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Journal of Medical Microbiology logo

Volume 62, Issue 11

Molecular diagnosis of bloodstream infections with a new dual-priming oligonucleotide-based multiplex PCR assay Free

Abstract

Mortality from bloodstream infections (BSIs) correlates with diagnostic delay and the use of inappropriate empirical treatment. Early PCR-based diagnosis could decrease inappropriate treatment, improving patient outcome. The aim of the present study was to assess the clinical utility of this molecular technology to diagnose BSIs. We assessed a new dual-priming oligonucleotide-based multiplex PCR assay, the Magicplex Sepsis Test (MST) (Seegene), along with blood culture (BC). A total of 267 patients from the intensive care unit and haematology and emergency departments were enrolled. Clinical data were also used by physicians to determine the likelihood of infection. Ninety-eight (37 %) specimens were positive: 29 (11 %) by both the MST and BC, 29 (11 %) by the MST only, and 40 (15 %) by BC only. The proportion of agreement between the two methods was 73 % (Cohen’s κ: 0.45; 0.28–0.6; indicating fair to moderate agreement). According to clinical assessment, 63 (64 %) positive specimens were considered BSIs: 23 (36 %) were positive by both the MST and BC, 22 (35 %) were positive only by BC, and 18 (29 %) were positive only by the MST. Thirty-eight (14 %) positive specimens by the MST and/or BC were considered as contaminants. Of 101 specimens collected from patients receiving antibiotics, 20 (20 %) were positive by the MST and 32 (32 %) by BC. Sensitivity and specificity were 65 % and 92 %, respectively, for the MST and 71 % and 88 %, respectively for BC. We concluded that the MST shows a high specificity but changes in design are needed to increase bacteraemia detection. For viability in clinical laboratories, technical improvements are also required to further automate the process.

  • Received:
  • Accepted:
  • Published Online:
© 2013 SGM
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2013-11-01
2024-04-19
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Molecular diagnosis of bloodstream infections with a new dual-priming oligonucleotide-based multiplex PCR assay
J. Med. Microbiol. 62, 1673 (2013); https://doi.org/10.1099/jmm.0.064758-0
/content/journal/jmm/10.1099/jmm.0.064758-0
/content/journal/jmm/10.1099/jmm.0.064758-0
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