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Abstract

The aims of this study were to (1) conduct a national survey of identification by National Neisseria Network (NNN) reference laboratories contributing data to the Australian Gonococcal Surveillance Programme and (2) determine the prevalence in Australia of strains of lacking gene sequences commonly targeted by in-house PCR assays for confirmation of gonococcal nucleic acid amplification tests. Gonococcal clinical isolates referred to NNN laboratories for the first half of 2012 were screened using in-house real-time PCR assays targeting multicopy , pseudogene and genes. There were 2455 clinical gonococcal isolates received in the study period; 98.6 % (2420/2455) of isolates harboured all three gene targets, 0.12 % (3/2455) were -negative, 0.04 % (1/2455) -negative and 1.14 % (28/2455) -negative by PCR. Notably, no isolates were simultaneously negative for two targets. However, three isolates failed to be amplified by all three PCR methods, one isolate of which was shown to be a commensal strain by 16S rRNA sequencing. Using PCR as the reference standard the results showed that (1) identification of isolates by NNN laboratories was highly specific (99.96 %) and (2) strains of lacking gene sequences commonly targeted by in-house PCR assays are present but not widespread throughout Australia at this point in time.

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2014-01-01
2024-03-29
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