1887

Abstract

Multilocus sequence typing and antimicrobial susceptibility testing were performed for three community-onset New Delhi metallo-β-lactamase-1 (NDM-1)-producing isolates from Korea. The genetic structure surrounding the gene was determined in -harbouring plasmids. Three NDM-1-producing isolates were found to belong to the same clone (sequence type 340). Each of these isolates showed the same genetic structure surrounding the gene. The genes , , and were flanked by two intact insertion sequences, IS and IS, which may promote horizontal gene transfer. The -harbouring plasmids conferred antimicrobial resistance to carbapenems, cephalosporins, aminoglycosides and aztreonam in transconjugants. It can be speculated that either the entire -harbouring plasmids or just the part of the plasmid containing the gene may have transferred between and . Following the transfer, the isolate disseminated throughout Korea. This study suggests the need for monitoring the dissemination of NDM-1-producing isolates across countries or continents due to their potential transferability via IS and IS-associated transposons.

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2014-01-01
2019-10-19
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References

  1. Bushnell G., Mitrani-Gold F., Mundy L. M.. ( 2013;). Emergence of New Delhi metallo-β-lactamase type 1-producing Enterobacteriaceae and non-Enterobacteriaceae: global case detection and bacterial surveillance. . Int J Infect Dis 17:, e325–e333. [CrossRef][PubMed]
    [Google Scholar]
  2. Cardoso O., Alves A. F., Leitão R.. ( 2008;). Metallo-β-lactamase VIM-2 in Pseudomonas aeruginosa isolates from a cystic fibrosis patient. . Int J Antimicrob Agents 31:, 375–379. [CrossRef][PubMed]
    [Google Scholar]
  3. CLSI ( 2013;). Performance Standards for Antimicrobial Susceptibility Testing; 21st Informational Supplement, M100-S23. Wayne, PA:: Clinical and Laboratory Standards Institute;.
    [Google Scholar]
  4. Diancourt L., Passet V., Verhoef J., Grimont P. A., Brisse S.. ( 2005;). Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates. . J Clin Microbiol 43:, 4178–4182. [CrossRef][PubMed]
    [Google Scholar]
  5. Ho P. L., Lo W. U., Yeung M. K., Lin C. H., Chow K. H., Ang I., Tong A. H. Y., Bao J. Y. J., Lok S., Lo J. Y. C.. ( 2011;). Complete sequencing of pNDM-HK encoding NDM-1 carbapenemase from a multidrug-resistant Escherichia coli strain isolated in Hong Kong. . PLoS ONE 6:, e17989. [CrossRef][PubMed]
    [Google Scholar]
  6. Kim M. N., Yong D., An D., Chung H. S., Woo J. H., Lee K., Chong Y.. ( 2012;). Nosocomial clustering of NDM-1-producing Klebsiella pneumoniae sequence type 340 strains in four patients at a South Korean tertiary care hospital. . J Clin Microbiol 50:, 1433–1436. [CrossRef][PubMed]
    [Google Scholar]
  7. Kim S. Y., Shin J., Shin S. Y., Ko K. S.. ( 2013;). Characteristics of carbapenem-resistant Enterobacteriaceae isolates from Korea. . Diagn Microbiol Infect Dis 76:, 486–490. [CrossRef][PubMed]
    [Google Scholar]
  8. Kumarasamy K. K., Toleman M. A., Walsh T. R., Bagaria J., Butt F., Balakrishnan R., Chaudhary U., Doumith M., Giske C. G.. & other authors ( 2010;). Emergence of a new antibiotic resistance mechanism in India, Pakistan, and the UK: a molecular, biological, and epidemiological study. . Lancet Infect Dis 10:, 597–602. [CrossRef][PubMed]
    [Google Scholar]
  9. Liu Z., Li W., Wang J., Pan J., Sun S., Yu Y., Zhao B., Ma Y., Zhang T.. & other authors ( 2013;). Identification and characterization of the first Escherichia coli strain carrying NDM-1 gene in China. . PLoS ONE 8:, e66666. [CrossRef][PubMed]
    [Google Scholar]
  10. Nordmann P., Poirel L., Walsh T. R., Livermore D. M.. ( 2011;). The emerging NDM carbapenemases. . Trends Microbiol 19:, 588–595. [CrossRef][PubMed]
    [Google Scholar]
  11. Peirano G., Pillai D. R., Pitondo-Silva A., Richardson D., Pitout J. D.. ( 2011;). The characteristics of NDM-producing Klebsiella pneumoniae from Canada. . Diagn Microbiol Infect Dis 71:, 106–109. [CrossRef][PubMed]
    [Google Scholar]
  12. Poirel L., Al Maskari Z., Al Rashdi F., Bernabeu S., Nordmann P.. ( 2011;). NDM-1-producing Klebsiella pneumoniae isolated in the Sultanate of Oman. . J Antimicrob Chemother 66:, 304–306. [CrossRef][PubMed]
    [Google Scholar]
  13. Shin J., Choi M. J., Ko K. S.. ( 2012;). Replicon sequence typing of IncF plasmids and the genetic environments of blaCTX-M-15 indicate multiple acquisitions of blaCTX-M-15 in Escherichia coli and Klebsiella pneumoniae isolates from South Korea. . J Antimicrob Chemother 67:, 1853–1857. [CrossRef][PubMed]
    [Google Scholar]
  14. Solé M., Pitart C., Roca I., Fàbrega A., Salvador P., Muñoz L., Oliveira I., Gascón J., Marco F., Vila J.. ( 2011;). First description of an Escherichia coli strain producing NDM-1 carbapenemase in Spain. . Antimicrob Agents Chemother 55:, 4402–4404. [CrossRef][PubMed]
    [Google Scholar]
  15. Villa L., Poirel L., Nordmann P., Carta C., Carattoli A.. ( 2012;). Complete sequencing of an IncH plasmid carrying the blaNDM-1, blaCTX-M-15 and qnrB1 genes. . J Antimicrob Chemother 67:, 1645–1650. [CrossRef][PubMed]
    [Google Scholar]
  16. Walsh T. R., Weeks J., Livermore D. M., Toleman M. A.. ( 2011;). Dissemination of NDM-1 positive bacteria in the New Delhi environment and its implications for human health: an environmental point prevalence study. . Lancet Infect Dis 11:, 355–362. [CrossRef][PubMed]
    [Google Scholar]
  17. Yong D., Toleman M. A., Giske C. G., Cho H. S., Sundman K., Lee K., Walsh T. R.. ( 2009;). Characterization of a new metallo-beta-lactamase gene, bla(NDM-1), and a novel erythromycin esterase gene carried on a unique genetic structure in Klebsiella pneumoniae sequence type 14 from India. . Antimicrob Agents Chemother 53:, 5046–5054. [CrossRef][PubMed]
    [Google Scholar]
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