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Volume 63, Issue 11

Prolonged clonal spreading and dynamic changes in antimicrobial resistance of ST68 among patients who stayed in a respiratory care ward Free

Abstract

From 2007 to 2009, we collected a total of 83 bacteraemic isolates of with reduced susceptibility or resistance to third-generation cephalosporins (TGCs). Isolates were genotyped by PFGE and multilocus sequence typing (MLST). The PFGE patterns revealed two highly correlated clusters (cluster E: nine isolates; cluster G: 22 isolates) associated with this prolonged clonal spreading. Compared with cluster E isolates, cluster G isolates were significantly more likely to harbour (<0.05), and most of these isolates were isolated during a later year than cluster E isolates (<0.05). By MLST analysis, 94 % of cluster E and G isolates (29/31) were ST68. Although no time or space clustering could be identified by the conventional hospital-acquired infection monitoring system, cases caused by cluster E and G isolates were significantly associated with having stayed in our hospital’s respiratory care ward (<0.05). Isolates obtained from patients who had stayed in the respiratory care ward had a significantly higher rate of and positivity, and were more likely to belong to ST68/S68-like (all <0.05). To our knowledge, this is the first report of prolonged clonal spreading caused by ST68 associated with a stay in a long-term care facility. Using epidemiological investigations and PFGE and MLST analyses, we have identified long-term clonal spreading caused by ST68, with extra antimicrobial-resistance genes possibly acquired during the prolonged spreading period.

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© 2014 The Authors
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2014-11-01
2024-04-23
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Prolonged clonal spreading and dynamic changes in antimicrobial resistance of Escherichia coli ST68 among patients who stayed in a respiratory care ward
J. Med. Microbiol. 63, 1531 (2014); https://doi.org/10.1099/jmm.0.075937-0
/content/journal/jmm/10.1099/jmm.0.075937-0
/content/journal/jmm/10.1099/jmm.0.075937-0
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