1887

Abstract

Carbapenems are the last-resort antibiotics for the treatment of infections caused by multidrug-resistant Gram-negative bacilli. carbapenemase (KPC) hydrolyses β-lactam antibiotics including the carbapenems. KPCs have been detected in and isolates worldwide associated with transposon Tn commonly located in plasmids. has become an important multidrug-resistant nosocomial pathogen capable of hydrolysing the carbapenem antibiotics. KPC-producing has so far only been reported in Puerto Rico. During a surveillance study, four KPC-producing with identical pulse type were identified in a single institution. The objectives of this study were to characterize the KPC genetic background and the allelic diversity of one of the isolates. Next-generation sequencing and multilocus sequence typing (MLST) were performed. Molecular characterization of the isolate demonstrated in Tn located in the bacterial chromosome within a 26.5 kb DNA fragment, which included a KQ-like element (18.9 kb) very similar to that described previously in a plasmid and a 7.6 kb DNA fragment with 98 % homology to a putative plasmid from strain PY-95. Our data suggested that the 26.5 kb DNA fragment harbouring was integrated in the chromosome by a transposition event mediated by the transposase of IS found in the KQ-like element. MLST showed a novel sequence type, ST250. To our knowledge, this is the first report of the identification of the genetic background of in .

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2014-12-01
2019-09-19
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