detection of bacteriophage VSH-1 svp38 gene in Brachyspira spirochetes. FEMS Microbiol Lett 224, 225–229.[CrossRef]
    [Google Scholar]
  29. Sun, T., Nukaga, M., Mayama, K., Braswell, E. H. & Know, J. R. ( 2003; ). Comparison of β-lactamases of classes A and D: 1.5 Å crystallographic structure of the class D OXA-1 oxacillinase. Protein Sci 12, 82–91.[CrossRef]
    [Google Scholar]
  30. Tompkins, D. S., Millar, M., Heritage, J. & West, A. P. ( 1987; ). β-Lactamase production by intestinal spirochaetes. J Gen Microbiol 133, 761–765.
    [Google Scholar]
  31. Trivett-Moore, N. L., Gilbert, G. L., Law, C. L. H., Trott, D. J. & Hampson, D. J. ( 1998; ). Isolation of Serpulina pilosicoli from rectal biopsy specimens showing evidence of intestinal spirochetosis. J Clin Microbiol 36, 261–265.
    [Google Scholar]
  32. Trott, D. J., Combs, B. G., Oxberry, S. L., Mikosza, A. S. J., Robertson, I. D., Passey, M., Taime, J., Sehuko, R. & Hampson, D. J. ( 1997a; ). The prevalence of Serpulina pilosicoli in humans and domestic animals in the Eastern Highlands of Papua New Guinea. Epidemiol Infect 119, 369–379.[CrossRef]
    [Google Scholar]
  33. Trott, D. J., Jensen, N. S., Saint Girons, I., Oxberry, S. L., Stanton, T. B., Lindquist, D. & Hampson, D. J. ( 1997b; ). Identification and characterization of Serpulina pilosicoli isolates from the blood of critically-ill patients. J Clin Microbiol 35, 482–485.
    [Google Scholar]
  34. Trott, D. J., Mikosza, A. S. J., Combs, B. G., Oxberry, S. L. & Hampson, D. J. ( 1998; ). Population genetic analysis of Serpulina pilosicoli and its molecular epidemiology in villages in the Eastern Highlands of Papua New Guinea. Int J Syst Bacteriol 48, 659–668.[CrossRef]
    [Google Scholar]
  35. Voha, C., Docquier, J.-D., Rossolini, G. M. & Fosse, T. ( 2006; ). Genetic and biochemical characterization of FUS-1 (OXA-85), a narrow-spectrum class D β-lactamase from Fusobacterium nucleatum subsp. polymorphum. Antimicrob Agents Chemother 50, 2673–2679.[CrossRef]
    [Google Scholar]
  36. Williams, P., Gracey, M. & Smith, P. ( 1997; ). Hospitalization of Aboriginal and non-Aboriginal patients for respiratory tract diseases in Western Australia, 1988–1993. Int J Epidemiol 26, 797–805.[CrossRef]
    [Google Scholar]
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