1887

Abstract

An -producing isolate, strain 157, was detected after screening of 447 extended-spectrum -lactamase-producing isolates in China. 157 was resistant to aminoglycosides, ciprofloxacin and most -lactams. Resistance to aminoglycosides and -lactams could be transferred to recipient by conjugation. , and genes were detected in 157 and transconjugant strain 600(pEC157). Mutation of aa 87 in GyrA was found in 157. A plasmid of ∼55 kb was extracted from 157(pKO157) and 600(pEC157). Southern blot hybridization confirmed that the , and genes were all located on this conjugative plasmid (pEC157). PCR mapping was also performed to investigate the genetic environment of . The gene was found to be flanked by the same putative transposable elements as reported previously in , and isolates from different countries.

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2008-10-01
2020-08-08
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