This study aimed to identify strains of the Candida parapsilosis complex isolated from animals, as well as to assess their in vitro antifungal susceptibility profile and in vitro production of virulence attributes. We used 28 isolates of C. parapsilosis sensu lato recovered from clinically healthy animals. The strains were characterized phenotypically, followed by molecular identification of the species through PCR-restriction enzyme analysis. The susceptibility of the strains to amphotericin B, itraconazole, voriconazole, fluconazole and caspofungin was assessed through broth microdilution. Additionally, the ability of the strains to produce biofilm, phospholipases and proteases was analysed. Molecular analysis showed 13 C. parapsilosis sensu stricto, 10 Candida orthopsilosis and five Candida metapsilosis strains. In vitro resistance to fluconazole was observed in three strains of C. parapsilosis sensu stricto and two C. metapsilosis. All tested strains were able to form biofilms and 23/28 isolates presented protease production, whilst none was able to produce phospholipases. Our study showed that C. parapsilosis sensu stricto and C. orthopsilosis are the most common species of the C. parapsilosis species complex and that these cryptic species present no significant phenotypical differences.
BrilhanteR. S. N.,
Castelo-BrancoD. S. C. M.,
SoaresG. D.,
Astete-MedranoD. J.,
MonteiroA. J.,
CordeiroR. A.,
SidrimJ. J. C.,
RochaM. F.2010; Characterization of the gastrointestinal yeast microbiota of cockatiels (Nymphicus hollandicus): a potential hazard to human health. J Med Microbiol 59:718–723 [View Article][PubMed]
BrilhanteR. S. N.,
PaivaM. A. N.,
SampaioC. M. S.,
TeixeiraC. E. C.,
Castelo-BrancoD. S. C. M.,
LeiteJ. J. G.,
MoreiraC. A.,
SilvaL. P.,
CordeiroR. A.other authors2011; Yeasts from Macrobrachium amazonicum: a focus on antifungal susceptibility and virulence factors of Candida spp. FEMS Microbiol Ecol 76:268–277 [View Article][PubMed]
BritoE. H. S.,
FontenelleR. O. S.,
BrilhanteR. S. N.,
CordeiroR. A.,
MonteiroA. J.,
SidrimJ. J. C.,
RochaM. F. G.2009; The anatomical distribution and antimicrobial susceptibility of yeast species isolated from healthy dogs. Vet J 182:320–326[CrossRef]
CantónE.,
Espinel-IngroffA,
PemánJ.,
del CastilloL.2010; In vitro fungicidal activities of echinocandins against Candida metapsilosis, C. orthopsilosis, and C. parapsilosis evaluated by time-kill studies. Antimicrob Agents Chemother 54:2194–2197 [View Article][PubMed]
CantónE.,
PemánJ.,
QuindósG.,
ErasoE.,
Miranda-ZapicoI.,
ÁlvarezM.,
MerinoP.,
Campos-HerreroI.,
MarcoF.other authors2011; Prospective multicenter study of the epidemiology, molecular identification, and antifungal susceptibility of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis isolated from patients with candidemia. Antimicrob Agents Chemother 55:5590–5596 [View Article][PubMed]
FengX.,
LingB.,
YangG.,
YuX.,
RenD.,
YaoZ.2012; Prevalence and distribution profiles of Candida parapsilosis, Candida orthopsilosis and Candida metapsilosis responsible for superficial candidiasis in a Chinese university hospital. Mycopathologia 173:229–234 [View Article][PubMed]
GómezJ.,
García-VázquezE.,
EspinosaC.,
RuizJ.,
CanterasM.,
Hernández-TorresA.,
BañosV.,
HerreroJ. A.,
ValdésM.2009; Nosocomial candidemia at a general hospital: the change of epidemiological and clinical characteristics. A comparative study of 2 cohorts (1993–1998 versus 2002–2005). Rev Iberoam Micol 26:184–188 [View Article][PubMed]
PfallerM. A.,
AndesD.,
DiekemaD. J.,
Espinel-IngroffA.,
SheehanD.CLSI Subcommittee for Antifungal Susceptibility Testing2010; Wild-type MIC distributions, epidemiological cutoff values and species-specific clinical breakpoints for fluconazole and Candida: time for harmonization of CLSI and EUCAST broth microdilution methods. Drug Resist Updat 13:180–195 [View Article][PubMed]
SidrimJ. J. C.,
MaiaD. C.,
BrilhanteR. S. N.,
SoaresG. D. P.,
CordeiroR. A.,
MonteiroA. J.,
RochaM. F. G.2010; Candida species isolated from the gastrointestinal tract of cockatiels (Nymphicus hollandicus): in vitro antifungal susceptibility profile and phospholipase activity. Vet Microbiol 145:324–328 [View Article][PubMed]
SpampinatoC.,
LeonardiD.2013; Candida infections, causes, targets, and resistance mechanisms: traditional and alternative antifungal agents. BioMed Res Int2013204237.
TavantiA.,
DavidsonA. D.,
GowN. A.,
MaidenM. C. J.,
OddsF. C.2005; Candida orthopsilosis and Candida metapsilosis spp. nov. to replace Candida parapsilosis groups II and III. J Clin Microbiol 43:284–292 [View Article][PubMed]