1887

Abstract

The aminoglycoside phosphotransferase primarily inactivates kanamycin and neomycin, whilst also inactivates amikacin. The aim of this study was to determine the frequency of both resistance genes in major human pathogens to obtain their baseline prevalence in the gene pool of these bacterial populations in Austria. In total, 10 541 subsp. and isolates were collected representatively without selection bias between 2008 and 2011. Isolates were analysed by - and -specific TaqMan real-time PCR. For positive strains, MICs using Etests were performed and resistance gene sequences were determined. The overall prevalence of was 1.62 % (95 % confidence interval: 1.38–1.88 %). In , enterococci, and spp., the prevalence was 0.47 % (0–1.47 %), 37.53 % (32.84–42.40 %), 2.90 % (1.51–5.02 %), 0 % (0–0.32 %) and 0 % (0–0.037 %), respectively. Eleven of a total of 169 carriers showed single-nucleotide polymorphisms in the resistance allele. The overall prevalence of was 0.0096 % (0–0.046 %). (0–0.70 %), enterococci (0–0.75 %), (0–0.73 %) and (0–0.32 %) did not carry . A single isolate was positive, resulting in an prevalence of 0.013 % (0–0.058 %). carriers were moderately prevalent in the strains tested except for in enterococci, which appeared to be an important reservoir for . genes were detected at clinically irrelevant frequencies and played no significant role in the aminoglycoside resistance gene pool during the observation period.

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2014-02-01
2020-01-20
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