1887

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Volume 63, Issue 12

Interactions of uroseptic with renal (A-498) and gastrointestinal (HT-29) cell lines Free

Abstract

We investigated the ability of isolated from septic patients with urinary tract infection (UTI) to translocate through the gastrointestinal (GI) tract of the same patients using cell-culture models. Forty-seven hospitalized patients with urosepsis were included in this study. was isolated from their urine and blood (total 94 isolates) and investigated for genetic relatedness and interaction with the cell lines A-498 and HT-29. An initial comparison of the strains isolated from urine and blood showed that 44 out of 47 patients (94 %) had identical strains in their blood and urine. The blood isolates adhered to both cell lines, although their rate of adherence to A-498 cells was significantly higher than that to HT-29 cells (5.8±3.8 per cell vs 2.8±1.9; <0.0001). The rate of translocation in A-498 cells was also significantly higher after 120 min (8.7×10 vs 2.9×10;  = 0.0006). Three non-identical blood isolates were unable to translocate in HT-29 cells, indicating that host immune factors might be more important than bacterial ability to translocate the GI epithelium in these patients. Our data showed that blood isolates from uroseptic patients are able to adhere to and translocate through both cell lines. This suggests that in patients with UTI may translocate from either the GI tract or the urinary tract, hence questioning the assumption that the urinary tract is the only source of septicaemia in these patients.

  • Received:
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© 2014 The Authors
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2014-12-01
2024-04-25
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Interactions of uroseptic Escherichia coli with renal (A-498) and gastrointestinal (HT-29) cell lines
J. Med. Microbiol. 63, 1575 (2014); https://doi.org/10.1099/jmm.0.076562-0
/content/journal/jmm/10.1099/jmm.0.076562-0
/content/journal/jmm/10.1099/jmm.0.076562-0
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