1887

Abstract

In order to investigate the prevalence of fosfomycin-resistance ( determinants in , clinical strains were collected from hospitals throughout China between January 2008 and December 2009. Antimicrobial susceptibility testing was performed, after which the genes in all isolates and genes in vancomycin-resistant isolates were characterized by PCR and sequencing. Conjugation experiments were carried out with -positive , DNA fragments flanking the gene were sequenced and the genetic environment of was analysed. Fosfomycin-resistant (FREF) strains were characterized further by multilocus sequence typing (MLST) and PFGE. Among 145 clinical isolates, 10 were resistant to fosfomycin with MICs >1024 mg l including six vancomycin-resistant strains of which five were -positive and the sixth -positive. All ten FREF strains harboured the gene. Fosfomycin resistance and could be transferred by conjugation from nine isolates. The and genes were located in a circular DNA intermediate in all FREF strains and reversely inserted into transposon Tn1546 in four -positive FREF isolates. Ten different PFGE types and seven MLST types were found among the ten positive isolates, while all strains belonged to the common clonal complex CC17. In conclusion, the transferable fosfomycin-resistance determinant plays an important role in resistance to fosfomycin in China.

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2014-11-01
2019-11-13
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