1887

Abstract

Although and are the most common bacterial causes of human gastrointestinal diseases, other species are also involved in human and animal infections. In this study, we developed a cytolethal distending toxin () gene-based PCR-RFLP assay for the detection and differentiation of , , , , , and . Previously designed common primers, which can amplify the gene of , and , were used for detecting seven species and differentiating between them by restriction digestion. The PCR-RFLP assay was validated with 277 strains, including 35 , 19 , 20 , 24 , 13 , 2 , 22 , 3 other spp. and 17 other species associated with human diseases. Sensitivity and specificity of the PCR-RFLP assay were 100 % except for (88 % sensitivity). Furthermore, the PCR-RFLP assay successfully detected and differentiated , and in clinical and animal samples. The results indicate that the PCR-RFLP assay is useful for the detection and differentiation of seven species important for human and animal diseases.

Loading

Article metrics loading...

/content/journal/jmm/10.1099/jmm.0.071498-0
2014-05-01
2020-05-26
Loading full text...

Full text loading...

/deliver/fulltext/jmm/63/5/659.html?itemId=/content/journal/jmm/10.1099/jmm.0.071498-0&mimeType=html&fmt=ahah

References

  1. Asakura M., Samosornsuk W., Taguchi M., Kobayashi K., Misawa N., Kusumoto M., Nishimura K., Matsuhisa A., Yamasaki S. 2007; Comparative analysis of cytolethal distending toxin (cdt) genes among Campylobacter jejuni, C. coli and C. fetus strains. Microb Pathog 42:174–183 [CrossRef][PubMed]
    [Google Scholar]
  2. Asakura M., Samosornsuk W., Hinenoya A., Misawa N., Nishimura K., Matsuhisa A., Yamasaki S. 2008; Development of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the detection and identification of Campylobacter jejuni, Campylobacter coli and Campylobacter fetus. FEMS Immunol Med Microbiol 52:260–266 [CrossRef][PubMed]
    [Google Scholar]
  3. Bullman S., Corcoran D., O’Leary J., O’Hare D., Lucey B., Sleator R. D. 2011; Emerging dynamics of human campylobacteriosis in Southern Ireland. FEMS Immunol Med Microbiol 63:248–253 [CrossRef][PubMed]
    [Google Scholar]
  4. Carbonero A., Torralbo A., Borge C., García-Bocanegra I., Arenas A., Perea A. 2012; Campylobacter spp., C. jejuni and C. upsaliensis infection-associated factors in healthy and ill dogs from clinics in Cordoba, Spain. Screening tests for antimicrobial susceptibility. Comp Immunol Microbiol Infect Dis 35:505–512 [CrossRef][PubMed]
    [Google Scholar]
  5. Fouts D. E., Mongodin E. F., Mandrell R. E., Miller W. G., Rasko D. A., Ravel J., Brinkac L. M., DeBoy R. T., Parker C. T.& other authors ( 2005; Major structural differences and novel potential virulence mechanisms from the genomes of multiple Campylobacter species. PLoS Biol 3:e15 [CrossRef][PubMed]
    [Google Scholar]
  6. Gharst G., Oyarzabal O. A., Hussain S. K. 2013; Review of current methodologies to isolate and identify Campylobacter spp. from foods. J Microbiol Methods 95:84–92 [CrossRef][PubMed]
    [Google Scholar]
  7. Hald B., Pedersen K., Wainø M., Jørgensen J. C., Madsen M. 2004; Longitudinal study of the excretion patterns of thermophilic Campylobacter spp. in young pet dogs in Denmark. J Clin Microbiol 42:2003–2012 [CrossRef][PubMed]
    [Google Scholar]
  8. Hoshino K., Yamasaki S., Mukhopadhyay A. K., Chakraborty S., Basu A., Bhattacharya S. K., Nair G. B., Shimada T., Takeda Y. 1998; Development and evaluation of a multiplex PCR assay for rapid detection of toxigenic Vibrio cholerae O1 and O139. FEMS Immunol Med Microbiol 20:201–207 [CrossRef][PubMed]
    [Google Scholar]
  9. Inglis G. D., Boras V. F., Houde A. 2011; Enteric campylobacteria and RNA viruses associated with healthy and diarrheic humans in the Chinook health region of southwestern Alberta, Canada. J Clin Microbiol 49:209–219 [CrossRef][PubMed]
    [Google Scholar]
  10. Kabir S. M., Kikuchi K., Asakura M., Shiramaru S., Tsuruoka N., Goto A., Hinenoya A., Yamasaki S. 2011; Evaluation of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the identification of Campylobacter strains isolated from diarrheal patients in Japan. Jpn J Infect Dis 64:19–27[PubMed]
