RT Journal Article SR Electronic(1) A1 Okuda, Masumi A1 Osaki, Takako A1 Kikuchi, Shogo A1 Ueda, Junko A1 Lin, Yingsong A1 Yonezawa, Hideo A1 Maekawa, Kohei A1 Hojo, Fuhito A1 Kamiya, Shigeru A1 Fukuda, YoshihiroYR 2014 T1 Evaluation of a stool antigen test using a mAb for native catalase for diagnosis of Helicobacter pylori infection in children and adults JF Journal of Medical Microbiology, VO 63 IS 12 SP 1621 OP 1625 DO https://doi.org/10.1099/jmm.0.077370-0 PB Microbiology Society, SN 1473-5644, AB Non-invasive diagnosis of Helicobacter pylori infection is important not only for screening of infection but also for epidemiological studies. Stool antigen tests are non-invasive and are convenient to identify H. pylori infection, particularly in children. We evaluated the stool antigen test, which uses a mAb for native catalase of H. pylori developed in Japan. A total of 151 stool samples were collected from participants (52 children and 99 adults) of the Sasayama Cohort Study and stored between −30 and −80 °C. The stool antigen test used was Testmate pylori antigen (TPAg), and was performed according to the manufacturer’s instructions. Furthermore, we conducted a quantitative real-time PCR test and compared the PCR results with those of the TPAg test. When compared with the results in real-time PCR, the sensitivity of TPAg was 89.5 % overall, 82.7 % for children and 92.4 % for adults, and the specificity was 100 %. The accuracy was 93.4 % overall, 90.4 % for children and 94.9 % for adults, and there was no significant difference in the accuracy of TPAg between children and adults. Five of 28 children (18 %) and five of 38 adults (13 %) were PCR positive with negative TPAg results. Four of five children with positive PCR and negative TPAg results were given a 13C-urea breath test and all four children tested negative. No significant correlation was observed between the TPAg results and DNA numbers of H. pylori in faeces among children or adults. A stool antigen test (TPAg) using a mAb for native catalase is useful for diagnosis of H. pylori in children and adults. Additionally, this test has particularly high specificity., UL https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.077370-0