1887

Abstract

In order to investigate the prevalence of fosfomycin-resistance ( determinants in , clinical strains were collected from hospitals throughout China between January 2008 and December 2009. Antimicrobial susceptibility testing was performed, after which the genes in all isolates and genes in vancomycin-resistant isolates were characterized by PCR and sequencing. Conjugation experiments were carried out with -positive , DNA fragments flanking the gene were sequenced and the genetic environment of was analysed. Fosfomycin-resistant (FREF) strains were characterized further by multilocus sequence typing (MLST) and PFGE. Among 145 clinical isolates, 10 were resistant to fosfomycin with MICs >1024 mg l including six vancomycin-resistant strains of which five were -positive and the sixth -positive. All ten FREF strains harboured the gene. Fosfomycin resistance and could be transferred by conjugation from nine isolates. The and genes were located in a circular DNA intermediate in all FREF strains and reversely inserted into transposon Tn1546 in four -positive FREF isolates. Ten different PFGE types and seven MLST types were found among the ten positive isolates, while all strains belonged to the common clonal complex CC17. In conclusion, the transferable fosfomycin-resistance determinant plays an important role in resistance to fosfomycin in China.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.077701-0
2014-11-01
2024-11-08
Loading full text...

Full text loading...

/deliver/fulltext/jmm/63/11/1484.html?itemId=/content/journal/jmm/10.1099/jmm.0.077701-0&mimeType=html&fmt=ahah

