1887

Abstract

The predisposition of patients with cystic fibrosis (CF) for recurrent pulmonary infections can result in poor prognosis of the disease. Although the clinical significance in CF of micro-organisms, such as , and , is well established, the implication of uncommon glucose non-fermenting Gram-negative bacilli (UGNF-GNB) in respiratory samples from CF patients is still unclear. Because of limitations of traditional methods used in most clinical laboratories, the accurate identification of these microbes is a challenge. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) is an alternative tool for efficient identification of bacteria. This was a retrospective study to evaluate different identification methods in a collection of UGNF-GNB isolated from children with CF during a period of three years. The performance of MALDI-TOF was compared to that of 16S rDNA gene sequencing and to a conventional and automated phenotypic identification. The discriminatory power of MALDI-TOF (75.0 % agreement) was superior to automated techniques (67.1 % agreement) and to conventional phenotypical identification (50.0 % agreement). MALDI-TOF also demonstrated high accuracy in identifying , and , but had limited utility in identifying spp. and some species of and (other than ). Although MALDI-TOF identified only 75 % of the isolates in comparison with 16S rDNA gene sequencing, the prompt identification and high discriminatory power exhibited by MALDI-TOF make it a useful tool for the characterization of micro-organisms that are difficult to identify using routine methods.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.076869-0
2014-09-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jmm/63/9/1148.html?itemId=/content/journal/jmm/10.1099/jmm.0.076869-0&mimeType=html&fmt=ahah

