1887

Abstract

, a worldwide protozoan parasite, is one of the most common causes of diarrhoea in humans and animals. The aim of the present study was to determine species/genotypes in pre-weaned calves in Shaanxi Province using PCR and sequencing based on the small subunit rRNA gene. A total of 258 faecal samples were collected from pre-weaned calves in 19 different farms from six areas in Shaanxi Province, north-western China. infection was detected in 14 of 19 farms (73.7 %), with a total prevalence of 20.2 % (52/258). Both dairy and Qinchuan (beef) cattle were found with infection. Three species, namely ( = 26), ( = 14) and ( = 12), were detected in pre-weaned calves in Shaanxi Province, with (in 12 farms) identified as the most common species on cattle farms. Two additional and previously unknown genotypes, CRTypes III and IV, were observed in the present study. However, the zoonotic species, , was not detected in this study, which suggested a low zoonotic potential in -infected pre-weaned calves in this province.

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2015-01-01
2024-04-19
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References

  1. Amer S., Zidan S., Adamu H., Ye J., Roellig D., Xiao L., Feng Y. 2013; Prevalence and characterization of Cryptosporidium spp. in dairy cattle in Nile River delta provinces, Egypt. Exp Parasitol 135:518–523 [View Article][PubMed]
    [Google Scholar]
  2. Blackburn B. G., Mazurek J. M., Hlavsa M., Park J., Tillapaw M., Parrish M., Salehi E., Franks W., Koch E.& other authors ( 2006; Cryptosporidiosis associated with ozonated apple cider. Emerg Infect Dis 12:684–686 [View Article][PubMed]
    [Google Scholar]
  3. Bouzid M., Hunter P. R., Chalmers R. M., Tyler K. M. 2013; Cryptosporidium pathogenicity and virulence. Clin Microbiol Rev 26:115–134 [View Article][PubMed]
    [Google Scholar]
  4. Brook E. J., Anthony Hart C., French N. P., Christley R. M. 2009; Molecular epidemiology of Cryptosporidium subtypes in cattle in England. Vet J 179:378–382 [View Article][PubMed]
    [Google Scholar]
  5. Chen F., Huang K. 2007; Prevalence and phylogenetic analysis of Cryptosporidium in pigs in eastern China. Zoonoses Public Health 54:393–400 [View Article][PubMed]
    [Google Scholar]
  6. Chen F., Huang K. 2012; Prevalence and molecular characterization of Cryptosporidium spp. in dairy cattle from farms in China. J Vet Sci 13:15–22 [View Article][PubMed]
    [Google Scholar]
  7. Chen F., Qiu H. 2012; Identification and characterization of a Chinese isolate of Cryptosporidium serpentis from dairy cattle. Parasitol Res 111:1785–1791 [View Article][PubMed]
    [Google Scholar]
  8. Chen F., Huang K., Qin S., Zhao Y., Pan C. 2007; Comparison of viability and infectivity of Cryptosporidium parvum oocysts stored in potassium dichromate solution and chlorinated tap water. Vet Parasitol 150:13–17 [View Article][PubMed]
    [Google Scholar]
  9. Coklin T., Farber J., Parrington L., Dixon B. 2007; Prevalence and molecular characterization of Giardia duodenalis and Cryptosporidium spp. in dairy cattle in Ontario, Canada. Vet Parasitol 150:297–305 [View Article][PubMed]
    [Google Scholar]
  10. de Graaf D. C., Vanopdenbosch E., Ortega-Mora L. M., Abbassi H., Peeters J. E. 1999; A review of the importance of cryptosporidiosis in farm animals. Int J Parasitol 29:1269–1287 [View Article][PubMed]
