1887

Abstract

Three isolates exhibiting high-level resistance to carbapenem were analysed by PCR, PFGE, gene mapping, plasmid conjugation and Southern blot hybridization using a probe. In addition to the frequently reported and genes, a novel gene was identified in one of the isolates. The results of plasmid analysis and Southern blot hybridization revealed that the three genes were located on transferable plasmids exhibiting three different patterns. The patterns A, B and C were observed in the genetic makeup of each individual plasmid, and all three structures contained IS-like and IS transposons. The results of the gene mapping and hybridization experiments performed with the probe demonstrated that the plasmids harboured the three genes at approximately the 85.0, 54.0 and 73.0 kb positions. The study concluded that carbapenem resistance in the three isolates was primarily due to the production of carbapenem-hydrolysing β-lactamase.

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2014-07-01
2019-12-08
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