    [Google Scholar]
  11. Lastovica A. J., le Roux E. 2000; Efficient isolation of campylobacteria from stools. J Clin Microbiol 38:2798–2799[PubMed]
    [Google Scholar]
  12. Man S. M. 2011; The clinical importance of emerging Campylobacter species. Nat Rev Gastroenterol Hepatol 8:669–685 [CrossRef][PubMed]
    [Google Scholar]
  13. Matsuda M., Shigematsu M., Tazumi A., Sekizuka T., Takamiya S., Millar B. C., Taneike I., Moore J. E. 2008; Cloning and structural analysis of the full-length cytolethal distending toxin (cdt) gene operon from Campylobacter lari. Br J Biomed Sci 65:195–199[PubMed]
    [Google Scholar]
  14. Noormohamed A., Fakhr M. K. 2012; Incidence and antimicrobial resistance profiling of Campylobacter in retail chicken livers and gizzards. Foodborne Pathog Dis 9:617–624 [CrossRef][PubMed]
    [Google Scholar]
  15. Parkhill J., Wren B. W., Mungall K., Ketley J. M., Churcher C., Basham D., Chillingworth T., Davies R. M., Feltwell T.& other authors ( 2000; The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences. Nature 403:665–668 [CrossRef][PubMed]
    [Google Scholar]
  16. Pickett C. L., Pesci E. C., Cottle D. L., Russell G., Erdem A. N., Zeytin H. 1996; Prevalence of cytolethal distending toxin production in Campylobacter jejuni and relatedness of Campylobacter sp. cdtB gene. Infect Immun 64:2070–2078[PubMed]
    [Google Scholar]
  17. Prouzet-Mauléon V., Labadi L., Bouges N., Ménard A., Mégraud F. 2006; Arcobacter butzleri: underestimated enteropathogen. Emerg Infect Dis 12:307–309 [CrossRef][PubMed]
    [Google Scholar]
  18. Samosornsuk W., Asakura M., Yoshida E., Taguchi T., Nishimura K., Eampokalap B., Phongsisay V., Chaicumpa W., Yamasaki S. 2007; Evaluation of a cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the identification of Campylobacter strains isolated from poultry in Thailand. Microbiol Immunol 51:909–917 [CrossRef][PubMed]
    [Google Scholar]
  19. Sandstedt K., Ursing J. 1991; Description of Campylobacter upsaliensis sp. nov. previously known as the CNW group. Syst Appl Microbiol 14:39–45 [CrossRef]
    [Google Scholar]
  20. Shiramaru S., Asakura M., Inoue H., Nagita A., Matsuhisa A., Yamasaki S. 2012; A cytolethal distending toxin gene-based multiplex PCR assay for detection of Campylobacter spp. in stool specimens and comparison with culture method. J Vet Med Sci 74:857–862 [CrossRef][PubMed]
    [Google Scholar]
  21. Sivadon-Tardy V., Porcher R., Orlikowski D., Ronco E., Gault E., Roussi J., Durand M. C., Sharshar T., Annane D.& other authors ( 2013; Increased incidence of Campylobacter jejuni-associated Guillain-Barré syndromes in the Greater Paris area. Epidemiol Infect 10:1–5 [CrossRef][PubMed]
    [Google Scholar]
  22. Stanley J., Burnens A. P., Linton D., On S. L., Costas M., Owen R. J. 1992; Campylobacter helveticus sp. nov., a new thermophilic species from domestic animals: characterization, and cloning of a species-specific DNA probe. J Gen Microbiol 138:2293–2303 [CrossRef][PubMed]
    [Google Scholar]
  23. Steinhauserova I., Fojtikova K., Klimes J. 2000; The incidence and PCR detection of Campylobacter upsaliensis in dogs and cats. Lett Appl Microbiol 31:209–212 [CrossRef][PubMed]
    [Google Scholar]
  24. Vandenberg O., Dediste A., Houf K., Ibekwem S., Souayah H., Cadranel S., Douat N., Zissis G., Butzler J. P., Vandamme P. 2004; Arcobacter species in humans. Emerg Infect Dis 10:1863–1867 [CrossRef][PubMed]
    [Google Scholar]
  25. Yamasaki S., Asakura M., Tsukamoto T., Faruque S. M., Deb R., Ramamurthy T. 2006; Cytolethal distending toxin (CDT): genetic diversity, structure and role in diarrheal disease. Toxin Reviews 25:61–88 [CrossRef]
    [Google Scholar]
  26. Young K. T., Davis L. M., Dirita V. J. 2007; Campylobacter jejuni: molecular biology and pathogenesis. Nat Rev Microbiol 5:665–679 [CrossRef][PubMed]
    [Google Scholar]
  27. Yuki N., Hartung H. P. 2012; Guillain-Barré syndrome. N Engl J Med 366:2294–2304 [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jmm/10.1099/jmm.0.071498-0
Loading
/content/journal/jmm/10.1099/jmm.0.071498-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error