1887

Abstract

The aim of this work was to evaluate the effects of beneficial human vaginal lactobacilli (Lb) on urogenital pathogens through and experiments. Co-aggregative and antimicrobial properties between five vaginal Lb strains and urogenital pathogens or potential pathogens (, and strains) were assayed. Also, associative cultures of Lb strains and were performed and bacterial growth, pH, lactic acid and hydrogen peroxide (HO) were determined at different times. Based on the results obtained, the studies were assayed in mice with CRL 1509 or CRL 1328 inoculated intravaginally (i.v.) and then challenged i.v. with . Results were analysed by ANOVA (repeated measures and general linear models). Most of the Lb strains increased the percentage of aggregation of strains. Only one strain ( CRL 1324) positively affected the aggregation of and none increased the aggregation of . The inhibition of the growth of strains by production of organic acids by lactobacilli was evidenced. The Lb– co-cultures showed a significant inhibition of the pathogen after 4 h and 8 h of incubation. Parallel increases in lactic acid and HO levels were observed. However, in the experimental murine model, no significant differences were obtained in the number of streptococci recovered from the vaginal tract of control mice and those inoculated with Lb. In conclusion, vaginal Lb exhibited co-aggregative and antimicrobial effects on strains, suggesting that they could be promising candidates for protection against challenge. However, as these effects were not evidenced in the murine model used, further animal studies under different experimental conditions should be conducted to evaluate the preventive effect of Lb against challenge with .

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2014-05-01
2024-03-19
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