1887

Abstract

Forty-five lactic acid bacteria (LAB) were isolated from the vaginal specimens of healthy fertile women, and the identities of the bacteria were confirmed by sequencing of their 16S rDNA genes. Among these bacteria, only four isolates were able to resist and survive in low pH, bile salts and simulated digestion conditions. 2HL, 6HL, 36YL and 5BL showed the best resistance to these conditions. These strains were evaluated further to assess their ability to adhere to human intestinal Caco-2 cells. 2HL and 6HL were the most adherent strains. tests under neutralized pH proved the antimicrobial activity of both strains. Results revealed that the growth of O26, and was suppressed by both LAB strains. The antibiotic susceptibility tests showed that these strains were sensitive to all nine antibiotics: vancomycin, tetracycline, ampicillin, penicillin, gentamicin, erythromycin, clindamycin, sulfamethoxazole and chloramphenicol. These data suggest that 6HL and 2HL could be examined further for their useful properties and could be developed as new probiotics.

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2014-08-01
2019-12-14
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References

  1. Altschul S. F. , Madden T. L. , Schäffer A. A. , Zhang J. , Zhang Z. , Miller W. , Lipman D. J. . ( 1997; ). Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. . Nucleic Acids Res 25:, 3389–3402. [CrossRef] [PubMed]
    [Google Scholar]
  2. Andersson H. , Asp N. G. , Bruce A. , Roos S. , Wadstrom T. , Wold A. E. . ( 2001; ). Health effects of probiotics and prebiotics. A literature review on human studies. . Scand J Nutr 45:, 58–75.
    [Google Scholar]
  3. Aslim B. , Kilic E. . ( 2006; ). Some probiotic properties of vaginal lactobacilli isolated from healthy women. . Jpn J Infect Dis 59:, 249–253.[PubMed]
    [Google Scholar]
  4. Barouei J. , Adams M. C. , Hodgson D. M. . ( 2009; ). Prophylactic role of maternal administration of probiotics in the prevention of irritable bowel syndrome. . Med Hypotheses 73:, 764–767. [CrossRef] [PubMed]
    [Google Scholar]
  5. Barzegari A. , Saei A. A. . ( 2012; ). Designing probiotics with respect to the native microbiome. . Future Microbiol 7:, 571–575.[CrossRef]
    [Google Scholar]
  6. Bezkorovainy A. . ( 2001; ). Probiotics: determinants of survival and growth in the gut. . Am J Clin Nutr 73: (Suppl), 399S–405S.[PubMed]
    [Google Scholar]
  7. Binns C. , Lee M. K. . ( 2010; ). The use of probiotics to prevent diarrhea in young children attending child care centers: a review. . Journal of Experimental & Clinical Medicine 2:, 269–273. [CrossRef]
    [Google Scholar]
  8. Bunselmeyer B. , Buddendick K. . ( 2010; ). Probiotics and prebiotics—prevention and therapy in atopic eczema. . In Bioactive Foods in Promoting Health, pp. 279–292. Edited by Watson R. R. , Preedy V. R. . . Boston:: Academic Press;. [CrossRef]
    [Google Scholar]
  9. Burton J. P. , Cadieux P. A. , Reid G. . ( 2003; ). Improved understanding of the bacterial vaginal microbiota of women before and after probiotic instillation. . Appl Environ Microbiol 69:, 97–101.[CrossRef]
    [Google Scholar]
  10. Damaskos D. , Kolios G. . ( 2008; ). Probiotics and prebiotics in inflammatory bowel disease: microflora ‘on the scope’. . Br J Clin Pharmacol 65:, 453–467. [CrossRef] [PubMed]
    [Google Scholar]
  11. Dimitonova S. P. , Danova S. T. , Serkedjieva J. P. , Bakalov B. V. . ( 2007; ). Antimicrobial activity and protective properties of vaginal lactobacilli from healthy Bulgarian women. . Anaerobe 13:, 178–184. [CrossRef] [PubMed]
    [Google Scholar]
  12. Donders G. G. , Van Calsteren K. , Bellen G. , Reybrouck R. , Van den Bosch T. , Riphagen I. , Van Lierde S. . ( 2009; ). Predictive value for preterm birth of abnormal vaginal flora, bacterial vaginosis and aerobic vaginitis during the first trimester of pregnancy. . BJOG 116:, 1315–1324. [CrossRef] [PubMed]
    [Google Scholar]
  13. Dubernet S. , Desmasures N. , Guéguen M. . ( 2002; ). A PCR-based method for identification of lactobacilli at the genus level. . FEMS Microbiol Lett 214:, 271–275. [CrossRef] [PubMed]
