RT Journal Article SR Electronic(1) A1 Zhang, Ying A1 Zhou, Hua A1 Shen, Xiao-qiang A1 Shen, Ping A1 Yu, Yun-song A1 Li, Lan-juanYR 2008 T1 Plasmid-borne armA methylase gene, together with blaCTX-M-15 and blaTEM-1, in a Klebsiella oxytoca isolate from China JF Journal of Medical Microbiology, VO 57 IS 10 SP 1273 OP 1276 DO https://doi.org/10.1099/jmm.0.2008/001271-0 PB Microbiology Society, SN 1473-5644, AB An armA-producing Klebsiella oxytoca isolate, strain 157, was detected after screening of 447 extended-spectrum β-lactamase-producing Enterobacteriaceae isolates in China. K. oxytoca 157 was resistant to aminoglycosides, ciprofloxacin and most β-lactams. Resistance to aminoglycosides and β-lactams could be transferred to recipient Escherichia coli by conjugation. armA, bla CTX-M-15 and bla TEM-1 genes were detected in K. oxytoca 157 and transconjugant E. coli strain 600(pEC157). Mutation of aa 87 in GyrA was found in K. oxytoca 157. A plasmid of ∼55 kb was extracted from K. oxytoca 157(pKO157) and E. coli 600(pEC157). Southern blot hybridization confirmed that the armA, bla CTX-M-15 and bla TEM-1 genes were all located on this conjugative plasmid (pEC157). PCR mapping was also performed to investigate the genetic environment of armA. The armA gene was found to be flanked by the same putative transposable elements as reported previously in E. coli, Klebsiella pneumoniae and Citrobacter freundii isolates from different countries., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.2008/001271-0