1887

Abstract

Staphylococcal and streptococcal species are the most common pathogens that cause bovine mastitis. Induction of a broad-spectrum protective immunity against staphylococci and streptococci by combining multiple antigens into a single vaccine is highlighted. To develop a universal vaccine candidate, a GapC-tIsdB-TRAP (GIT) construct was generated. The GIT contained the truncated GapC from , and truncated IsdB and full-length TRAP from . The humoral and cellular immune responses elicited by GIT were evaluated in mice. Antibody levels against GIT displayed a consistent tendency with antibody levels against GapC, IsdB and TRAP. The level of IFN-γ was higher in the GIT group than in the IsdB group (<0.05), and the level of IL-4 was higher in the GIT group than in the GapC or TRAP groups (<0.05). The GIT group showed an improved protection against in comparison with GapC group. A significant difference in challenge test was detected between the GIT group and the IsdB or TRAP groups (<0.05) in per cent survival of mice, and a synergistic immunoprotection against or was produced in the GIT group. These results suggested that the GIT would be a promising common vaccine candidate against and .

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.073593-0
2014-12-01
2021-01-25
Loading full text...

Full text loading...

/deliver/fulltext/jmm/63/12/1732.html?itemId=/content/journal/jmm/10.1099/jmm.0.073593-0&mimeType=html&fmt=ahah

