1887

Abstract

Intra-familial infection is considered to be one of the main routes of transmission for in Japan. We assessed the genomic profiles of isolates from family members by multi-locus sequence typing (MLST) and identified the original strain infecting the index child. A total of 19 isolates from five families were analysed by MLST using seven housekeeping genes and by random amplification of polymorphic DNA (RAPD)-PCR. Phylogenetic analysis was performed using nucleotide sequences of the seven loci. Two or more different types of strains were indicated in three (K-1, K-2 and K-5) out of five families. Independent genotypes of strains were detected from all members of the other two families suggesting that these strains (K26-28 and K29-33) may be dominant. Mother-to-child transmission of was demonstrated in four out of five families, whilst transmission from father-to-child and sibling-to-sibling were demonstrated in two families and one family, respectively.

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2015-01-01
2020-01-22
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