1887

Abstract

During 2000–2004, 13 strains that were untypable by commercially available antisera were isolated from children <5 years of age with acute diarrhoea in Kolkata. These strains were subsequently identified as provisional serovar 204/96 ( = 3), provisional serovar E23507 ( = 1), provisional serovar I9809-73 ( = 1), provisional serovar 93-119 ( = 1), provisional serovar 88-893 ( = 6) and provisional serovar E16553 ( = 1). In this study, characterization of those provisional serovars of was performed with respect to their antimicrobial resistance, plasmids, virulence genes and PFGE profiles. The drug resistant strains ( = 10) of identified in this study possessed various antibiotic resistance genetic markers like (for chloramphenicol resistance); and (for tetracycline resistance); and (for co-trimoxazole resistance); , and (for streptomycin resistance) and (for ampicillin resistance). Class 1 and/or class 2 integrons were present in eight resistant strains. Three study strains were pan-susceptible. A single mutation in the gene (serine to leucine at codon 83) was present in four quinolone resistant strains. The virulence gene (invasion plasmid antigen H) was uniformly present in all strains in this study, but the (Shiga toxin) and ( enterotoxin 1) genes were absent. Other virulence genes like (invasion associated locus) and ( enterotoxin 2) were occasionally present. A large plasmid of 212 kb and of incompatibility type IncFIIA was present in the majority of the strains ( = 10) and diversity was noticed in the smaller plasmid profiles of these strains even within the same provisional serovars. PFGE profile analysis showed the presence of multiple unrelated clones among the isolates of provisional serovars. To the best of our knowledge, this is the first report on the phenotypic and molecular characterization of provisional serovars of isolates from Kolkata, India.

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2014-12-01
2020-01-27
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