1887

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Volume 63, Issue 12

Cross-protective effect of a novel multi-antigen-chimeric vaccine against and infection in mice Free

Abstract

Staphylococcal and streptococcal species are the most common pathogens that cause bovine mastitis. Induction of a broad-spectrum protective immunity against staphylococci and streptococci by combining multiple antigens into a single vaccine is highlighted. To develop a universal vaccine candidate, a GapC-tIsdB-TRAP (GIT) construct was generated. The GIT contained the truncated GapC from , and truncated IsdB and full-length TRAP from . The humoral and cellular immune responses elicited by GIT were evaluated in mice. Antibody levels against GIT displayed a consistent tendency with antibody levels against GapC, IsdB and TRAP. The level of IFN-γ was higher in the GIT group than in the IsdB group (<0.05), and the level of IL-4 was higher in the GIT group than in the GapC or TRAP groups (<0.05). The GIT group showed an improved protection against in comparison with GapC group. A significant difference in challenge test was detected between the GIT group and the IsdB or TRAP groups (<0.05) in per cent survival of mice, and a synergistic immunoprotection against or was produced in the GIT group. These results suggested that the GIT would be a promising common vaccine candidate against and .

  • Received:
  • Accepted:
  • Published Online:
© 2014 The Authors
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2014-12-01
2024-04-25
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Cross-protective effect of a novel multi-antigen-chimeric vaccine against Streptococcus and Staphylococcus aureus infection in mice
J. Med. Microbiol. 63, 1732 (2014); https://doi.org/10.1099/jmm.0.073593-0
/content/journal/jmm/10.1099/jmm.0.073593-0
/content/journal/jmm/10.1099/jmm.0.073593-0
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