1887

Abstract

We have evaluated two simple, rapid and low-cost colorimetric methods for the detection of multidrug-resistant . A total of 151 strains were tested for resistance to rifampicin (RMP) and isoniazid by resazurin microplate assay (REMA) and nitrate reductase assay (NRA) in comparison with the conventional proportion method (PM) on Löwenstein-Jensen medium. A complete agreement was found between NRA and PM, while one false RMP-susceptible result was found by REMA. REMA and NRA tests are rapid and inexpensive, and could be good alternatives to the conventional PM in low-resource countries.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.2008/000406-0
2008-08-01
2024-03-19
Loading full text...

Full text loading...

/deliver/fulltext/jmm/57/8/1024.html?itemId=/content/journal/jmm/10.1099/jmm.0.2008/000406-0&mimeType=html&fmt=ahah

References

  1. Abate G., Aseffa A., Selassie A., Goshu S., Fekade B., WoldeMeskal D., Miörner H. 2004; Direct colorimetric assay for rapid detection of rifampin-resistant Mycobacterium tuberculosis . J Clin Microbiol 42:871–873 [CrossRef]
    [Google Scholar]
  2. Affolabi D., Adjagba O. A., Tanimomo-Kledjo B., Gninafon M., Anagonou S. Y., Portaels F. 2007a; Anti-tuberculosis drug resistance among new and previously treated pulmonary tuberculosis patients in Cotonou, Benin. Int J Tuberc Lung Dis 11:1221–1224
    [Google Scholar]
  3. Affolabi D., Odoun M., Martin A., Palomino J. C., Anagonou S., Portaels F. 2007b; Evaluation of direct detection of Mycobacterium tuberculosis rifampin resistance by a nitrate reductase assay applied to sputum samples in Cotonou, Benin. J Clin Microbiol 45:2123–2125 [CrossRef]
    [Google Scholar]
  4. Altman D. G. 1999 Practical Statistics for Medical Research London: Chapman & Hall/CRC Press;
    [Google Scholar]
  5. Angeby K. A., Klintz L., Hoffner S. E. 2002; Rapid and inexpensive drug susceptibility testing of Mycobacterium tuberculosis with a nitrate reductase assay. J Clin Microbiol 40:553–555 [CrossRef]
    [Google Scholar]
  6. Aziz M. A., Wright A., Laszlo A., De Muynck A., Portaels F., Van Deun A., Wells C., Nunn P., Blanc L. other authors 2006; Epidemiology of antituberculosis drug resistance (the Global Project on Anti-tuberculosis Drug Resistance Surveillance): an updated analysis. Lancet 368:2142–2154 [CrossRef]
    [Google Scholar]
  7. Canetti G., Froman S., Grosset J., Hauduroy P., Langerova M., Mahler H. T., Meissner G., Mitchison D. A., Sula L. 1963; Mycobacteria: laboratory methods for testing drug sensitivity and resistance. Bull World Health Organ 29:565–578
    [Google Scholar]
  8. Canetti G., Fox W., Khomenko A., Mahler H. T., Menon N. K., Mitchison D. A., Rist N., Smelev N. A. 1969; Advances in techniques of testing mycobacterial drug sensitivity, and the use of sensitivity tests in tuberculosis control programmes. Bull World Health Organ 41:21–43
    [Google Scholar]
  9. Caviedes L., Lee T.-S., Gilman R. H., Sheen P., Spellman E., Lee E. H., Berg D. E., Montenegro-James S. the Tuberculosis Working Group in Peru 2000; Rapid, efficient detection and drug susceptibility testing of Mycobacterium tuberculosis in sputum by microscopic observation of broth cultures. J Clin Microbiol 38:1203–1208
    [Google Scholar]
  10. Coban A. Y., Cekic Cihan C., Bilgin K., Uzun M., Akgunes A., Cetinkaya E., Durupinar B. 2006; Rapid susceptibility test for Mycobacterium tuberculosis to isoniazid and rifampin with resazurin method in screw-cap tubes. J Chemother 18:140–143 [CrossRef]
    [Google Scholar]
