%0 Journal Article %A Alvargonzalez, Jorge Julio Cabrera %A Hernando, Ana Vindel %A Martín, Maria Dolores Rojo %A Casas, Consuelo Miranda %A Iglesias, Jesús Oteo %A Marín, Mari Fe Bautista %A Alvarez, Maria Luisa Azañedo %A Sanchez, Veronica Bautista %A Marí, Jose Maria Navarro %T Sequential outbreaks in a Spanish hospital caused by multiresistant OXA-58-producing Acinetobacter baumannii ST92 %D 2014 %J Journal of Medical Microbiology, %V 63 %N 8 %P 1093-1098 %@ 1473-5644 %R https://doi.org/10.1099/jmm.0.067280-0 %I Microbiology Society, %X The aim of this study was to assess the epidemiology and molecular basis of the infection and dissemination of multidrug-resistant Acinetobacter baumannii (MDRAB) in three sequential outbreaks at the intensive care units (ICUs) of a tertiary university hospital in Granada, Spain, between 2009 and 2011. Strains from all patients infected and/or colonized by MDRAB during outbreak periods were characterized using PFGE and multilocus sequence typing (MLST). The first outbreak appeared in the summer of 2009 involving 38 ICU patients: 25 from a Traumatology–Rehabilitation hospital (TRH) and 13 from a Medical–Surgery hospital (MSH). Between 2010 and 2011, outbreaks were limited to the MSH-ICU, affecting 9 and 11 patients, respectively. Two PFGE types were detected. In the 2009 outbreak, two clones were identified: profile 1 strains were isolated at the TRH, whilst profile 2 was isolated at the MSH. Only one clone was identified in the 2010 and 2011 outbreaks: the profile 2 clone detected at the MSH in 2009. After MLST analysis, a single sequence type (ST92) was identified. This suggested that an endemic strain could evolve and cause localized outbreaks in vulnerable patients. Multiplex PCR for OXA group enzymes yielded a positive result for bla OXA-58-like and bla OXA-51-like genes, and gene sequencing showed the presence of bla OXA-58. However, the absence of ISAba1 upstream of the bla OXA-51-like gene suggested the absence of OXA-51 expression. The susceptibility pattern was not an appropriate method for MDRAB surveillance, as several susceptibility patterns were identified in a single clone. Consequently, molecular methods of characterization are recommended for epidemiological surveillance of MDRAB. %U https://www.microbiologyresearch.org/content/journal/jmm/10.1099/jmm.0.067280-0