1887

Abstract

This study aimed to identify strains of the complex isolated from animals, as well as to assess their antifungal susceptibility profile and production of virulence attributes. We used 28 isolates of recovered from clinically healthy animals. The strains were characterized phenotypically, followed by molecular identification of the species through PCR-restriction enzyme analysis. The susceptibility of the strains to amphotericin B, itraconazole, voriconazole, fluconazole and caspofungin was assessed through broth microdilution. Additionally, the ability of the strains to produce biofilm, phospholipases and proteases was analysed. Molecular analysis showed 13 , 10 and five strains. resistance to fluconazole was observed in three strains of and two . . All tested strains were able to form biofilms and 23/28 isolates presented protease production, whilst none was able to produce phospholipases. Our study showed that . and . are the most common species of the . species complex and that these cryptic species present no significant phenotypical differences.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.076216-0
2014-11-01
2019-11-17
Loading full text...

Full text loading...

/deliver/fulltext/jmm/63/11/1568.html?itemId=/content/journal/jmm/10.1099/jmm.0.076216-0&mimeType=html&fmt=ahah

References

  1. Brilhante R. S. N. , Castelo-Branco D. S. C. M. , Soares G. D. , Astete-Medrano D. J. , Monteiro A. J. , Cordeiro R. A. , Sidrim J. J. C. , Rocha M. F. . ( 2010; ). Characterization of the gastrointestinal yeast microbiota of cockatiels (Nymphicus hollandicus): a potential hazard to human health. . J Med Microbiol 59:, 718–723. [CrossRef] [PubMed]
    [Google Scholar]
  2. Brilhante R. S. N. , Paiva M. A. N. , Sampaio C. M. S. , Teixeira C. E. C. , Castelo-Branco D. S. C. M. , Leite J. J. G. , Moreira C. A. , Silva L. P. , Cordeiro R. A. . & other authors ( 2011; ). Yeasts from Macrobrachium amazonicum: a focus on antifungal susceptibility and virulence factors of Candida spp. . FEMS Microbiol Ecol 76:, 268–277. [CrossRef] [PubMed]
    [Google Scholar]
  3. Brito E. H. S. , Fontenelle R. O. S. , Brilhante R. S. N. , Cordeiro R. A. , Monteiro A. J. , Sidrim J. J. C. , Rocha M. F. G. . ( 2009; ). The anatomical distribution and antimicrobial susceptibility of yeast species isolated from healthy dogs. . Vet J 182:, 320–326. [CrossRef]
    [Google Scholar]
  4. Cantón E. , Espinel-Ingroff A , Pemán J. , del Castillo L. . ( 2010; ). In vitro fungicidal activities of echinocandins against Candida metapsilosis, C. orthopsilosis, and C. parapsilosis evaluated by time-kill studies. . Antimicrob Agents Chemother 54:, 2194–2197. [CrossRef] [PubMed]
    [Google Scholar]
  5. Cantón E. , Pemán J. , Quindós G. , Eraso E. , Miranda-Zapico I. , Álvarez M. , Merino P. , Campos-Herrero I. , Marco F. . & other authors ( 2011; ). Prospective multicenter study of the epidemiology, molecular identification, and antifungal susceptibility of Candida parapsilosis, Candida orthopsilosis, and Candida metapsilosis isolated from patients with candidemia. . Antimicrob Agents Chemother 55:, 5590–5596. [CrossRef] [PubMed]
    [Google Scholar]
  6. CLSI ( 2008; ). Reference Method for Broth Dilution Antifungal Susceptibility Testing of Yeasts; Approved Standard, 3rd edn, M27-A3. . Wayne, PA:: Clinical and Laboratory Standards Institute;.
  7. Feng X. , Ling B. , Yang G. , Yu X. , Ren D. , Yao Z. . ( 2012; ). Prevalence and distribution profiles of Candida parapsilosis, Candida orthopsilosis and Candida metapsilosis responsible for superficial candidiasis in a Chinese university hospital. . Mycopathologia 173:, 229–234. [CrossRef] [PubMed]
