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Journal of Medical Microbiology logo Journal of Medical Microbiology

Volume 63, Issue 5

A PCR-RFLP assay for the detection and differentiation of , , , , , and No Access

Abstract

Although and are the most common bacterial causes of human gastrointestinal diseases, other species are also involved in human and animal infections. In this study, we developed a cytolethal distending toxin () gene-based PCR-RFLP assay for the detection and differentiation of , , , , , and . Previously designed common primers, which can amplify the gene of , and , were used for detecting seven species and differentiating between them by restriction digestion. The PCR-RFLP assay was validated with 277 strains, including 35 , 19 , 20 , 24 , 13 , 2 , 22 , 3 other spp. and 17 other species associated with human diseases. Sensitivity and specificity of the PCR-RFLP assay were 100 % except for (88 % sensitivity). Furthermore, the PCR-RFLP assay successfully detected and differentiated , and in clinical and animal samples. The results indicate that the PCR-RFLP assay is useful for the detection and differentiation of seven species important for human and animal diseases.

  • Received:
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© 2014 The Authors
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/content/journal/jmm/10.1099/jmm.0.071498-0
2014-05-01
2026-03-14

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/content/journal/jmm/10.1099/jmm.0.071498-0
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A PCR-RFLP assay for the detection and differentiation of Campylobacter jejuni, C. coli, C. fetus, C. hyointestinalis, C. lari, C. helveticus and C. upsaliensis
J. Med. Microbiol. 63, 659 (2014); https://doi.org/10.1099/jmm.0.071498-0
/content/journal/jmm/10.1099/jmm.0.071498-0
/content/journal/jmm/10.1099/jmm.0.071498-0
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