1887

Abstract

An -producing isolate, strain 157, was detected after screening of 447 extended-spectrum -lactamase-producing isolates in China. 157 was resistant to aminoglycosides, ciprofloxacin and most -lactams. Resistance to aminoglycosides and -lactams could be transferred to recipient by conjugation. , and genes were detected in 157 and transconjugant strain 600(pEC157). Mutation of aa 87 in GyrA was found in 157. A plasmid of ∼55 kb was extracted from 157(pKO157) and 600(pEC157). Southern blot hybridization confirmed that the , and genes were all located on this conjugative plasmid (pEC157). PCR mapping was also performed to investigate the genetic environment of . The gene was found to be flanked by the same putative transposable elements as reported previously in , and isolates from different countries.

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2008-10-01
2024-04-25
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References

  1. Chen L., Chen Z. L., Liu J. H., Zeng Z. L., Ma J. Y., Jiang H. X. 2007; Emergence of RmtB methylase-producing Escherichia coli and Enterobacter cloacae isolates from pigs in China. J Antimicrob Chemother 59:880–885 [CrossRef]
    [Google Scholar]
  2. CLSI 2006 Performance Standards for Antimicrobial Susceptibility Testing . Sixteenth Informational Supplement, M100-S16 Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  3. Decré D., Burghoffer B., Gautier V., Petit J. C., Arlet G. 2004; Outbreak of multi-resistant Klebsiella oxytoca involving strains with extended-spectrum β -lactamases and strains with extended-spectrum activity of the chromosomal β -lactamase. J Antimicrob Chemother 54:881–888 [CrossRef]
    [Google Scholar]
  4. Doi Y., Yokoyama K., Yamane K., Wachino J., Shibata N., Yagi T., Shibayama K., Kato H., Arakawa Y. 2004; Plasmid-mediated 16S rRNA methylase in Serratia marcescens conferring high-level resistance to aminoglycosides. Antimicrob Agents Chemother 48:491–496 [CrossRef]
    [Google Scholar]
  5. Galimand M., Courvalin P., Lambert T. 2003; Plasmid-mediated high-level resistance to aminoglycosides in Enterobacteriaceae due to 16S rRNA methylation. Antimicrob Agents Chemother 47:2565–2571 [CrossRef]
    [Google Scholar]
  6. Galimand M., Sabtcheva S., Courvalin P., Lambert T. 2005; Worldwide disseminated armA aminoglycoside resistance methylase gene is borne by composite transposon Tn 1548 . Antimicrob Agents Chemother 49:2949–2953 [CrossRef]
    [Google Scholar]
  7. Gomez-Flores A., Welsh O., Said-Fernández S., Lozano-Garza G., Tavarez-Alejandro R. E., Vera-Cabrera L. 2004; In vitro and in vivo activities of antimicrobials against Nocardia brasiliensis . Antimicrob Agents Chemother 48:832–837 [CrossRef]
    [Google Scholar]
  8. González-Zorn B., Catalan A., Escudero J. A., Dominguez L., Teshager T., Porrero C., Moreno M. A. 2005; Genetic basis for dissemination of armA . J Antimicrob Chemother 56:583–585 [CrossRef]
    [Google Scholar]
  9. Livermore D. M. 1995; β -Lactamases in laboratory and clinical resistance. Clin Microbiol Rev 8:557–584
    [Google Scholar]
  10. Maurin M., Abergel C., Raoult D. 2001; DNA gyrase-mediated natural resistance to fluoroquinolones in Ehrlichia spp. Antimicrob Agents Chemother 45:2098–2105 [CrossRef]
    [Google Scholar]
  11. Wachino J., Shibayama K., Kurokawa H., Kimura K., Yamane K., Suzuki S., Shibata N., Ike Y., Arakawa Y. 2007; Novel plasmid-mediated 16S rRNA m1A1408 methyltransferase, NpmA, found in a clinically isolated Escherichia coli strain resistant to structurally diverse aminoglycosides. Antimicrob Agents Chemother 51:4401–4409 [CrossRef]
    [Google Scholar]
  12. Yamane K., Wachino J., Doi Y., Kurokawa H., Arakawa Y. 2005; Global spread of multiple aminoglycoside resistance genes. Emerg Infect Dis 11:951–953 [CrossRef]
    [Google Scholar]
  13. Yan J. J., Ko W. C., Wu J. J. 2001; Identification of a plasmid encoding SHV-12, TEM-1, and a variant of IMP-2 metallo- β -lactamase, IMP-8, from a clinical isolate of Klebsiella pneumoniae . Antimicrob Agents Chemother 45:2368–2371 [CrossRef]
    [Google Scholar]
  14. Yan J. J., Wu J. J., Ko W. C., Tsai S. H., Chuang C. L., Wu H. M., Lu Y. J., Li J. D. 2004; Plasmid-mediated 16S rRNA methylases conferring high-level aminoglycoside resistance in Escherichia coli and Klebsiella pneumoniae isolates from two Taiwanese hospitals. J Antimicrob Chemother 54:1007–1012 [CrossRef]
    [Google Scholar]
  15. Yokoyama K., Doi Y., Yamane K., Kurokawa H., Shibata N., Shibayama K., Yagi T., Kato H., Arakawa Y. 2003; Acquisition of 16S rRNA methylase gene in Pseudomonas aeruginosa . Lancet 362:1888–1893 [CrossRef]
    [Google Scholar]
  16. Yu Y., Ji S., Chen Y., Zhou W., Wei Z., Li L., Ma Y. 2007a; Resistance of strains producing extended-spectrum β -lactamases and genotype distribution in China. J Infect 54:53–57 [CrossRef]
    [Google Scholar]
  17. Yu Y. S., Zhou H., Yang Q., Chen Y. G., Li L. J. 2007b; Widespread occurrence of aminoglycoside resistance due to ArmA methylase in imipenem-resistant Acinetobacter baumannii isolates in China. J Antimicrob Chemother 60:454–455 [CrossRef]
    [Google Scholar]
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