Clinical utility of loop-mediated isothermal amplification for rapid diagnosis of in children Free

Abstract

Loop-mediated isothermal amplification (LAMP) is a cost-effective and rapid method for identifying (MP). We investigated the utility of the LAMP assay in diagnosing MP pneumonia among children in a clinical setting. In this prospective study, the cause of community-acquired pneumonia was evaluated in 111 patients for whom MP was the suspected pathogen. All participants were patients at a city hospital in Japan between April 2012 and September 2012. Throat swabs for the LAMP assay were obtained at admission, and paired serum samples to measure antibody titres to MP by particle agglutination were obtained at admission and during convalescence. Overall, 45 of 111 (41 %) patients had a fourfold or greater increase in MP titres and received a diagnosis of MP pneumonia. Among them, 43 (96 %) patients (median age, 9 years) were positive on the LAMP assay and had a fourfold or greater increase in MP titres. The median interval from fever onset to collection of throat swabs was 7 days (range, 4–10 days). As compared with paired serum titres, the LAMP assay enabled quicker diagnosis of MP (median interval, 13 vs. 7 days), thereby allowing early initiation of appropriate antimicrobial therapy.

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2014-02-01
2024-03-28
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References

  1. Aryan E., Makvandi M., Farajzadeh A., Huygen K., Alvandi A. H., Gouya M. M., Sadrizadeh A., Romano M. 2013; Clinical value of IS6110-based loop-mediated isothermal amplification for detection of Mycobacterium tuberculosis complex in respiratory specimens. J Infect 66:487–493 [View Article][PubMed]
    [Google Scholar]
  2. Daxboeck F., Krause R., Wenisch C. 2003; Laboratory diagnosis of Mycoplasma pneumoniae infection. Clin Microbiol Infect 9:263–273 [View Article][PubMed]
    [Google Scholar]
  3. Ferwerda A., Moll H. A., de Groot R. 2001; Respiratory tract infections by Mycoplasma pneumoniae in children: a review of diagnostic and therapeutic measures. Eur J Pediatr 160:483–491 [View Article][PubMed]
    [Google Scholar]
  4. Gotoh K., Nishimura N., Ohshima Y., Arakawa Y., Hosono H., Yamamoto Y., Iwata Y., Nakane K., Funahashi K., Ozaki T. 2012; Detection of Mycoplasma pneumoniae by loop-mediated isothermal amplification (LAMP) assay and serology in pediatric community-acquired pneumonia. J Infect Chemother 18:662–667 [View Article][PubMed]
    [Google Scholar]
  5. Kamachi K., Toyoizumi-Ajisaka H., Toda K., Soeung S. C., Sarath S., Nareth Y., Horiuchi Y., Kojima K., Takahashi M., Arakawa Y. 2006; Development and evaluation of a loop-mediated isothermal amplification method for rapid diagnosis of Bordetella pertussis infection. J Clin Microbiol 44:1899–1902 [View Article][PubMed]
    [Google Scholar]
  6. Kliegman R. 2011 Nelson Textbook of Pediatrics, 19th edn. vol. 994 Philadelphia: Elsevier Saunders;
    [Google Scholar]
  7. Korppi M., Heiskanen-Kosma T., Kleemola M. 2004; Incidence of community-acquired pneumonia in children caused by Mycoplasma pneumoniae: serological results of a prospective, population-based study in primary health care. Respirology 9:109–114 [View Article][PubMed]
    [Google Scholar]
  8. Kuroki H., Morozumi M., Chiba N., Ubukata K. 2004; Characterization of children with Mycoplasma pneumoniae infection detected by rapid polymerase chain reaction technique. J Infect Chemother 10:65–67 [View Article][PubMed]
    [Google Scholar]
  9. Long S. S., Pickering L. K., Prober C. G. 2012 Principles and Practice of Pediatric Infectious Diseases, 4th edn. London: Elsevier Churchill Livingstone;
    [Google Scholar]
  10. Michelow I. C., Olsen K., Lozano J., Duffy L. B., McCracken G. H., Hardy R. D. 2004; Diagnostic utility and clinical significance of naso- and oropharyngeal samples used in a PCR assay to diagnose Mycoplasma pneumoniae infection in children with community-acquired pneumonia. J Clin Microbiol 42:3339–3341 [View Article][PubMed]
    [Google Scholar]
  11. Mori Y., Nagamine K., Tomita N., Notomi T. 2001; Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem Biophys Res Commun 289:150–154 [View Article][PubMed]
    [Google Scholar]
  12. Morozumi M., Nakayama E., Iwata S., Aoki Y., Hasegawa K., Kobayashi R., Chiba N., Tajima T., Ubukata K. 2006; Simultaneous detection of pathogens in clinical samples from patients with community-acquired pneumonia by real-time PCR with pathogen-specific molecular beacon probes. J Clin Microbiol 44:1440–1446 [View Article][PubMed]
    [Google Scholar]
  13. Morozumi M., Takahashi T., Ubukata K. 2010; Macrolide-resistant Mycoplasma pneumoniae: characteristics of isolates and clinical aspects of community-acquired pneumonia. J Infect Chemother 16:78–86 [View Article][PubMed]
    [Google Scholar]
  14. Nir-Paz R., Michael-Gayego A., Ron M., Block C. 2006; Evaluation of eight commercial tests for Mycoplasma pneumoniae antibodies in the absence of acute infection. Clin Microbiol Infect 12:685–688 [View Article][PubMed]
    [Google Scholar]
  15. Notomi T., Okayama H., Masubuchi H., Yonekawa T., Watanabe K., Amino N., Hase T. 2000; Loop-mediated isothermal amplification of DNA. Nucleic Acids Res 28:e63 [View Article][PubMed]
    [Google Scholar]
  16. Saito R., Misawa Y., Moriya K., Koike K., Ubukata K., Okamura N. 2005; Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of Mycoplasma pneumoniae. J Med Microbiol 54:1037–1041 [View Article][PubMed]
    [Google Scholar]
  17. Sidoti F., Bergallo M., Costa C., Cavallo R. 2013; Alternative molecular tests for virological diagnosis. Mol Biotechnol 53:352–362 [View Article][PubMed]
    [Google Scholar]
  18. Silvia B., Loreto F., Albert B., Cristina P., Aida R., Lurdes M., Carlos R., Vicente A. 2011; Comparison of 2 molecular assays and a serologic test in diagnosing Mycoplasma pneumoniae infection in paediatrics patients. Diagn Microbiol Infect Dis 71:463–466 [View Article][PubMed]
    [Google Scholar]
  19. Waites K. B. 2003; New concepts of Mycoplasma pneumoniae infections in children. Pediatr Pulmonol 36:267–278 [View Article][PubMed]
    [Google Scholar]
  20. Yoshino M., Annaka T., Kojima T., Ikedo M. 2008; [Sensitive and rapid detection of Mycoplasma pneumoniae by loop-mediated isothermal amplification]. Kansenshogaku Zasshi 82:168–176 (in Japanese) [View Article][PubMed]
    [Google Scholar]
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