1887

Abstract

Although and are the most common bacterial causes of human gastrointestinal diseases, other species are also involved in human and animal infections. In this study, we developed a cytolethal distending toxin () gene-based PCR-RFLP assay for the detection and differentiation of , , , , , and . Previously designed common primers, which can amplify the gene of , and , were used for detecting seven species and differentiating between them by restriction digestion. The PCR-RFLP assay was validated with 277 strains, including 35 , 19 , 20 , 24 , 13 , 2 , 22 , 3 other spp. and 17 other species associated with human diseases. Sensitivity and specificity of the PCR-RFLP assay were 100 % except for (88 % sensitivity). Furthermore, the PCR-RFLP assay successfully detected and differentiated , and in clinical and animal samples. The results indicate that the PCR-RFLP assay is useful for the detection and differentiation of seven species important for human and animal diseases.

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2014-05-01
2019-10-14
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