    [Google Scholar]
  11. Fayer R., Santín M., Trout J. M., Greiner E. 2006; Prevalence of species and genotypes of Cryptosporidium found in 1-2-year-old dairy cattle in the eastern United States. Vet Parasitol 135:105–112 [View Article][PubMed]
    [Google Scholar]
  12. Fayer R., Santin M., Trout J. M. 2007; Prevalence of Cryptosporidium species and genotypes in mature dairy cattle on farms in eastern United States compared with younger cattle from the same locations. Vet Parasitol 145:260–266 [View Article][PubMed]
    [Google Scholar]
  13. Fayer R., Santín M., Dargatz D. 2010; Species of Cryptosporidium detected in weaned cattle on cow-calf operations in the United States. Vet Parasitol 170:187–192 [View Article][PubMed]
    [Google Scholar]
  14. Feltus D. C., Giddings C. W., Khaitsa M. L., McEvoy J. M. 2008; High prevalence of Cryptosporidium bovis and the deer-like genotype in calves compared to mature cows in beef cow-calf operations. Vet Parasitol 151:191–195 [View Article][PubMed]
    [Google Scholar]
  15. Feng Y., Ortega Y., He G., Das P., Xu M., Zhang X., Fayer R., Gatei W., Cama V., Xiao L. 2007; Wide geographic distribution of Cryptosporidium bovis and the deer-like genotype in bovines. Vet Parasitol 144:1–9 [View Article][PubMed]
    [Google Scholar]
  16. Feng Y., Karna S. R., Dearen T. K., Singh D. K., Adhikari L. N., Shrestha A., Xiao L. 2012; Common occurrence of a unique Cryptosporidium ryanae variant in zebu cattle and water buffaloes in the buffer zone of the Chitwan National Park, Nepal. Vet Parasitol 185:309–314 [View Article][PubMed]
    [Google Scholar]
  17. Geurden T., Goma F. Y., Siwila J., Phiri I. G., Mwanza A. M., Gabriel S., Claerebout E., Vercruysse J. 2006; Prevalence and genotyping of Cryptosporidium in three cattle husbandry systems in Zambia. Vet Parasitol 138:217–222 [View Article][PubMed]
    [Google Scholar]
  18. Geurden T., Berkvens D., Martens C., Casaert S., Vercruysse J., Claerebout E. 2007; Molecular epidemiology with subtype analysis of Cryptosporidium in calves in Belgium. Parasitology 134:1981–1987 [View Article][PubMed]
    [Google Scholar]
  19. Glaberman S., Moore J. E., Lowery C. J., Chalmers R. M., Sulaiman I., Elwin K., Rooney P. J., Millar B. C., Dooley J. S.& other authors ( 2002; Three drinking-water-associated cryptosporidiosis outbreaks, Northern Ireland. Emerg Infect Dis 8:631–633 [View Article][PubMed]
    [Google Scholar]
  20. Halim N. A., Plutzer J., Bakheit M. A., Karanis P. 2008; First report of Cryptosporidium deer-like genotype in Malaysian cattle. Vet Parasitol 152:325–329 [View Article][PubMed]
    [Google Scholar]
  21. Imre K., Lobo L. M., Matos O., Popescu C., Genchi C., Dărăbuş G. 2011; Molecular characterisation of Cryptosporidium isolates from pre-weaned calves in Romania: is there an actual risk of zoonotic infections?. Vet Parasitol 181:321–324 [View Article][PubMed]
    [Google Scholar]
  22. Jex A. R., Pangasa A., Campbell B. E., Whipp M., Hogg G., Sinclair M. I., Stevens M., Gasser R. B. 2008; Classification of Cryptosporidium species from patients with sporadic cryptosporidiosis by use of sequence-based multilocus analysis following mutation scanning. J Clin Microbiol 46:2252–2262 [View Article][PubMed]
    [Google Scholar]
