1887

Abstract

A total of 15 adult ewes from one flock known to be seroreactive for leptospirosis was studied. Urine and vaginal fluid were collected from each animal to test for the presence of leptospires using bacterial culture and conventional PCR methods. One pure culture of sp. was obtained from the vaginal fluid sample of a non-pregnant ewe. The isolate was characterized by DNA sequencing of the and genes, variable-number of tandem-repeats (VNTR) analysis and serogrouping, and the isolate was typed as serogroup Sejroe serovar Hardjo type Hardjoprajitno. This report indicates the presence of viable in the vaginal fluid of a ewe, suggesting the potential for venereal transmission of leptospires in sheep.

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2014-09-01
2019-11-12
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References

  1. Cerri D., Nuvoloni R., Ebani V., Pedrini A., Mani P., Andreani E., Farina R.. ( 1996;). Leptospira interrogans serovar Hardjo in the kidneys and genital tracts of naturally infected sheep. . New Microbiol 19:, 175–178.[PubMed]
    [Google Scholar]
  2. Cosate M. R., Barouni A. S., Moreira E. C., Veloso I. F., Gomes M. T., Salas C. E.. ( 2012;). Molecular characterization by LSSP-PCR and DNA sequencing of a pathogenic isolate of Leptospira interrogans from Brazil. . Zoonoses Public Health 59:, 379–388. [CrossRef][PubMed]
    [Google Scholar]
  3. Dhaliwal G. S., Murray R. D., Dobson H., Montgomery J., Ellis W. A.. ( 1996;). Presence of antigen and antibodies in serum and genital discharges of cows from dairy herds naturally infected with Leptospira interrogans serovar Hardjo. . Res Vet Sci 60:, 163–167. [CrossRef][PubMed]
    [Google Scholar]
  4. Dorjee S., Heuer C., Jackson R., West D. M., Collins-Emerson J. M., Midwinter A. C., Ridler A. L.. ( 2009;). Are white-spot lesions in kidneys in sheep associated with leptospirosis?. N Z Vet J 57:, 28–33. [CrossRef][PubMed]
    [Google Scholar]
  5. Ellis W. A., Thiermann A. B.. ( 1986;). Isolation of leptospires from the genital tracts of Iowa cows. . Am J Vet Res 47:, 1694–1696.[PubMed]
    [Google Scholar]
  6. Ellis W. A., Songer J. G., Montgomery J., Cassells J. A.. ( 1986;). Prevalence of Leptospira interrogans serovar Hardjo in the genital and urinary tracts of non-pregnant cattle. . Vet Rec 118:, 11–13. [CrossRef][PubMed]
    [Google Scholar]
  7. Gerritsen M. J., Koopmans M. J., Peterse D., Olyhoek T.. ( 1994;). Sheep as maintenance host for Leptospira interrogans serovar Hardjo subtype Hardjobovis. . Am J Vet Res 55:, 1232–1237.[PubMed]
    [Google Scholar]
  8. Hamond C., Martins G., Lilenbaum W., Medeiros M. A.. ( 2012;). PCR detection of leptospiral carriers among seronegative horses. . Vet Rec 171:, 105–106. [CrossRef][PubMed]
    [Google Scholar]
  9. Hamond C., Martins G., Loureiro A. P., Pestana C., Lawson-Ferreira R., Medeiros M. A., Lilenbaum W.. ( 2014;). Urinary PCR as an increasingly useful tool for an accurate diagnosis of leptospirosis in livestock. . Vet Res Commun 38:, 81–85. [CrossRef][PubMed]
    [Google Scholar]
  10. Hartskeerl R. A., Collares-Pereira M., Ellis W. A.. ( 2011;). Emergence, control and re-emerging leptospirosis: dynamics of infection in the changing world. . Clin Microbiol Infect 17:, 494–501. [CrossRef][PubMed]
    [Google Scholar]
  11. Kingscote B.. ( 1985;). Leptospirosis in sheep in Western Canada. . Can Vet J 26:, 164–168.[PubMed]
    [Google Scholar]
  12. Lilenbaum W., Varges R., Brandão F. Z., Cortez A., de Souza S. O., Brandão P. E., Richtzenhain L. J., Vasconcellos S. A.. ( 2008;). Detection of Leptospira spp. in semen and vaginal fluids of goats and sheep by polymerase chain reaction. . Theriogenology 69:, 837–842. [CrossRef][PubMed]
    [Google Scholar]
  13. Magajevski F. S., Girio R. J. S., Mathias L. A., Myashiro S., Genovez M. É., Scarcelli E. P.. ( 2005;). Detection of Leptospira spp. In the semen and urine off bulls serologically reactive to Leptospira interrogans serovar Hardjo. . Braz J Microbiol 36:, 43–45. [CrossRef]
    [Google Scholar]
  14. Majed Z., Bellenger E., Postic D., Pourcel C., Baranton G., Picardeau M.. ( 2005;). Identification of variable-number tandem-repeat loci in Leptospira interrogans sensu stricto.. J Clin Microbiol 43:, 539–545. [CrossRef][PubMed]
    [Google Scholar]
  15. Martins G., Penna B., Hamond C., Leite R. C., Silva A., Ferreira A., Brandão F., Oliveira F., Lilenbaum W.. ( 2012;). Leptospirosis as the most frequent infectious disease impairing productivity in small ruminants in Rio de Janeiro, Brazil. . Trop Anim Health Prod 44:, 773–777. [CrossRef][PubMed]
    [Google Scholar]
  16. Otaka D. Y., Martins G., Hamond C., Penna B., Medeiros M. A., Lilenbaum W.. ( 2012;). Serology and PCR for bovine leptospirosis: herd and individual approaches. . Vet Rec 170:, 338. [CrossRef][PubMed]
    [Google Scholar]
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