References

  1. Allerberger F., Klare I. 1999; In vitro activity of fosfomycin against vancomycin-resistant enterococci. J Antimicrob Chemother 43:211–217 [View Article][PubMed]
    [Google Scholar]
  2. Barbier N., Saulnier P., Chachaty E., Dumontier S., Andremont A. 1996; Random amplified polymorphic DNA typing versus pulsed-field gel electrophoresis for epidemiological typing of vancomycin-resistant enterococci. J Clin Microbiol 34:1096–1099[PubMed]
    [Google Scholar]
  3. Butcu M., Akcay S. S., Aksaray S., Calisici G., Inan A. S., Engin D. O. 2011; In vitro susceptibility of enterococci strains isolated from urine samples to fosfomycin and other antibiotics. J Infect Chemother 17:575–578 [View Article][PubMed]
    [Google Scholar]
  4. Cao M., Bernat B. A., Wang Z., Armstrong R. N., Helmann J. D. 2001; FosB, a cysteine-dependent fosfomycin resistance protein under the control of sigma(W), an extracytoplasmic-function sigma factor in Bacillus subtilis.. J Bacteriol 183:2380–2383 [View Article][PubMed]
    [Google Scholar]
  5. Carriço J. A., Pinto F. R., Simas C., Nunes S., Sousa N. G., Frazão N., de Lencastre H., Almeida J. S. 2005; Assessment of band-based similarity coefficients for automatic type and subtype classification of microbial isolates analyzed by pulsed-field gel electrophoresis. J Clin Microbiol 43:5483–5490 [View Article][PubMed]
    [Google Scholar]
  6. CLSI 2010; Performance Standards for Antimicrobial Susceptibility Testing; 20th Informational Supplement M100–S20. Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  7. d’Azevedo P. A., Dias C. A., Teixeira L. M. 2006; Genetic diversity and antimicrobial resistance of enterococcal isolates from Southern region of Brazil. Rev Inst Med Trop Sao Paulo 48:11–16 [View Article][PubMed]
    [Google Scholar]
  8. Feil E. J., Li B. C., Aanensen D. M., Hanage W. P., Spratt B. G. 2004; eBURST: inferring patterns of evolutionary descent among clusters of related bacterial genotypes from multilocus sequence typing data. J Bacteriol 186:1518–1530 [View Article][PubMed]
    [Google Scholar]
  9. Franke A. E., Clewell D. B. 1981; Evidence for a chromosome-borne resistance transposon (Tn916) in Streptococcus faecalis that is capable of “conjugal” transfer in the absence of a conjugative plasmid. J Bacteriol 145:494–502[PubMed]
    [Google Scholar]
  10. Homan W. L., Tribe D., Poznanski S., Li M., Hogg G., Spalburg E., Van Embden J. D., Willems R. J. 2002; Multilocus sequence typing scheme for Enterococcus faecium.. J Clin Microbiol 40:1963–1971 [View Article][PubMed]
    [Google Scholar]
  11. Kahan F. M., Kahan J. S., Cassidy P. J., Kropp H. 1974; The mechanism of action of fosfomycin (phosphonomycin). Ann N Y Acad Sci 235:364–386 [View Article][PubMed]
    [Google Scholar]
  12. Lamers A. P., Keithly M. E., Kim K., Cook P. D., Stec D. F., Hines K. M., Sulikowski G. A., Armstrong R. N. 2012; Synthesis of bacillithiol and the catalytic selectivity of FosB-type fosfomycin resistance proteins. Org Lett 14:5207–5209 [View Article][PubMed]
    [Google Scholar]
  13. Michalopoulos A. S., Livaditis I. G., Gougoutas V. 2011; The revival of fosfomycin. Int J Infect Dis 15:e732–e739 [View Article][PubMed]
    [Google Scholar]
  14. Perri M. B., Hershberger E., Ionescu M., Lauter C., Zervos M. J. 2002; In vitro susceptibility of vancomycin-resistant enterococci (VRE) to fosfomycin. Diagn Microbiol Infect Dis 42:269–271 [View Article][PubMed]
    [Google Scholar]
  15. Qu T. T., Yang Q., Shen P., Wei Z. Q., Yu Y. S. 2012; Novel vancomycin-resistance transposon, plasmid replicon types, and virulence factors of vancomycin-resistant enterococci in Zhejiang, China. Microb Drug Resist 18:183–188 [View Article][PubMed]
    [Google Scholar]
  16. Rigsby R. E., Fillgrove K. L., Beihoffer L. A., Armstrong R. N. 2005; Fosfomycin resistance proteins: a nexus of glutathione transferases and epoxide hydrolases in a metalloenzyme superfamily. Methods Enzymol 401:367–379 [View Article][PubMed]
    [Google Scholar]
  17. Sahni R. D., Balaji V., Varghese R., John J., Tansarli G. S., Falagas M. E. 2013; Evaluation of fosfomycin activity against uropathogens in a fosfomycin-naive population in South India: a prospective study. Future Microbiol 8:675–680 [View Article][PubMed]
    [Google Scholar]
  18. Sun H., Wang H., Xu Y., Jones R. N., Costello A. J., Liu Y., Li G., Chen M., Mendes R. E. 2012; Molecular characterization of vancomycin-resistant Enterococcus spp. clinical isolates recovered from hospitalized patients among several medical institutions in China. Diagn Microbiol Infect Dis 74:399–403 [View Article][PubMed]
    [Google Scholar]
  19. Wang F., Zhu D., Hu F., Ruan F., Ni Y., Sun J., Xu Y., Sun H., Hu Y. other authors 2008; CHINET 2007 surveillance of bacterial resistance in China. Chinese Journal of Infection and Chemotherapy 8:325–332 (in Chinese)
    [Google Scholar]
  20. Willems R. J., Top J., van Santen M., Robinson D. A., Coque T. M., Baquero F., Grundmann H., Bonten M. J. 2005; Global spread of vancomycin-resistant Enterococcus faecium from distinct nosocomial genetic complex. Emerg Infect Dis 11:821–828 [View Article][PubMed]
    [Google Scholar]
  21. Xu X., Lin D., Yan G., Ye X., Wu S., Guo Y., Zhu D., Hu F., Zhang Y.& other authors ( 2010; vanM, a new glycopeptide resistance gene cluster found in Enterococcus faecium.. Antimicrob Agents Chemother 54:4643–4647 [View Article][PubMed]
    [Google Scholar]
  22. Xu H. T., Tian R., Chen D. K., Xiao F., Nie Z. Y., Hu Y. J., Zhang X. Z., Li J. M. 2011; Nosocomial spread of hospital-adapted CC17 vancomycin-resistant Enterococcus faecium in a tertiary-care hospital of Beijing, China. Chin Med J (Engl) 124:498–503[PubMed]
    [Google Scholar]
  23. Xu X., Chen C., Lin D., Guo Q., Hu F., Zhu D., Li G., Wang M. 2013; The fosfomycin resistance gene fosB3 is located on a transferable, extrachromosomal circular intermediate in clinical Enterococcus faecium isolates. PLoS ONE 8:e78106 [View Article][PubMed]
    [Google Scholar]
  24. Zilhao R., Courvalin P. 1990; Nucleotide sequence of the fosB gene conferring fosfomycin resistance in Staphylococcus epidermidis.. FEMS Microbiol Lett 56:267–272[PubMed]
    [Google Scholar]
/content/journal/jmm/10.1099/jmm.0.077701-0
Loading
/content/journal/jmm/10.1099/jmm.0.077701-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error