  11. Kent P. T., Kubica G. P. 1985 Public Health Mycobacteriology. A Guide for the Level III Laboratory Atlanta, GA: Centers for Disease Control and Prevention;
    [Google Scholar]
  12. Musa H. R., Ambroggi M., Souto A., Angeby K. A. 2005; Drug susceptibility testing of Mycobacterium tuberculosis by a nitrate reductase assay applied directly on microscopy-positive sputum samples. J Clin Microbiol 43:3159–3161 [CrossRef]
    [Google Scholar]
  13. Nateche F., Martin A., Baraka S., Palomino J. C., Khaled S., Portaels F. 2006; Application of the resazurin microtitre assay for detection of multidrug resistance in Mycobacterium tuberculosis in Algiers. J Med Microbiol 55:857–860 [CrossRef]
    [Google Scholar]
  14. Palomino J. C., Traore H., Fissette K., Portaels F. 1999; Evaluation of mycobacteria growth indicator tube (MGIT) for drug susceptibility testing of Mycobacterium tuberculosis . Int J Tuberc Lung Dis 3:344–348
    [Google Scholar]
  15. Palomino J. C., Martin A., Camacho M., Guerra H., Swings J., Portaels F. 2002; Resazurin microtiter assay plate: simple and inexpensive method for detection of drug resistance in Mycobacterium tuberculosis . Antimicrob Agents Chemother 46:2720–2722 [CrossRef]
    [Google Scholar]
  16. Rivoire N., Ravololonandriana P., Rasolonavalona T., Martin A., Portaels F., Ramarokoto H., Rasolofo Razanamparany V. 2007; Evaluation of the resazurin assay for the detection of multidrug-resistant Mycobacterium tuberculosis in Madagascar. Int J Tuberc Lung Dis 11:683–688
    [Google Scholar]
  17. Roberts G. D., Goodman N. L., Heifets L., Larsh H. W., Lindner T. H., McClatchy J. K., McGinnis M. R., Siddiqi S. H., Wright P. 1983; Evaluation of the BACTEC radiometric method for recovery of mycobacteria and drug susceptibility testing of Mycobacterium tuberculosis from acid-fast smear-positive specimens. J Clin Microbiol 18:689–696
    [Google Scholar]
  18. Rossau R., Traore H., De Beenhouwer H., Mijs W., Jannes G., De Rijk P., Portaels F. 1997; Evaluation of the INNO-LiPA Rif. TB assay, a reverse hybridization assay for the simultaneous detection of Mycobacterium tuberculosis complex and its resistance to rifampin. Antimicrob Agents Chemother 41:2093–2098
    [Google Scholar]
  19. Shah N. S., Wright A., Bai G. H., Barrera L., Boulahbal F., Martín-Casabona N., Drobniewski F., Gilpin C., Havelková M. other authors 2007; Worldwide emergence of extensively drug-resistant tuberculosis. Emerg Infect Dis 13:380–387 [CrossRef]
    [Google Scholar]
  20. Solis L. A., Shin S. S., Han L. L., Llanos F., Stowell M., Sloutsky A. 2005; Validation of a rapid method for detection of Mycobacterium tuberculosis resistance to isoniazid and rifampin in Lima, Peru. Int J Tuberc Lung Dis 9:760–764
    [Google Scholar]
  21. WHO 2004 The WHO/IUATLD Global Project on Antituberculosis Drug Resistance Surveillance. Antituberculosis Drug Resistance in the World , report no. 3, WHO/HTM/TB/2005.349 Geneva: World Health Organization;
    [Google Scholar]
  22. WHO 2007 Global Tuberculosis Control: Surveillance, Planning, Financing , WHO report 2007, WHO/HTM/TB/2007.376 Geneva: World Health Organization;
    [Google Scholar]
  23. Wilson S. M., Al-Suwaidi Z., McNerney R., Porter J., Drobniewski F. 1997; Evaluation of a new rapid bacteriophage-based method for the drug susceptibility testing of Mycobacterium tuberculosis . Nat Med 3:465–468 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.2008/000406-0
Loading
/content/journal/jmm/10.1099/jmm.0.2008/000406-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error