References

  1. Álvarez-Buylla A., Culebras E., Picazo J. J. 2012; Identification of Acinetobacter species: is Bruker biotyper MALDI-TOF mass spectrometry a good alternative to molecular techniques?. Infect Genet Evol 12:345–349 [View Article][PubMed]
    [Google Scholar]
  2. Amin R., Waters V. 2014; Antibiotic treatment for Stenotrophomonas maltophilia in people with cystic fibrosis. Cochrane Database Syst Rev 4:CD009249 [View Article][PubMed]
    [Google Scholar]
  3. Bendinelli M., Friedman H., Bergogne-Bérézin E.(editors) 2008 Acinetobacter: Biology and Pathogenesis New York, NY: Springer; [View Article]
    [Google Scholar]
  4. Bosshard P. P., Zbinden R., Abels S., Böddinghaus B., Altwegg M., Böttger E. C. 2006; 16S rRNA gene sequencing versus the API 20 NE system and the VITEK 2 ID-GNB card for identification of nonfermenting Gram-negative bacteria in the clinical laboratory. J Clin Microbiol 44:1359–1366 [View Article][PubMed]
    [Google Scholar]
  5. Calderón G., García E., Rojas P., García E., Rosso M., Losada A. 2011; Chryseobacterium indologenes infection in a newborn: a case report. J Med Case Reports 5:10 [View Article][PubMed]
    [Google Scholar]
  6. Coenye T., Goris J., Spilker T., Vandamme P., LiPuma J. J. 2002; Characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of Inquilinus limosus gen. nov., sp. nov. J Clin Microbiol 40:2062–2069 [View Article][PubMed]
    [Google Scholar]
  7. Costello A., Herbert G., Fabunmi L., Schaffer K., Kavanagh K. A., Caraher E. M., Callaghan M., McClean S. 2011; Virulence of an emerging respiratory pathogen, genus Pandoraea, in vivo and its interactions with lung epithelial cells. J Med Microbiol 60:289–299 [View Article][PubMed]
    [Google Scholar]
  8. Desai A. P., Stanley T., Atuan M., McKey J., Lipuma J. J., Rogers B., Jerris R. 2012; Use of matrix assisted laser desorption ionisation-time of flight mass spectrometry in a paediatric clinical laboratory for identification of bacteria commonly isolated from cystic fibrosis patients. J Clin Pathol 65:835–838 [View Article][PubMed]
    [Google Scholar]
  9. Fernández-Olmos A., Morosini M. I., Lamas A., García-Castillo M., García-García L., Cantón R., Máiz L. 2012a; Clinical and microbiological features of a cystic fibrosis patient chronically colonized with Pandoraea sputorum identified by combining 16S rRNA sequencing and matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 50:1096–1098 [View Article][PubMed]
    [Google Scholar]
  10. Fernández-Olmos A., García-Castillo M., Morosini M. I., Lamas A., Máiz L., Cantón R. 2012b; MALDI-TOF MS improves routine identification of non-fermenting Gram negative isolates from cystic fibrosis patients. J Cyst Fibros 11:59–62 [View Article][PubMed]
    [Google Scholar]
  11. Hansen C. R., Pressler T., Nielsen K. G., Jensen P. Ø., Bjarnsholt T., Høiby N. 2010; Inflammation in Achromobacter xylosoxidans infected cystic fibrosis patients. J Cyst Fibros 9:51–58 [View Article][PubMed]
    [Google Scholar]
  12. Lambiase A., Del Pezzo M., Raia V., Sepe A., Ferri P., Rossano F. 2007; Chryseobacterium respiratory tract infections in patients with cystic fibrosis. J Infect 55:518–523 [View Article][PubMed]
    [Google Scholar]
  13. Lambiase A., Catania M. R., Del Pezzo M., Rossano F., Terlizzi V., Sepe A., Raia V. 2011; Achromobacter xylosoxidans respiratory tract infection in cystic fibrosis patients. Eur J Clin Microbiol Infect Dis 30:973–980 [View Article][PubMed]
    [Google Scholar]
  14. Lay J. O. Jr 2001; MALDI-TOF mass spectrometry of bacteria. Mass Spectrom Rev 20:172–194 [View Article][PubMed]
    [Google Scholar]
  15. Marko D. C., Saffert R. T., Cunningham S. A., Hyman J., Walsh J., Arbefeville S., Howard W., Pruessner J., Safwat N.& other authors ( 2012; Evaluation of the Bruker Biotyper and Vitek MS matrix-assisted laser desorption ionization-time of flight mass spectrometry systems for identification of nonfermenting Gram-negative bacilli isolated from cultures from cystic fibrosis patients. J Clin Microbiol 50:2034–2039 [View Article][PubMed]
    [Google Scholar]
  16. Martínez-Lamas L., Rabade Castedo C., Martín Romero Domínguez M., Barbeito Castiñeiras G., Palacios Bartolomé A., Pérez Del Molino Bernal M. L. 2011; [Pandoraea sputorum colonization in a patient with cystic fibrosis]. Arch Bronconeumol 47:571–574 (in Spanish) [View Article][PubMed]
    [Google Scholar]
  17. Paschoal I. A., Pereira M. C. 2010 Fibrose Cística. Sâo Caetano do Sul: Yendis;
    [Google Scholar]
  18. Rettinger A., Krupka I., Grünwald K., Dyachenko V., Fingerle V., Konrad R., Raschel H., Busch U., Sing A.& other authors ( 2012; Leptospira spp. strain identification by MALDI TOF MS is an equivalent tool to 16S rRNA gene sequencing and multi locus sequence typing (MLST). BMC Microbiol 12:185 [View Article][PubMed]
    [Google Scholar]
  19. Richter S. S., Sercia L., Branda J. A., Burnham C.-A. D., Bythrow M., Ferraro M. J., Garner O. B., Ginocchio C. C., Jennemann R.& other authors ( 2013; Identification of Enterobacteriaceae by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using the VITEK MS system. Eur J Clin Microbiol Infect Dis 32:1571–1578 [View Article][PubMed]
    [Google Scholar]
  20. Ridderberg W., Wang M., Nørskov-Lauritsen N. 2012; Multilocus sequence analysis of isolates of Achromobacter from patients with cystic fibrosis reveals infecting species other than Achromobacter xylosoxidans. J Clin Microbiol 50:2688–2694 [View Article][PubMed]
    [Google Scholar]
  21. Sakurada Z. A. 2008; [Chryseobacterium indologenes]. Rev Chilena Infectol 25:446 (in Spanish) [View Article][PubMed]
    [Google Scholar]
  22. Samonis G., Karageorgopoulos D. E., Maraki S., Levis P., Dimopoulou D., Spernovasilis N. A., Kofteridis D. P., Falagas M. E. 2012; Stenotrophomonas maltophilia infections in a general hospital: patient characteristics, antimicrobial susceptibility, and treatment outcome. PLoS ONE 7:e37375 [View Article][PubMed]
    [Google Scholar]
  23. Schreckenberger P., Daneshvar M., Weyant R., Hollis D. 2003; Acinetobacter, Achromobacter, Chryseobacterium, Moraxella and other nonfermentative Gram negative rods. In Manual of Clinical Microbiology, 8th edn. p. 1212 Edited by Murray P., Baron E. , Jorgensen J., Pfaller M., Yolken R. Washington, DC: ASM Press;
    [Google Scholar]
  24. Tan K., Conway S. P., Brownlee K. G., Etherington C., Peckham D. G. 2002; Alcaligenes infection in cystic fibrosis. Pediatr Pulmonol 34:101–104 [View Article][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.076869-0
Loading
/content/journal/jmm/10.1099/jmm.0.076869-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error