1887

Abstract

Antibiotic susceptibility testing with the BD Phoenix system on bacterial cell pellets generated from blood culture broths using the Bruker MALDI Sepsityper kit was evaluated. Seventy-six Gram-negative isolates, including 12 with defined multi-resistant phenotypes, had antibiotic susceptibility testing (AST) performed by Phoenix on the cell pellet in parallel with conventional methods. In total, 1414/1444 (97.9 %) of susceptibility tests were concordant, with only 1 (0.07 %) very major error. This novel method has the potential to reduce the turnaround time for AST results by up to a day for Gram-negative bacteraemias.

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2014-12-01
2021-10-17
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References

  1. Barenfanger J., Drake C., Kacich G. 1999; Clinical and financial benefits of rapid bacterial identification and antimicrobial susceptibility testing. J Clin Microbiol 37:1415–1418[PubMed]
    [Google Scholar]
  2. Beekmann S. E., Diekema D. J., Chapin K. C., Doern G. V. 2003; Effects of rapid detection of bloodstream infections on length of hospitalization and hospital charges. J Clin Microbiol 41:3119–3125 [View Article][PubMed]
    [Google Scholar]
  3. Bruker Daltonik. 2012 Bruker Guide to MALDI Sample Preparation – MALDI Preparation Protocols. Revision 2 Bremen: Bruker Daltonik;
    [Google Scholar]
  4. Buchan B. W., Riebe K. M., Ledeboer N. A. 2012; Comparison of the MALDI Biotyper system using Sepsityper specimen processing to routine microbiological methods for identification of bacteria from positive blood culture bottles. J Clin Microbiol 50:346–352 [View Article][PubMed]
    [Google Scholar]
  5. Clark R. B., Lewinski M. A., Loeffelholz M. J., Tibbetts R. J. 2009 In Cumitech 31A, Verification and Validation of Procedures in the Clinical Microbiology Laboratory pp. 13–14 Edited by Sharp S. E. Washington, DC: ASM Press;
    [Google Scholar]
  6. CLSI 2012; Performance Standards for Antimicrobial Susceptibility Testing; 22nd Informational Supplement M100-S22. Wayne, PA: Clinical and Laboratory Standards Institute;
    [Google Scholar]
  7. Ellem J., Partridge S. R., Iredell J. R. 2011; Efficient direct extended-spectrum β-lactamase detection by multiplex real-time PCR: accurate assignment of phenotype by use of a limited set of genetic markers. J Clin Microbiol 49:3074–3077 [View Article][PubMed]
    [Google Scholar]
  8. Espedido B. A., Partridge S. R., Iredell J. R. 2008; bla(IMP-4) in different genetic contexts in Enterobacteriaceae isolates from Australia. Antimicrob Agents Chemother 52:2984–2987 [View Article][PubMed]
    [Google Scholar]
  9. Gaieski D. F., Mikkelsen M. E., Band R. A., Pines J. M., Massone R., Furia F. F., Shofer F. S., Goyal M. 2010; Impact of time to antibiotics on survival in patients with severe sepsis or septic shock in whom early goal-directed therapy was initiated in the emergency department. Crit Care Med 38:1045–1053 [View Article][PubMed]
    [Google Scholar]
  10. Ginn A. N., Zong Z., Wiklendt A. M., Thomas L. C., Merlino J., Gottlieb T., van Hal S., Harkness J., Macleod C.& other authors ( 2013; Limited diversity in the gene pool allows prediction of third-generation cephalosporin and aminoglycoside resistance in Escherichia coli and Klebsiella pneumoniae. Int J Antimicrob Agents 42:19–26 [View Article][PubMed]
    [Google Scholar]
  11. Gray T. J., Thomas L., Olma T., Iredell J. R., Chen S. C. 2013; Rapid identification of Gram-negative organisms from blood culture bottles using a modified extraction method and MALDI-TOF mass spectrometry. Diagn Microbiol Infect Dis 77:110–112 [View Article][PubMed]
    [Google Scholar]
  12. Klein S., Zimmermann S., Köhler C., Mischnik A., Alle W., Bode K. A. 2012; Integration of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry in blood culture diagnostics: a fast and effective approach. J Med Microbiol 61:323–331 [View Article][PubMed]
