1887

Abstract

An -producing isolate, strain 157, was detected after screening of 447 extended-spectrum -lactamase-producing isolates in China. 157 was resistant to aminoglycosides, ciprofloxacin and most -lactams. Resistance to aminoglycosides and -lactams could be transferred to recipient by conjugation. , and genes were detected in 157 and transconjugant strain 600(pEC157). Mutation of aa 87 in GyrA was found in 157. A plasmid of ∼55 kb was extracted from 157(pKO157) and 600(pEC157). Southern blot hybridization confirmed that the , and genes were all located on this conjugative plasmid (pEC157). PCR mapping was also performed to investigate the genetic environment of . The gene was found to be flanked by the same putative transposable elements as reported previously in , and isolates from different countries.

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2008-10-01
2019-11-17
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References

  1. Chen, L., Chen, Z. L., Liu, J. H., Zeng, Z. L., Ma, J. Y. & Jiang, H. X. ( 2007; ). Emergence of RmtB methylase-producing Escherichia coli and Enterobacter cloacae isolates from pigs in China. J Antimicrob Chemother 59, 880–885.[CrossRef]
    [Google Scholar]
  2. CLSI ( 2006; ). Performance Standards for Antimicrobial Susceptibility Testing. Sixteenth Informational Supplement, M100-S16. Wayne, PA: Clinical and Laboratory Standards Institute.
  3. Decré, D., Burghoffer, B., Gautier, V., Petit, J. C. & Arlet, G. ( 2004; ). Outbreak of multi-resistant Klebsiella oxytoca involving strains with extended-spectrum β-lactamases and strains with extended-spectrum activity of the chromosomal β-lactamase. J Antimicrob Chemother 54, 881–888.[CrossRef]
    [Google Scholar]
  4. Doi, Y., Yokoyama, K., Yamane, K., Wachino, J., Shibata, N., Yagi, T., Shibayama, K., Kato, H. & Arakawa, Y. ( 2004; ). Plasmid-mediated 16S rRNA methylase in Serratia marcescens conferring high-level resistance to aminoglycosides. Antimicrob Agents Chemother 48, 491–496.[CrossRef]
    [Google Scholar]
  5. Galimand, M., Courvalin, P. & Lambert, T. ( 2003; ). Plasmid-mediated high-level resistance to aminoglycosides in Enterobacteriaceae due to 16S rRNA methylation. Antimicrob Agents Chemother 47, 2565–2571.[CrossRef]
    [Google Scholar]
  6. Galimand, M., Sabtcheva, S., Courvalin, P. & Lambert, T. ( 2005; ). Worldwide disseminated armA aminoglycoside resistance methylase gene is borne by composite transposon Tn1548. Antimicrob Agents Chemother 49, 2949–2953.[CrossRef]
    [Google Scholar]
  7. Gomez-Flores, A., Welsh, O., Said-Fernández, S., Lozano-Garza, G., Tavarez-Alejandro, R. E. & Vera-Cabrera, L. ( 2004; ). In vitro and in vivo activities of antimicrobials against Nocardia brasiliensis. Antimicrob Agents Chemother 48, 832–837.[CrossRef]
    [Google Scholar]
  8. González-Zorn, B., Catalan, A., Escudero, J. A., Dominguez, L., Teshager, T., Porrero, C. & Moreno, M. A. ( 2005; ). Genetic basis for dissemination of armA. J Antimicrob Chemother 56, 583–585.[CrossRef]
    [Google Scholar]
  9. Livermore, D. M. ( 1995; ). β-Lactamases in laboratory and clinical resistance. Clin Microbiol Rev 8, 557–584.
    [Google Scholar]
  10. Maurin, M., Abergel, C. & Raoult, D. ( 2001; ). DNA gyrase-mediated natural resistance to fluoroquinolones in Ehrlichia spp. Antimicrob Agents Chemother 45, 2098–2105.[CrossRef]
    [Google Scholar]
  11. Wachino, J., Shibayama, K., Kurokawa, H., Kimura, K., Yamane, K., Suzuki, S., Shibata, N., Ike, Y. & Arakawa, Y. ( 2007; ). Novel plasmid-mediated 16S rRNA m1A1408 methyltransferase, NpmA, found in a clinically isolated Escherichia coli strain resistant to structurally diverse aminoglycosides. Antimicrob Agents Chemother 51, 4401–4409.[CrossRef]
    [Google Scholar]
  12. Yamane, K., Wachino, J., Doi, Y., Kurokawa, H. & Arakawa, Y. ( 2005; ). Global spread of multiple aminoglycoside resistance genes. Emerg Infect Dis 11, 951–953.[CrossRef]
    [Google Scholar]
  13. Yan, J. J., Ko, W. C. & Wu, J. J. ( 2001; ). Identification of a plasmid encoding SHV-12, TEM-1, and a variant of IMP-2 metallo-β-lactamase, IMP-8, from a clinical isolate of Klebsiella pneumoniae. Antimicrob Agents Chemother 45, 2368–2371.[CrossRef]
    [Google Scholar]
  14. Yan, J. J., Wu, J. J., Ko, W. C., Tsai, S. H., Chuang, C. L., Wu, H. M., Lu, Y. J. & Li, J. D. ( 2004; ). Plasmid-mediated 16S rRNA methylases conferring high-level aminoglycoside resistance in Escherichia coli and Klebsiella pneumoniae isolates from two Taiwanese hospitals. J Antimicrob Chemother 54, 1007–1012.[CrossRef]
    [Google Scholar]
  15. Yokoyama, K., Doi, Y., Yamane, K., Kurokawa, H., Shibata, N., Shibayama, K., Yagi, T., Kato, H. & Arakawa, Y. ( 2003; ). Acquisition of 16S rRNA methylase gene in Pseudomonas aeruginosa. Lancet 362, 1888–1893.[CrossRef]
    [Google Scholar]
  16. Yu, Y., Ji, S., Chen, Y., Zhou, W., Wei, Z., Li, L. & Ma, Y. ( 2007a; ). Resistance of strains producing extended-spectrum β-lactamases and genotype distribution in China. J Infect 54, 53–57.[CrossRef]
    [Google Scholar]
  17. Yu, Y. S., Zhou, H., Yang, Q., Chen, Y. G. & Li, L. J. ( 2007b; ). Widespread occurrence of aminoglycoside resistance due to ArmA methylase in imipenem-resistant Acinetobacter baumannii isolates in China. J Antimicrob Chemother 60, 454–455.[CrossRef]
    [Google Scholar]
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