    [Google Scholar]
  14. Dunne C. , O’Mahony L. , Murphy L. , Thornton G. , Morrissey D. , O’Halloran S. , Feeney M. , Flynn S. , Fitzgerald G. et al. ( 2001; ). In vitro selection criteria for probiotic bacteria of human origin: correlation with in vivo findings. . Am J Clin Nutr 73: (Suppl), 386S–392S.[PubMed]
    [Google Scholar]
  15. Friedman G. . ( 2012; ). The role of probiotics in the prevention and treatment of antibiotic-associated diarrhea and Clostridium difficile colitis. . Gastroenterol Clin North Am 41:, 763–779. [CrossRef] [PubMed]
    [Google Scholar]
  16. Geier M. S. , Butler R. N. , Howarth G. S. . ( 2007; ). Inflammatory bowel disease: current insights into pathogenesis and new therapeutic options; probiotics, prebiotics and synbiotics. . Int J Food Microbiol 115:, 1–11. [CrossRef] [PubMed]
    [Google Scholar]
  17. Gionchetti P. , Amadini C. , Rizzello F. , Venturi A. , Palmonari V. , Morselli C. , Romagnoli R. , Campieri M. . ( 2002; ). Probiotics – role in inflammatory bowel disease. . Dig Liver Dis 34: (Suppl 2), S58–S62. [CrossRef] [PubMed]
    [Google Scholar]
  18. Gorbach S. L. . ( 2002; ). Probiotics in the third millennium. . Dig Liver Dis 34: (Suppl 2), S2–S7. [CrossRef] [PubMed]
    [Google Scholar]
  19. Hoesl C. E. , Altwein J. E. . ( 2005; ). The probiotic approach: an alternative treatment option in urology. . Eur Urol 47:, 288–296. [CrossRef] [PubMed]
    [Google Scholar]
  20. Hoveyda N. , Heneghan C. , Mahtani K. R. , Perera R. , Roberts N. , Glasziou P. . ( 2009; ). A systematic review and meta-analysis: probiotics in the treatment of irritable bowel syndrome. . BMC Gastroenterol 9:, 15–23. [CrossRef] [PubMed]
    [Google Scholar]
  21. Huang Y. , Zheng Y. . ( 2010; ). The probiotic Lactobacillus acidophilus reduces cholesterol absorption through the down-regulation of Niemann–Pick C1-like 1 in Caco-2 cells. . Br J Nutr 103:, 473–478. [CrossRef] [PubMed]
    [Google Scholar]
  22. Johnson A. P. , Uttley A. H. C. , Woodford N. , George R. C. . ( 1990; ). Resistance to vancomycin and teicoplanin: an emerging clinical problem. . Clin Microbiol Rev 3:, 280–291.[PubMed]
    [Google Scholar]
  23. Klaenhammer T. R. , Kullen M. J. . ( 1999; ). Selection and design of probiotics. . Int J Food Microbiol 50:, 45–57. [CrossRef] [PubMed]
    [Google Scholar]
  24. Kuitunen M. , Kukkonen A. K. , Savilahti E. . ( 2012; ). Impact of maternal allergy and use of probiotics during pregnancy on breast milk cytokines and food antibodies and development of allergy in children until 5 years. . Int Arch Allergy Immunol 159:, 162–170. [CrossRef] [PubMed]
    [Google Scholar]
  25. Lilly D. M. , Stillwell R. H. . ( 1965; ). Probiotics: growth-promoting factors produced by microorganisms. . Science 147:, 747–748. [CrossRef] [PubMed]
    [Google Scholar]
  26. Lim K. S. , Huh C. S. , Baek Y. J. . ( 1993; ). Antimicrobial susceptibility of bifidobacteria. . J Dairy Sci 76:, 2168–2174. [CrossRef] [PubMed]
    [Google Scholar]
  27. Liong M. T. . ( 2008; ). Roles of probiotics and prebiotics in colon cancer prevention: postulated mechanisms and in vivo evidence. . Int J Mol Sci 9:, 854–863. [CrossRef] [PubMed]
    [Google Scholar]
  28. MacPhee R. A. , Hummelen R. , Bisanz J. E. , Miller W. L. , Reid G. . ( 2010; ). Probiotic strategies for the treatment and prevention of bacterial vaginosis. . Expert Opin Pharmacother 11:, 2985–2995. [CrossRef] [PubMed]
    [Google Scholar]
  29. Maldonado N. C. , de Ruiz C. S. , Otero M. C. , Sesma F. , Nader-Macías M. E. . ( 2012; ). Lactic acid bacteria isolated from young calves – characterization and potential as probiotics. . Res Vet Sci 92:, 342–349. [CrossRef] [PubMed]
    [Google Scholar]
  30. Metchnikoff E. . ( 1907; ). The Prolongation of Life. London:: William Heinemann;.
    [Google Scholar]
  31. Mirzaei H. , Barzgari A. . ( 2012; ). Isolation and Molecular Study of Potentially Probiotic Lactobacilli in Traditional White Cheese of Tabriz in Iran. . Annals of Biological Research 3:, 2213–2216.
