1887

Abstract

infection has been shown to be associated with the development of atherosclerosis by promoting the migration of vascular smooth muscle cells (VSMCs). However, how infection induces VSMC migration is not fully understood. A primary role of Ras-related C3 botulinum toxin substrate 1 (Rac1) is to generate a protrusive force at the leading edge that contributes to cell migration. Whether Rac1 activation plays a role in infection-induced VSMC migration is not well defined. In the present study, we therefore examined Rac1 activation in infected rat primary VSMCs and the role of Rac1 activation in infection-induced VSMC migration. Glutathione -transferase pull-down assay results showed that Rac1 was activated in -infected rat primary VSMCs. A Rac1 inhibitor, NSC23766 (50 µM,) suppressed Rac1 activation stimulated by infection, and thereby inhibited infection-induced VSMC migration. In addition, infection-induced Rac1 activation in the VSMCs was blocked by LY294002 (25 µM), an inhibitor of phosphatidylinositol 3-kinase (PI3K). Taken together, these data suggest that infection promotes VSMC migration, possibly through activating Rac1 via PI3K.

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2014-02-01
2019-10-15
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