1887

Abstract

Antimicrobial resistance in is a growing public health concern and an important pathogen in nosocomial infections. We investigated the genes involved in resistance to carbapenems and cephalosporins in clinical isolates from a tertiary medical centre in Malaysia. was isolated from 167 clinical specimens and identified by sequencing of the 16S rRNA and genes. The MIC for imipenem, meropenem, ceftazidime and cefepime were determined by the E-test method. The presence of carbapenemase and cephalosporinase genes was investigated by PCR. The isolates were predominantly nonsusceptible to carbapenems and cephalosporins (>70 %) with high MIC values. IS was detected in all carbapenem-nonsusceptible harbouring the gene. The presence of and IS upstream of was not associated with nonsusceptibility to carbapenems. isolates harbouring IS- (85.8 %) were significantly associated with nonsusceptibility to cephalosporins (<0.0001). However, IS- was not detected in a minority (<10 %) of the isolates which were nonsusceptible to cephalosporins. The acquired OXA-23 enzymes were responsible for nonsusceptibility to carbapenems in our clinical isolates and warrant continuous surveillance to prevent further dissemination of this antibiotic resistance gene. The presence of IS upstream of the was a determinant for cephalosporin resistance. However, the absence of this IS- in some of the isolates may suggest other resistance mechanisms and need further investigation.

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2015-01-01
2019-10-17
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