    [Google Scholar]
  32. Nam H. , Whang K. , Lee Y. . ( 2007; ). Analysis of vaginal lactic acid-producing bacteria in healthy women. . J Microbiol 45:, 515–520.[PubMed]
    [Google Scholar]
  33. Nowroozi J. , Mirzaii M. , Norouzi M. . ( 2004; ). Study of Lactobacillus as probiotic bacteria. . Iran J Public Health 33 (2):, 1–7.
    [Google Scholar]
  34. Nueno-Palop C. , Narbad A. . ( 2011; ). Probiotic assessment of Enterococcus faecalis CP58 isolated from human gut. . Int J Food Microbiol 145:, 390–394. [CrossRef] [PubMed]
    [Google Scholar]
  35. Okkers D. J. , Dicks L. M. T. , Silvester M. , Joubert J. J. , Odendaal H. J. . ( 1999; ). Characterization of pentocin TV35b, a bacteriocin-like peptide isolated from Lactobacillus pentosus with a fungistatic effect on Candida albicans . . J Appl Microbiol 87:, 726–734. [CrossRef] [PubMed]
    [Google Scholar]
  36. Ouwehand A. C. , Kirjavainen P. V. , Shortt C. , Salminen S. . ( 1999; ). Probiotics: mechanisms and established effects. . Int Dairy J 9:, 43–52. [CrossRef]
    [Google Scholar]
  37. Ouwehand A. C. , Vesterlund S. . ( 2004; ). Antimicrobial Components from Lactic Acid Bacteria. Lactic Acid Bacteria Microbiological and Functional Aspects. New York:: Marcel Dekker Inc;.
    [Google Scholar]
  38. Parkes G. C. . ( 2010; ). The role of probiotics in the treatment of irritable bowel syndrome. . In Bioactive Foods in Promoting Health, pp. 513–528. Edited by Ronald Ross W. , Victor R. P. . . Boston:: Academic Press;. [CrossRef]
    [Google Scholar]
  39. Pereira D. I. A. , Gibson G. R. . ( 2002; ). Cholesterol assimilation by lactic acid bacteria and bifidobacteria isolated from the human gut. . Appl Environ Microbiol 68:, 4689–4693. [CrossRef] [PubMed]
    [Google Scholar]
  40. Petricevic L. , Domig K. J. , Nierscher F. J. , Krondorfer I. , Janitschek C. , Kneifel W. , Kiss H. . ( 2012; ). Characterisation of the oral, vaginal and rectal Lactobacillus flora in healthy pregnant and postmenopausal women. . Eur J Obstet Gynecol Reprod Biol 160:, 93–99. [CrossRef] [PubMed]
    [Google Scholar]
  41. Rahmati M. , Bannazadeh H. , Fazli D. , Tahmasebzadeh F. , Farrokhi A. , Rasmi Y. . ( 2011; ). The apoptotic and cytotoxic effects of Polygonum avicular extract on Hela-S cervical cancer cell line. . African Journal of Biochemistry Research 5:, 373–378.
    [Google Scholar]
  42. Redondo-Lopez V. , Cook R. L. , Sobel J. D. . ( 1990; ). Emerging role of lactobacilli in the control and maintenance of the vaginal bacterial microflora. . Rev Infect Dis 12:, 856–872. [CrossRef] [PubMed]
    [Google Scholar]
  43. Reid G. , Bocking A. . ( 2003; ). The potential for probiotics to prevent bacterial vaginosis and preterm labor. . Am J Obstet Gynecol 189:, 1202–1208. [CrossRef] [PubMed]
    [Google Scholar]
  44. Reid G. , Dols J. , Miller W. . ( 2009; ). Targeting the vaginal microbiota with probiotics as a means to counteract infections. . Curr Opin Clin Nutr Metab Care 12:, 583–587. [CrossRef] [PubMed]
    [Google Scholar]
  45. Resta-Lenert S. C. , Barrett K. E. . ( 2009; ). Modulation of intestinal barrier properties by probiotics: role in reversing colitis. . Ann N Y Acad Sci 1165:, 175–182. [CrossRef] [PubMed]
    [Google Scholar]
  46. Saarela M. , Mogensen G. , Fondén R. , Mättö J. , Mattila-Sandholm T. . ( 2000; ). Probiotic bacteria: safety, functional and technological properties. . J Biotechnol 84:, 197–215. [CrossRef] [PubMed]
    [Google Scholar]
  47. Salminen S. , von Wright A. , Morelli L. , Marteau P. , Brassart D. , de Vos W. M. , Fondén R. , Saxelin M. , Collins K. et al. ( 1998; ). Demonstration of safety of probiotics – a review. . Int J Food Microbiol 44:, 93–106. [CrossRef] [PubMed]
