1887

Abstract

Antibiotic resistance is increasing worldwide, and it has been regarded as the main factor reducing the efficacy of therapy. The aim of this study was to determine the phenotype and genotype of antibiotic-resistant strains of in the Malaysian population and to evaluate the impact of antibiotic resistance to eradication outcome. One hundred and sixty-one isolates were analysed in this study. Metronidazole, clarithromycin, fluoroquinolone, amoxicillin and tetracycline susceptibilities were determined by Etest. PCR followed by DNA sequencing was carried out to determine mutations. The medical records of the patients infected with resistant strains were reviewed to determine the eradication outcome. Metronidazole resistance was encountered in 36.6 % of isolates, whereas clarithromycin and fluoroquinolone resistance was observed in 1.2  and 1.9 % of isolates, respectively. All strains tested were susceptible to amoxicillin and tetracycline. Frameshift and nonsense mutations in and genes resulting in stop codons contributed to metronidazole resistance, which leads to reduced eradication efficacy. A2142G and A2143G mutations of 23S rRNA were identified as causing failure of the eradication therapy. Mutation at either codon 87 or 91 of the gene was identified in fluoroquinolone-resistant strains. However, the effect of resistance could not be assessed. This study showed that frameshift and nonsense mutations in or genes and point mutations in the 23S rRNA affected the efficacy of eradication therapy.

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2014-05-01
2019-12-09
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