1887

Abstract

The purpose of the present study was to identify the clonal characteristics and gene diversity of ciprofloxacin-resistant meningococcal strains in China. One hundred and forty-one ciprofloxacin-resistant and 103 ciprofloxacin-susceptible meningococcal strains were selected for multilocus sequence typing. Of these, 54 ciprofloxacin-resistant and 42 ciprofloxacin-susceptible strains were selected for gene sequencing. Of the three clonal complexes prevalent in China, serogroup A of ST-5 complex (CC5) and serogroup C/B strains of CC4821 had a high proportion of ciprofloxacin resistance, whereas CC11 serogroup W strains were all susceptible. Nucleotide and amino acid sequences of the gene among ciprofloxacin-resistant strains showed more diversity than those among ciprofloxacin-susceptible strains. All ciprofloxacin-resistant strains had a T91I mutation and the ciprofloxacin-susceptible strains had no T91I mutation. Phylogenetic analysis showed that the gene sequences of CC4821 serogroup B/C strains, CC11 serogroup W, CC1 serogroup A, ciprofloxacin-susceptible CC5 serogroup A and reference strains had high similarity. By contrast, the ciprofloxacin-resistant CC5 serogroup A strains had a highly conserved gene sequence which was different (94.8 % similarity) from that in the above strains. The results of our investigation showed that the high proportion of ciprofloxacin resistance in is associated with certain sequence types (STs) or clonal complexes (CCs). The prevalence of certain CCs with a high proportion of ciprofloxacin resistance can facilitate the spread of ciprofloxacin resistance.

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2014-11-01
2019-11-12
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