References

  1. Bagnoli F., Bertholet S., Grandi G. 2012; Inferring reasons for the failure of Staphylococcus aureus vaccines in clinical trials. Front Cell Infect Microbiol 2:1–4 [CrossRef][PubMed]
    [Google Scholar]
  2. Bessoles S., Fouret F., Dudal S., Besra G. S., Sanchez F., Lafont V. 2008; IL-2 triggers specific signaling pathways in human NKT cells leading to the production of pro- and anti-inflammatory cytokines. J Leukoc Biol 84:224–233 [CrossRef][PubMed]
    [Google Scholar]
  3. Bolton A., Song X. M., Willson P., Fontaine M. C., Potter A. A., Perez-Casal J. 2004; Use of the surface proteins GapC and Mig of Streptococcus dysgalactiae as potential protective antigens against bovine mastitis. Can J Microbiol 50:423–432 [CrossRef][PubMed]
    [Google Scholar]
  4. Boskabady M. H., Mehrjardi S. S., Rezaee A., Rafatpanah H., Jalali S. 2013; The impact of Zataria multiflora Boiss extract on in vitro and in vivo Th1/Th2 cytokine (IFN-γ/IL4) balance. J Ethnopharmacol 150:1024–1031 [CrossRef][PubMed]
    [Google Scholar]
  5. Bowdish D. M., Davidson D. J., Scott M. G., Hancock R. E. 2005; Immunomodulatory activities of small host defense peptides. Antimicrob Agents Chemother 49:1727–1732 [CrossRef][PubMed]
    [Google Scholar]
  6. DeDent A., Kim H. K., Missiakas D., Schneewind O. 2012; Exploring Staphylococcus aureus pathways to disease for vaccine development. Semin Immunopathol 34:317–333 [CrossRef][PubMed]
    [Google Scholar]
  7. Fontaine M. C., Perez-Casal J., Song X. M., Shelford J., Willson P. J., Potter A. A. 2002; Immunisation of dairy cattle with recombinant Streptococcus uberis GapC or a chimeric CAMP antigen confers protection against heterologous bacterial challenge. Vaccine 20:2278–2286 [CrossRef][PubMed]
    [Google Scholar]
  8. Fu Q., Wei Z. G., Liu X. H., Xiao P. P., Lu Z. H., Chen Y. S. 2013; Glyceraldehyde-3-phosphate dehydrogenase, an immunogenic Streptococcus equi ssp. zooepidemicus adhesion protein and protective antigen. J Microbiol Biotechnol 23:579–585 [CrossRef][PubMed]
    [Google Scholar]
  9. Gil M. L., Dagan S., Eren R., Gozalbo D. 2006; Evaluation of the usefulness of anti-glyceraldehyde-3-phosphate dehydrogenase antibodies as a treatment for invasive candidiasis in a murine model. Antonie van Leeuwenhoek 89:345–350 [CrossRef][PubMed]
    [Google Scholar]
  10. Goji N., Potter A. A., Perez-Casal J. 2004; Characterization of two proteins of Staphylococcus aureus isolated from bovine clinical mastitis with homology to glyceraldehyde-3-phosphate dehydrogenase. Vet Microbiol 99:269–279 [CrossRef][PubMed]
    [Google Scholar]
  11. Hoelzle K., Doser S., Ritzmann M., Heinritzi K., Palzer A., Elicker S., Kramer M., Felder K. M., Hoelzle L. E. 2009; Vaccination with the Mycoplasma suis recombinant adhesion protein MSG1 elicits a strong immune response but fails to induce protection in pigs. Vaccine 27:5376–5382 [CrossRef][PubMed]
    [Google Scholar]
  12. Joshi A., Pancari G., Cope L., Bowman E. P., Cua D., Proctor R. A., McNeely T. 2012; Immunization with Staphylococcus aureus iron regulated surface determinant B (IsdB) confers protection via Th17/IL17 pathway in a murine sepsis model. Hum Vaccin Immunother 8:336–346 [CrossRef][PubMed]
    [Google Scholar]
  13. Kerro-Dego O., Prysliak T., Perez-Casal J., Potter A. A. 2012; Role of GapC in the pathogenesis of Staphylococcus aureus.. Vet Microbiol 156:443–447 [CrossRef][PubMed]
    [Google Scholar]
  14. Krieg A. M. 2000; Immune effects and mechanisms of action of CpG motifs. Vaccine 19:618–622 [CrossRef][PubMed]
    [Google Scholar]
  15. Leigh J. A. 2002; Immunisation of dairy cattle with recombinant Streptococcus uberis GapC or a chimeric CAMP antigen confers protection against heterologous bacterial challenge. M.C. Fontaine et al. [Vaccine 20 (2002) 2278-2286]. Vaccine 20:3047–3048 [CrossRef][PubMed]
    [Google Scholar]
  16. Leitner G., Krifucks O., Kiran M. D., Balaban N. 2011; Vaccine development for the prevention of staphylococcal mastitis in dairy cows. Vet Immunol Immunopathol 142:25–35 [CrossRef][PubMed]
    [Google Scholar]
  17. Li X., Wu H., Zhang M., Liang S., Xiao J., Wang Q., Liu Q., Zhang Y. 2012; Secreted glyceraldehyde-3-phosphate dehydrogenase as a broad spectrum vaccine candidate against microbial infection in aquaculture. Lett Appl Microbiol 54:1–9 [CrossRef][PubMed]
