1887

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Volume 57, Issue 10

Phenotypic evaluation of the effect of anaerobiosis on some virulence attributes of Free

Abstract

The current assumption that is a facultatively anaerobic organism has been widely accepted since its recovery from anoxic sites became common. However, the link between anaerobiosis and virulence remains uncertain. This study investigated the differential cell-surface hydrophobicity (CSH) using a hydrocarbon/water partition technique and analysed the differential secretion rates of secretory aspartyl proteases (Saps), esterase, chondroitinase and haemolysins of strains recovered from periodontal pockets and non-periodontium-related intra-oral sites. For the enzymic tests, all strains from both sets were grown under aerobic and anaerobic conditions and the harvested cells were inoculated onto suitable normal or pre-reduced culture media in the presence or absence of molecular oxygen, respectively. The results showed that no variations were perceptible for CSH and chondroitinase ( >0.05). The secretion rates of esterase and haemolysins strongly decreased in an anoxic environment ( <0.0001). However, a consistent increment ( <0.0001) in Sap secretion was detected when cultures were grown under anaerobic conditions. Based on these results, it is suggested that the oxygen concentration in the atmosphere surrounding cells exerts a variable influence on the virulence attributes of .

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Keyword(s): CSH, cell-surface hydrophobicity and Sap, secretory aspartyl protease
SGM
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2008-10-01
2024-04-19
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Phenotypic evaluation of the effect of anaerobiosis on some virulence attributes of Candida albicans
J. Med. Microbiol. 57, 1277 (2008); https://doi.org/10.1099/jmm.0.2008/001107-0
/content/journal/jmm/10.1099/jmm.0.2008/001107-0
/content/journal/jmm/10.1099/jmm.0.2008/001107-0
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