Characterization of the blaKPC-2 and blaKPC-3 genes and the novel blaKPC-15 gene in Klebsiella pneumoniae

Dongguo Wang; Wei Hou; Jiayu Chen; Yonghua Mou; Linjun Yang; Liqin Yang; Xiulian Sun; Meiyun Chen

doi:10.1099/jmm.0.073841-0

Volume 63, Issue 7

Research Article

Free

Characterization of the bla KPC-2 and bla KPC-3 genes and the novel bla KPC-15 gene in Klebsiella pneumoniae

Dongguo Wang^1,†, Wei Hou^2,†, Jiayu Chen^3,†, Yonghua Mou^4,†, Linjun Yang^5,†, Liqin Yang⁶, Xiulian Sun⁷ and Meiyun Chen⁸
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Affiliations: ¹ Department of Clinical Laboratory Medicine, Taizhou Municipal Hospital and Institute of Molecular Diagnostics, Taizhou University, Taizhou, Zhejiang, PR China ² Department of Infectious Diseases, Taizhou Municipal Hospital, Taizhou University, Taizhou, Zhejiang, PR China ³ Department of Laboratory Medicine, Medical School of Taizhou University and Institute of Molecular Diagnostics, Taizhou University, Taizhou, Zhejiang, PR China ⁴ Department of Hepatobiliary Surgery, Taizhou Municipal Hospital, Taizhou University, Taizhou, Zhejiang, PR China ⁵ Department of Thyroid and Breast Surgery, Taizhou Municipal Hospital, Taizhou University, Taizhou, Zhejiang, PR China ⁶ Department of Orthopaedics, Taizhou Municipal Hospital, Taizhou University, Taizhou, Zhejiang, PR China ⁷ Emergency Department, Taizhou Municipal Hospital, Taizhou University, Taizhou, Zhejiang, PR China ⁸ Department of Paediatrics, Taizhou Municipal Hospital, Taizhou University, Taizhou, Zhejiang, PR China
Correspondence Dongguo Wang [email protected] Jiayu Chen [email protected]

† These authors contributed equally to the manuscript.
Published: 01 July 2014 https://doi.org/10.1099/jmm.0.073841-0

Abstract

Three Klebsiella pneumoniae isolates exhibiting high-level resistance to carbapenem were analysed by PCR, PFGE, gene mapping, plasmid conjugation and Southern blot hybridization using a bla KPC probe. In addition to the frequently reported bla KPC-2 and bla KPC-3 genes, a novel bla KPC-15 gene was identified in one of the isolates. The results of plasmid analysis and Southern blot hybridization revealed that the three bla KPC genes were located on transferable plasmids exhibiting three different patterns. The patterns A, B and C were observed in the genetic makeup of each individual plasmid, and all three structures contained ISKpn6-like and ISKpn8 transposons. The results of the gene mapping and hybridization experiments performed with the bla KPC probe demonstrated that the plasmids harboured the three genes at approximately the 85.0, 54.0 and 73.0 kb positions. The study concluded that carbapenem resistance in the three isolates was primarily due to the production of carbapenem-hydrolysing β-lactamase.

Received: 28/01/2014
Accepted: 07/04/2014
Published Online: 01/07/2014

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Characterization of the blaKPC-2 and blaKPC-3 genes and the novel blaKPC-15 gene in Klebsiella pneumoniae

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Volume 63, Issue 7

Research Article

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Characterization of the bla KPC-2 and bla KPC-3 genes and the novel bla KPC-15 gene in Klebsiella pneumoniae

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