    [Google Scholar]
  18. Liu Y., Oshima S., Kawai K. 2007; Glyceraldehyde-3-phosphate dehydrogenase of Edwardsiella tarda has protective antigenicity against Vibrio anguillarum in Japanese flounder. Dis Aquat Organ 75:217–220 [CrossRef][PubMed]
    [Google Scholar]
  19. Madureira P., Andrade E. B., Gama B., Oliveira L., Moreira S., Ribeiro A., Correia-Neves M., Trieu-Cuot P., Vilanova M., Ferreira P. 2011; Inhibition of IL-10 production by maternal antibodies against Group B Streptococcus GAPDH confers immunity to offspring by favoring neutrophil recruitment. PLoS Pathog 7:e1002363 [CrossRef][PubMed]
    [Google Scholar]
  20. Matsuzawa T., Fujiwara E., Washi Y. 2014; Autophagy activation by interferon-γ via the p38 mitogen-activated protein kinase signalling pathway is involved in macrophage bactericidal activity. Immunology 141:61–69 [CrossRef][PubMed]
    [Google Scholar]
  21. McDougall S., Arthur D. G., Bryan M. A., Vermunt J. J., Weir A. M. 2007; Clinical and bacteriological response to treatment of clinical mastitis with one of three intramammary antibiotics. N Z Vet J 55:161–170 [CrossRef][PubMed]
    [Google Scholar]
  22. Mutwiri G., Gerdts V., van Drunen Littel-van den Hurk S., Auray G., Eng N., Garlapati S., Babiuk L. A., Potter A. 2011; Combination adjuvants: the next generation of adjuvants?. Expert Rev Vaccines 10:95–107 [CrossRef][PubMed]
    [Google Scholar]
  23. Perez-Casal J., Prysliak T., Kerro-Dego O., Potter A. A. 2006; Immune responses to a Staphylococcus aureus GapC/B chimera and its potential use as a component of a vaccine for S. aureus mastitis. Vet Immunol Immunopathol 109:85–97 [CrossRef][PubMed]
    [Google Scholar]
  24. Prysliak T., van der Merwe J., Perez-Casal J. 2013; Vaccination with recombinant Mycoplasma bovis GAPDH results in a strong humoral immune response but does not protect feedlot cattle from an experimental challenge with M. bovis.. Microb Pathog 55:1–8 [CrossRef][PubMed]
    [Google Scholar]
  25. Shimmoto H., Kawai K., Ikawa T., Oshima S. 2010; Protection of red sea bream Pagrus major against red sea bream iridovirus infection by vaccination with a recombinant viral protein. Microbiol Immunol 54:135–142 [CrossRef][PubMed]
    [Google Scholar]
  26. Stranger-Jones Y. K., Bae T., Schneewind O. 2006; Vaccine assembly from surface proteins of Staphylococcus aureus.. Proc Natl Acad Sci U S A 103:16942–16947 [CrossRef][PubMed]
    [Google Scholar]
  27. van der Merwe J., Prysliak T., Gerdts V., Perez-Casal J. 2011; Protein chimeras containing the Mycoplasma bovis GAPDH protein and bovine host-defence peptides retain the properties of the individual components. Microb Pathog 50:269–277 [CrossRef][PubMed]
    [Google Scholar]
  28. Yu L. Q., Wang N., Ma J. Z., Tong C. Y., Song B. F., Chi J. Q., Ma G. D., Zhu Z. B., Cui Y. D. 2013; Improved protective efficacy of a chimeric Staphylococcus aureus vaccine candidate iron-regulated surface determinant B (N 126- P 361)-target of RNAIII activating protein in mice. Microbiol Immunol 57:857–864 [CrossRef][PubMed]
    [Google Scholar]
  29. Zhang P., Jespersgaard C., Lamberty-Mallory L., Katz J., Huang Y., Hajishengallis G., Michalek S. M. 2002; Enhanced immunogenicity of a genetic chimeric protein consisting of two virulence antigens of Streptococcus mutans and protection against infection. Infect Immun 70:6779–6787 [CrossRef][PubMed]
    [Google Scholar]
  30. Zhang C., Yu L., Qian R. 2007; Characterization of OmpK, GAPDH and their fusion OmpK-GAPDH derived from Vibrio harveyi outer membrane proteins: their immunoprotective ability against vibriosis in large yellow croaker (Pseudosciaena crocea). J Appl Microbiol 103:1587–1599 [CrossRef][PubMed]
    [Google Scholar]
  31. Zhao H. L., Yao X. Q., Xue C., Wang Y., Xiong X. H., Liu Z. M. 2008; Increasing the homogeneity, stability and activity of human serum albumin and interferon-alpha2b fusion protein by linker engineering. Protein Expr Purif 61:73–77 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.073593-0
Loading
/content/journal/jmm/10.1099/jmm.0.073593-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error