    [Google Scholar]
  48. Schillinger U. , Lücke F. K. . ( 1987; ). Identification of lactobacilli from meat and meat products. . Food Microbiol 4:, 199–208. [CrossRef]
    [Google Scholar]
  49. Seiquer I. , Aspe T. , Vaquero P. , Navarro P. . ( 2001; ). Effects of heat treatment of casein in the presence of reducing sugars on calcium bioavailability: in vitro and in vivo assays. . J Agric Food Chem 49:, 1049–1055. [CrossRef] [PubMed]
    [Google Scholar]
  50. Serban D. E. . ( 2014; ). Gastrointestinal cancers: influence of gut microbiota, probiotics and prebiotics. . Cancer Lett 345:, 258–270. [CrossRef] [PubMed]
    [Google Scholar]
  51. Shi Y. , Chen L. , Tong J. , Xu C. . ( 2009; ). Preliminary characterization of vaginal microbiota in healthy Chinese women using cultivation-independent methods. . J Obstet Gynaecol Res 35:, 525–532. [CrossRef] [PubMed]
    [Google Scholar]
  52. Strus M. , Chmielarczyk A. , Kochan P. , Adamski P. , Chełmicki Z. , Chełmicki A. , Pałucha A. , Heczko P. B. . ( 2012; ). Studies on the effects of probiotic Lactobacillus mixture given orally on vaginal and rectal colonization and on parameters of vaginal health in women with intermediate vaginal flora. . Eur J Obstet Gynecol Reprod Biol 163:, 210–215. [CrossRef] [PubMed]
    [Google Scholar]
  53. Surawicz C. M. . ( 2003; ). Probiotics, antibiotic-associated diarrhoea and Clostridium difficile diarrhoea in humans. . Best Pract Res Clin Gastroenterol 17:, 775–783. [CrossRef] [PubMed]
    [Google Scholar]
  54. Tomás M. S. , Bru E. , Nader-Macías M. E. . ( 2003; ). Comparison of the growth and hydrogen peroxide production by vaginal probiotic lactobacilli under different culture conditions. . Am J Obstet Gynecol 188:, 35–44. [CrossRef] [PubMed]
    [Google Scholar]
  55. Tuomola E. , Crittenden R. , Playne M. , Isolauri E. , Salminen S. . ( 2001; ). Quality assurance criteria for probiotic bacteria. . Am J Clin Nutr 73: (Suppl), 393S–398S.[PubMed]
    [Google Scholar]
  56. Tynkkynen S. , Singh K. V. , Varmanen P. . ( 1998; ). Vancomycin resistance factor of Lactobacillus rhamnosus GG in relation to enterococcal vancomycin resistance (van) genes. . Int J Food Microbiol 41:, 195–204. [CrossRef] [PubMed]
    [Google Scholar]
  57. Vernazza C. L. , Gibson G. R. , Rastall R. A. . ( 2006; ). Carbohydrate preference, acid tolerance and bile tolerance in five strains of Bifidobacterium . . J Appl Microbiol 100:, 846–853. [CrossRef] [PubMed]
    [Google Scholar]
  58. Wickens K. , Black P. N. , Stanley T. V. , Mitchell E. , Fitzharris P. , Tannock G. W. , Purdie G. , Crane J. . Probiotic Study Group ( 2008; ). A differential effect of 2 probiotics in the prevention of eczema and atopy: a double-blind, randomized, placebo-controlled trial. . J Allergy Clin Immunol 122:, 788–794. [CrossRef] [PubMed]
    [Google Scholar]
  59. Woodford N. , Johnson A. P. , Morrison D. , Speller D. C. E. . ( 1995; ). Current perspectives on glycopeptide resistance. . Clin Microbiol Rev 8:, 585–615.[PubMed]
    [Google Scholar]
  60. Young R. J. , Huffman S. . ( 2003; ). Probiotic use in children. . J Pediatr Health Care 17:, 277–283. [CrossRef] [PubMed]
    [Google Scholar]
  61. Zavisic G. , Radulovic Z. , Vranic V. , Begovic J. , Topisirovic L. , Strahinic I. . ( 2011; ). Characterization and antimicrobial activity of vaginal Lactobacillus isolate. . Archives of Biological Science Belgrade 63:, 29–35. [CrossRef]
    [Google Scholar]
  62. Zhu Y. , Michelle Luo T. , Jobin C. , Young H. A. . ( 2011; ). Gut microbiota and probiotics in colon tumorigenesis. . Cancer Lett 309:, 119–127. [CrossRef] [PubMed]
    [Google Scholar]
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