1887

Abstract

We recently showed that OmcB protein from serovar LGV1 functions as an adhesin. In this study, we produced expressing OmcB from serovar E and compared this OmcB to OmcB from serovar LGV1. Infectivity inhibition assays carried out with serovars LGV1 and E of in the presence of recombinant OmcB showed considerable (∼60 %) inhibition of infectivity. In the presence of heparan sulphate, there was significant inhibition (68 %) of adherence of expressing OmcB from serovar LGV1 only. In a further experiment, recombinant OmcB from serovar LGV1 showed minimal binding to glycosaminoglycan (GAG)-deficient cells, whilst to the same cells, recombinant OmcB from serovar E showed binding equal to that to the wild-type cells. Our experiments strongly suggest that OmcB from serovar E, in contrast to that from serovar LGV1, is not binding to host cells through a GAG-dependent mechanism.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.2008/001305-0
2008-09-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/57/9/1058.html?itemId=/content/journal/jmm/10.1099/jmm.0.2008/001305-0&mimeType=html&fmt=ahah

References

  1. Bradford M. M. 1976; A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254 [CrossRef]
    [Google Scholar]
  2. Chen J. C.-R., Stephens R. S. 1994; Trachoma and LGV biovars of Chlamydia trachomatis share the same glycosaminoglycan dependent mechanism for infection of eukaryotic cells. Mol Microbiol 11:501–507 [CrossRef]
    [Google Scholar]
  3. Chen J. C.-R., Stephens R. S. 1997; Chlamydia trachomatis glycosaminoglycan-dependent and independent attachment to eukaryotic cells. Microb Pathog 22:23–30 [CrossRef]
    [Google Scholar]
  4. Davis C. H., Wyrick P. B. 1997; Differences in the association of Chlamydia trachomatis serovar E and serovar L2 with epithelial cells in vitro may reflect biological differences in vivo . Infect Immun 65:2914–2924
    [Google Scholar]
  5. Fadel S., Eley A. 2004; Chlorate: a reversible inhibitor of proteoglycan sulphation in Chlamydia trachomatis -infected cells. J Med Microbiol 53:93–95 [CrossRef]
    [Google Scholar]
  6. Fadel S., Eley A. 2007; Chlamydia trachomatis OmcB protein is a surface-exposed glycosaminoglycan-dependent adhesin. J Med Microbiol 56:15–22 [CrossRef]
    [Google Scholar]
  7. Moelleken K., Hegemann J. H. 2008; The Chlamydia outer membrane protein OmcB is required for adhesion and exhibits biovar-specific differences in glycosaminoglycan binding. Mol Microbiol 67:403–419
    [Google Scholar]
  8. Moulder J. W. 1991; Interaction of chlamydiae and the host cells in vitro . Microbiol Rev 55:143–190
    [Google Scholar]
  9. Stephens R. S., Fawaz F. S., Kennedy K. A., Koshiyama K., Nichols B., van Ooij C., Engel J. N. 2000; Eukaryotic cell uptake of heparin-coated microspheres: a model of host cell invasion by Chlamydia trachomatis . Infect Immun 68:1080–1085 [CrossRef]
    [Google Scholar]
  10. Stephens R. S., Koshiyama K., Lewis E., Kubo A. 2001; Heparin-binding outer membrane protein of chlamydiae. Mol Microbiol 40:691–699 [CrossRef]
    [Google Scholar]
  11. Stephens R. S., Poteralski J. M., Olinger L. 2006; Interaction of Chlamydia trachomatis with mammalian cells is independent of host cell surface heparin sulfate glycosaminoglycans. Infect Immun 74:1795–1799 [CrossRef]
    [Google Scholar]
  12. Taraktchoglou M., Pacey A. A., Turnbull J. E., Eley A. 2001; Infectivity of Chlamydia trachomatis serovar LGV but not E is dependent on host cell heparan sulfate. Infect Immun 69:968–976 [CrossRef]
    [Google Scholar]
  13. Thornley J. P., Shaw J. G., Gryllos I. A., Eley A. 1996; Adherence of Aeromonas caviae to human cell lines HEp-2 and Caco-2. J Med Microbiol 45:445–451 [CrossRef]
    [Google Scholar]
  14. Zhang J. P., Stephens R. S. 1992; Mechanism of C. trachomatis attachment to eukaryotic host cells. Cell 69:861–869 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.2008/001305-0
Loading
/content/journal/jmm/10.1099/jmm.0.2008/001305-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error