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Volume 63, Issue 10

recurrence is characterized by pro-inflammatory peripheral blood mononuclear cell (PBMC) phenotype Free

Abstract

infection (CDI) is a prevalent nosocomial and increasingly community-acquired problem. Little is known about the productive cellular response in patients. We used flow cytometry to define inflammatory (Th1 and Th17) and regulatory [Foxp3 T-regulatory (Treg)] cells present in circulating peripheral blood mononuclear cells (PBMC) from CDI patients. We consented 67 inpatients that tested either positive or negative for CDI and 16 healthy controls and compared their PBMC phenotypes. PBMC were collected, isolated, and stained for CD3, CD8 and either IL17 (Th17), IFN-γ (Th1) or Foxp3 (Treg) and analysed using flow cytometry. Twenty thousand events were collected in the lymphocyte gate (gate 1) and T-cell phenotypes were defined. CDI patients who clear the primary initial infection have greater numbers of non-CD3 PBMC. CDI patients who develop recurrence of CDI have a greater percentage of CD3CD8, CD3CD4Foxp3 and fewer low granular CD3Foxp3 PBMC. These patients have greater numbers of IFN-γ-producing lymphocytes, as well as PBMC phenotypes represented by increased IFN-γ- and IL17-co-expressing CD4CD3. This initial pro-inflammatory phenotype decreases with repeated recurrence, demonstrating importance of timing of sample collection and history of symptoms. Patients with a history of recurrence had increased Foxp3CD3CD4 and IL17CD3CD4 populations. Hence, CDI recurrence is hallmarked by greater numbers of circulating CD3 lymphocytes skewed towards a Th1/Th17 inflammatory population as well as possible immune plasticity (Th17/Treg).

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© 2014 The Authors
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2014-10-01
2024-04-23
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Clostridium difficile recurrence is characterized by pro-inflammatory peripheral blood mononuclear cell (PBMC) phenotype
J. Med. Microbiol. 63, 1260 (2014); https://doi.org/10.1099/jmm.0.075382-0
/content/journal/jmm/10.1099/jmm.0.075382-0
/content/journal/jmm/10.1099/jmm.0.075382-0
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