    [Google Scholar]
  8. Gómez J. , García-Vázquez E. , Espinosa C. , Ruiz J. , Canteras M. , Hernández-Torres A. , Baños V. , Herrero J. A. , Valdés M. . ( 2009; ). Nosocomial candidemia at a general hospital: the change of epidemiological and clinical characteristics. A comparative study of 2 cohorts (1993–1998 versus 2002–2005). . Rev Iberoam Micol 26:, 184–188. [CrossRef] [PubMed]
    [Google Scholar]
  9. Peeters E. , Nelis H. J. , Coenye T. . ( 2008; ). Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. . J Microbiol Methods 72:, 157–165. [CrossRef] [PubMed]
    [Google Scholar]
  10. Pfaller M. A. , Andes D. , Diekema D. J. , Espinel-Ingroff A. , Sheehan D. . CLSI Subcommittee for Antifungal Susceptibility Testing ( 2010; ). Wild-type MIC distributions, epidemiological cutoff values and species-specific clinical breakpoints for fluconazole and Candida: time for harmonization of CLSI and EUCAST broth microdilution methods. . Drug Resist Updat 13:, 180–195. [CrossRef] [PubMed]
    [Google Scholar]
  11. Price M. F. , Wilkinson I. D. , Gentry L. O. . ( 1982; ). Plate method for detection of phospholipase activity in Candida albicans . . Sabouraudia 20:, 7–14. [CrossRef] [PubMed]
    [Google Scholar]
  12. Ravi S. , Pierce C. , Witt C. , Wormley F. L. Jr . ( 2009; ). Biofilm formation by Cryptococcus neoformans under distinct environmental conditions. . Mycopathologia 167:, 307–314. [CrossRef] [PubMed]
    [Google Scholar]
  13. Sidrim J. J. C. , Maia D. C. , Brilhante R. S. N. , Soares G. D. P. , Cordeiro R. A. , Monteiro A. J. , Rocha M. F. G. . ( 2010; ). Candida species isolated from the gastrointestinal tract of cockatiels (Nymphicus hollandicus): in vitro antifungal susceptibility profile and phospholipase activity. . Vet Microbiol 145:, 324–328. [CrossRef] [PubMed]
    [Google Scholar]
  14. Silva S. , Negri M. , Henriques M. , Oliveira R. , Williams D. W. , Azeredo J. . ( 2012; ). Candida glabrata, Candida parapsilosis and Candida tropicalis: biology, epidemiology, pathogenicity and antifungal resistance. . FEMS Microbiol Rev 36:, 288–305. [CrossRef] [PubMed]
    [Google Scholar]
  15. Spampinato C. , Leonardi D. . ( 2013; ). Candida infections, causes, targets, and resistance mechanisms: traditional and alternative antifungal agents. . BioMed Res Int 2013, 204237.
    [Google Scholar]
  16. Stepanovic S. , Vukovic D. , Dakic I. , Savic B. , Svabic-Vlahovic M. . ( 2000; ). A modified microtiter-plate test for quantification of staphylococcal biofilm formation. . J Microbiol Methods 40:, 175–179. [CrossRef] [PubMed]
    [Google Scholar]
  17. Tavanti A. , Davidson A. D. , Gow N. A. , Maiden M. C. J. , Odds F. C. . ( 2005; ). Candida orthopsilosis and Candida metapsilosis spp. nov. to replace Candida parapsilosis groups II and III. . J Clin Microbiol 43:, 284–292. [CrossRef] [PubMed]
    [Google Scholar]
  18. Tosun I. , Akyuz Z. , Guler N. C. , Gulmez D. , Bayramoglu G. , Kaklikkaya N. , Arikan-Akdagli S. , Aydin F. . ( 2013; ). Distribution, virulence attributes and antifungal susceptibility patterns of Candida parapsilosis complex strains isolated from clinical samples. . Med Mycol 51:, 483–492. [CrossRef] [PubMed]
    [Google Scholar]
  19. Vidotto V. , Pontón J. , Aoki S. , Quindós G. , Mantoan B. , Pugliese A. , Ito-Kuwa S. , Nakamura K. . ( 2004; ). Differences in extracellular enzymatic activity between Candida dubliniensis and Candida albicans isolates. . Rev Iberoam Micol 21:, 70–74.[PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.076216-0
Loading
/content/journal/jmm/10.1099/jmm.0.076216-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error