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Volume 63, Issue 9

Rapid identification of , and with a multiplex PCR assay Free

Abstract

, and are clinically relevant members of the (Acb) complex and important nosocomial pathogens. These three species are genetically closely related and phenotypically similar; however, they differ in their epidemiology, antibiotic resistance and pathogenicity. In this study, we investigated the use of a multiplex PCR-based assay designed to detect internal fragments of the 16S–23S rRNA intergenic region and the and genes. The assay was capable of differentiating , and in a reliable manner. In 23 different reference strains and 89 clinical isolates of species, the assay accurately identified clinically relevant Acb complex species except those ‘between 1 and 3’ or ‘close to 13TU’. None of the non-Acb complex species was misidentified. In an analysis of 1034 positive blood cultures, the assay had a sensitivity of 92.4 % and specificity of 98.2 % for Acb complex identification. Our results show that a single multiplex PCR assay can reliably differentiate clinically relevant Acb complex species. Thus, this method may be used to better understand the clinical differences between infections caused by these species.

  • Received:
  • Accepted:
  • Published Online:
© 2014 The Authors
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2014-09-01
2024-04-19
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Rapid identification of Acinetobacter baumannii, Acinetobacter nosocomialis and Acinetobacter pittii with a multiplex PCR assay
J. Med. Microbiol. 63, 1154 (2014); https://doi.org/10.1099/jmm.0.071712-0
/content/journal/jmm/10.1099/jmm.0.071712-0
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