    [Google Scholar]
  13. Kok J., Thomas L. C., Olma T., Chen S. C. A., Iredell J. R. 2011; Identification of bacteria in blood culture broths using matrix-assisted laser desorption-ionization Sepsityper™ and time of flight mass spectrometry. PLoS ONE 6:e23285 [View Article][PubMed]
    [Google Scholar]
  14. Kumar A., Roberts D., Wood K. E., Light B., Parrillo J. E., Sharma S., Suppes R., Feinstein D., Zanotti S.& other authors ( 2006; Duration of hypotension before initiation of effective antimicrobial therapy is the critical determinant of survival in human septic shock. Crit Care Med 34:1589–1596 [View Article][PubMed]
    [Google Scholar]
  15. Partridge S. R., Ellem J. A., Tetu S. G., Zong Z., Paulsen I. T., Iredell J. R. 2011; Complete sequence of pJIE143, a pir-type plasmid carrying ISEcp1-blaCTX-M-15 from an Escherichia coli ST131 isolate. Antimicrob Agents Chemother 55:5933–5935 [View Article][PubMed]
    [Google Scholar]
  16. Partridge S. R., Ginn A. N., Paulsen I. T., Iredell J. R. 2012; pEl1573 carrying blaIMP-4, from Sydney, Australia, is closely related to other IncL/M plasmids. Antimicrob Agents Chemother 56:6029–6032 [View Article][PubMed]
    [Google Scholar]
  17. Shoma S., Kamruzzaman M., Ginn A. N., Iredell J. R., Partridge S. R. 2014; Characterization of multidrug-resistant Klebsiella pneumoniae from Australia carrying blaNDM-1.. Diagn Microbiol Infect Dis 78:93–97 [View Article][PubMed]
    [Google Scholar]
  18. Shorr A. F., Micek S. T., Jackson W. L. Jr, Kollef M. H. 2007; Economic implications of an evidence-based sepsis protocol: can we improve outcomes and lower costs?. Crit Care Med 35:1257–1262 [View Article][PubMed]
    [Google Scholar]
  19. Stevenson L. G., Drake S. K., Murray P. R. 2010; Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 48:444–447 [View Article][PubMed]
    [Google Scholar]
  20. Valenzuela J. K., Thomas L., Partridge S. R., van der Reijden T., Dijkshoorn L., Iredell J. 2007; Horizontal gene transfer in a polyclonal outbreak of carbapenem-resistant Acinetobacter baumannii. J Clin Microbiol 45:453–460 [View Article][PubMed]
    [Google Scholar]
  21. Wimmer J. L., Long S. W., Cernoch P., Land G. A., Davis J. R., Musser J. M., Olsen R. J. 2012; Strategy for rapid identification and antibiotic susceptibility testing of Gram-negative bacteria directly recovered from positive blood cultures using the Bruker MALDI Biotyper and the BD Phoenix system. J Clin Microbiol 50:2452–2454 [View Article][PubMed]
    [Google Scholar]
  22. Yan Y., He Y., Maier T., Quinn C., Shi G., Li H., Stratton C. W., Kostrzewa M., Tang Y. W. 2011; Improved identification of yeast species directly from positive blood culture media by combining Sepsityper specimen processing and Microflex analysis with the matrix-assisted laser desorption ionization Biotyper system. J Clin Microbiol 49:2528–2532 [View Article][PubMed]
    [Google Scholar]
  23. Yonetani S., Okazaki M., Araki K., Makino H., Fukugawa Y., Okuyama T., Ohnishi H., Watanabe T. 2012; Direct inoculation method using BacT/ALERT 3D and BD Phoenix System allows rapid and accurate identification and susceptibility testing for both Gram-positive cocci and Gram-negative rods in aerobic blood cultures. Diagn Microbiol Infect Dis 73:129–134 [View Article][PubMed]
    [Google Scholar]
  24. Zong Z., Partridge S. R., Iredell J. R. 2008a; RmtC 16S rRNA methyltransferase in Australia. Antimicrob Agents Chemother 52:794–795 [View Article][PubMed]
    [Google Scholar]
  25. Zong Z., Partridge S. R., Thomas L., Iredell J. R. 2008b; Dominance of blaCTX-M within an Australian extended-spectrum β-lactamase gene pool. Antimicrob Agents Chemother 52:4198–4202 [View Article][PubMed]
    [Google Scholar]
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