  23. Keshavarz A., Haghighi A., Athari A., Kazemi B., Abadi A., Nazemalhosseini Mojarad E. 2009; Prevalence and molecular characterization of bovine Cryptosporidium in Qazvin province, Iran. Vet Parasitol 160:316–318 [View Article][PubMed]
    [Google Scholar]
  24. Khan S. M., Debnath C., Pramanik A. K., Xiao L., Nozaki T., Ganguly S. 2010; Molecular characterization and assessment of zoonotic transmission of Cryptosporidium from dairy cattle in West Bengal, India. Vet Parasitol 171:41–47 [View Article][PubMed]
    [Google Scholar]
  25. Langkjaer R. B., Vigre H., Enemark H. L., Maddox-Hyttel C. 2007; Molecular and phylogenetic characterization of Cryptosporidium and Giardia from pigs and cattle in Denmark. Parasitology 134:339–350 [View Article][PubMed]
    [Google Scholar]
  26. Lin Q., Ren W. X., Gao M., Wang X. Y., Cong M. M., Cheng W. Y., Hu B., Li H. M., Yu S. K., Zhao G. H. 2012; Prevalence of the intestinal parasite infection in cattle in Shaanxi province, northwestern China. Afri J Microbiol Res 6:6252–6256
    [Google Scholar]
  27. Maikai B. V., Umoh J. U., Kwaga J. K., Lawal I. A., Maikai V. A., Cama V., Xiao L. 2011; Molecular characterization of Cryptosporidium spp. in native breeds of cattle in Kaduna State, Nigeria. Vet Parasitol 178:241–245 [View Article][PubMed]
    [Google Scholar]
  28. McNabb S. J., Hensel D. M., Welch D. F., Heijbel H., McKee G. L., Istre G. R. 1985; Comparison of sedimentation and flotation techniques for identification of Cryptosporidium sp. oocysts in a large outbreak of human diarrhea. J Clin Microbiol 22:587–589[PubMed]
    [Google Scholar]
  29. Meng Y. 2013; On current situation and sustainable development of large-scale livestock and poultry raising in Shaanxi province. J Northwest A&F Univ (Social Science Edition) 13:88–97 (in Chinese)
    [Google Scholar]
  30. Millard P. S., Gensheimer K. F., Addiss D. G., Sosin D. M., Beckett G. A., Houck-Jankoski A., Hudson A. 1994; An outbreak of cryptosporidiosis from fresh-pressed apple cider. JAMA 272:1592–1596 [View Article][PubMed]
    [Google Scholar]
  31. Murakoshi F., Xiao L., Matsubara R., Sato R., Kato Y., Sasaki T., Fukuda Y., Tada C., Nakai Y. 2012; Molecular characterization of Cryptosporidium spp. in grazing beef cattle in Japan. Vet Parasitol 187:123–128 [View Article][PubMed]
    [Google Scholar]
  32. Nguyen S. T., Fukuda Y., Tada C., Sato R., Duong B., Nguyen D. T., Nakai Y. 2012; Molecular characterization of Cryptosporidium in native beef calves in central Vietnam. Parasitol Res 111:1817–1820 [View Article][PubMed]
    [Google Scholar]
  33. Petry F., Jakobi V., Tessema T. S. 2010; Host immune response to Cryptosporidium parvum infection. Exp Parasitol 126:304–309 [View Article][PubMed]
    [Google Scholar]
  34. Plutzer J., Karanis P. 2007; Genotype and subtype analyses of Cryptosporidium isolates from cattle in Hungary. Vet Parasitol 146:357–362 [View Article][PubMed]
    [Google Scholar]
  35. Quiroz E. S., Bern C., MacArthur J. R., Xiao L., Fletcher M., Arrowood M. J., Shay D. K., Levy M. E., Glass R. I., Lal A. 2000; An outbreak of cryptosporidiosis linked to a foodhandler. J Infect Dis 181:695–700 [View Article][PubMed]
    [Google Scholar]
  36. Rzeżutka A., Kaupke A. 2013; Occurrence and molecular identification of Cryptosporidium species isolated from cattle in Poland. Vet Parasitol 196:301–306 [View Article][PubMed]
    [Google Scholar]
  37. Santín M., Trout J. M., Fayer R. 2008; A longitudinal study of cryptosporidiosis in dairy cattle from birth to 2 years of age. Vet Parasitol 155:15–23 [View Article][PubMed]
    [Google Scholar]
  38. Starkey S. R., Zeigler P. E., Wade S. E., Schaaf S. L., Mohammed H. O. 2006; Factors associated with shedding of Cryptosporidium parvum versus Cryptosporidium bovis among dairy cattle in New York State. J Am Vet Med Assoc 229:1623–1626 [View Article][PubMed]
    [Google Scholar]
  39. Szonyi B., Kang’ethe E. K., Mbae C. K., Kakundi E. M., Kamwati S. K., Mohammed H. O. 2008; First report of Cryptosporidium deer-like genotype in Kenyan cattle. Vet Parasitol 153:172–175 [View Article][PubMed]
    [Google Scholar]
  40. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997; The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882 [View Article][PubMed]
    [Google Scholar]
  41. Thomaz A., Meireles M. V., Soares R. M., Pena H. F., Gennari S. M. 2007; Molecular identification of Cryptosporidium spp. from fecal samples of felines, canines and bovines in the state of São Paulo, Brazil. Vet Parasitol 150:291–296 [View Article][PubMed]
    [Google Scholar]
  42. Thompson H. P., Dooley J. S., Kenny J., McCoy M., Lowery C. J., Moore J. E., Xiao L. 2007; Genotypes and subtypes of Cryptosporidium spp. in neonatal calves in Northern Ireland. Parasitol Res 100:619–624 [View Article][PubMed]
    [Google Scholar]
  43. Tzipori S., Widmer G. 2008; A hundred-year retrospective on cryptosporidiosis. Trends Parasitol 24:184–189 [View Article][PubMed]
    [Google Scholar]
  44. Wang R., Ma G., Zhao J., Lu Q., Wang H., Zhang L., Jian F., Ning C., Xiao L. 2011a; Cryptosporidium andersoni is the predominant species in post-weaned and adult dairy cattle in China. Parasitol Int 60:1–4 [View Article][PubMed]
    [Google Scholar]
  45. Wang R., Wang H., Sun Y., Zhang L., Jian F., Qi M., Ning C., Xiao L. 2011b; Characteristics of Cryptosporidium transmission in preweaned dairy cattle in Henan, China. J Clin Microbiol 49:1077–1082 [View Article][PubMed]
    [Google Scholar]
  46. Xiao L. 2010; Molecular epidemiology of cryptosporidiosis: an update. Exp Parasitol 124:80–89 [View Article][PubMed]
    [Google Scholar]
  47. Xiao L., Feng Y. 2008; Zoonotic cryptosporidiosis. FEMS Immunol Med Microbiol 52:309–323 [View Article][PubMed]
    [Google Scholar]
  48. Xiao L., Escalante L., Yang C., Sulaiman I., Escalante A. A., Montali R. J., Fayer R., Lal A. A. 1999; Phylogenetic analysis of Cryptosporidium parasites based on the small-subunit rRNA gene locus. Appl Environ Microbiol 65:1578–1583[PubMed]
    [Google Scholar]
  49. Zhang W., Wang R., Yang F., Zhang L., Cao J., Zhang X., Ling H., Liu A., Shen Y. 2013; Distribution and genetic characterizations of Cryptosporidium spp. in pre-weaned dairy calves in Northeastern China’s Heilongjiang Province. PLoS ONE 8:e54857 [View Article][PubMed]
    [Google Scholar]
  50. Zhao G. H., Ren W. X., Gao M., Bian Q. Q., Hu B., Cong M. M., Lin Q., Wang R. J., Qi M.& other authors ( 2013; Genotyping Cryptosporidium andersoni in cattle in Shaanxi Province, Northwestern China. PLoS ONE 8:e60112 [View Article][PubMed]
    [Google Scholar]
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