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Volume 63, Issue 3

Frequency and antimicrobial resistance of diarrhoeagenic from young children in Iran Free

Abstract

Diarrhoea continues to be one of the most common causes of morbidity and mortality among infants and children in developing countries. Diarrhoeagenic (DEC) is an emerging agent among pathogens that cause diarrhoea. Between March 2011 and January 2012, a total of 600 stool specimens from children younger than 5 years of age (450 with and 150 without diarrhoea) were investigated for enteroaggregative (EAEC), enterohaemorrhagic (EHEC), enteropathogenic (EPEC) and enterotoxigenic (ETEC) using PCR. Antimicrobial susceptibility testing was performed following Clinical and Laboratory Standards Institute guidelines. The prevalence of DEC pathotypes was 30.4 % (137 patients) and 12 % (18 patients) in the diarrhoea group and the control group, respectively. The most frequently isolated pathotype in diarrhoeal children was ETEC. This pathotype was detected significantly more often in children with diarrhoea (14.4 %) than in children without diarrhoea (5.3 %). EAEC and EPEC were detected with slightly higher frequencies in children with (8 and 4.2 %, respectively) than in children without (4.6 and 2 %, respectively) (>0.05) diarrhoea. EHEC was only detected in children with diarrhoea (3.8 %). Of the children from the diarrhoea group, 10 % were colonized with more than one DEC pathotype. The DEC isolates exhibited high-level resistance to erythromycin (100 %), azteronam (80.7 %), amoxicillin (74.4 %) and tetracycline (69.3 %), and 86.4 % of isolates were multidrug resistant. In conclusion, ETEC continues to be an important agent associated with diarrhoea in children from Tabriz, Iran.

  • Received:
  • Accepted:
  • Published Online:
© 2014 SGM
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2014-03-01
2024-04-20
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References

  1. Aidar-Ugrinovich L., Blanco J., Blanco M., Blanco J. E., Leomil L., Dahbi G., Mora A., Onuma D. L., Silveira W. D., Pestana de Castro A. F. 2007; Serotypes, virulence genes, and intimin types of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) isolated from calves in São Paulo, Brazil. Int J Food Microbiol 115:297–306 [View Article][PubMed]
     [Google Scholar]
  2. Al-Gallas N., Bahri O., Bouratbeen A., Ben Haasen A., Ben Aissa R. 2007; Etiology of acute diarrhea in children and adults in Tunis, Tunisia, with emphasis on diarrheagenic Escherichia coli: prevalence, phenotyping, and molecular epidemiology. Am J Trop Med Hyg 77:571–582[PubMed]
     [Google Scholar]
  3. Aranda K. R. S., Fagundes-Neto U., Scaletsky I. C. A. 2004; Evaluation of multiplex PCRs for diagnosis of infection with diarrheagenic Escherichia coli and Shigella spp. J Clin Microbiol 42:5849–5853 [View Article][PubMed]
     [Google Scholar]
  4. Aslani M. M., Alikhani M. Y., Zavari A., Yousefi R., Zamani A. R. 2011; Characterization of enteroaggregative Escherichia coli (EAEC) clinical isolates and their antibiotic resistance pattern. Int J Infect Dis 15:e136–e139 [View Article][PubMed]
     [Google Scholar]
  5. Boschi-Pinto C., Velebit L., Shibuya K. 2008; Estimating child mortality due to diarrhoea in developing countries. Bull World Health Organ 86:710–717 [View Article][PubMed]
     [Google Scholar]
  6. Bueris V., Sircili M. P., Taddei C. R., dos Santos M. F., Franzolin M. R., Martinez M. B., Ferrer S. R., Barreto M. L., Trabulsi L. R. 2007; Detection of diarrheagenic Escherichia coli from children with and without diarrhea in Salvador, Bahia, Brazil. Mem Inst Oswaldo Cruz 102:839–844 [View Article][PubMed]
     [Google Scholar]
  7. De Baets L., Van der Taelen I., De Filette M., Piérard D., Allison L., De Greve H., Hernalsteens J.-P., Imberechts H. 2004; Genetic typing of Shiga toxin 2 variants of Escherichia coli by PCR-restriction fragment length polymorphism analysis. Appl Environ Microbiol 70:6309–6314 [View Article][PubMed]
     [Google Scholar]
  8. El-Metwally H. A. R., Ibrahim H. A. H., El-Athamna M. N., Amer M. A. 2007; Multiplex PCR for detection of diarrheagenic E. coli in Egyptian children. J Med Sci 7:255–262 [View Article]
     [Google Scholar]
  9. Fujihara S., Arikawa K., Aota T., Tanaka H., Nakamura H., Wada T., Hase A., Nishikaw Y. 2009; Prevalence and properties of diarrheagenic Escherichia coli among healthy individuals in Osaka City, Japan. Jpn J Infect Dis 62:318–323[PubMed]
     [Google Scholar]
  10. Kagambèga A., Martikainen O., Lienemann T., Siitonen A., Traoré A. S., Barro N., Haukka K. 2012; Diarrheagenic Escherichia coli detected by 16-plex PCR in raw meat and beef intestines sold at local markets in Ouagadougou, Burkina Faso. Int J Food Microbiol 153:154–158 [View Article][PubMed]
     [Google Scholar]
  11. Mayatepek E., Seebass E., Hingst V., Kroeger A., Sonntag H. G. 1993; Prevalence of enteropathogenic and enterotoxigenic Escherichia coli in children with and without diarrhoea in Esteli, Nicaragua. J Diarrhoeal Dis Res 11:169–171[PubMed]
     [Google Scholar]
  12. Nataro J. P. 2005; Enteroaggregative Escherichia coli pathogenesis. Curr Opin Gastroenterol 21:4–8[PubMed]
     [Google Scholar]
  13. Nataro J. P., Kaper J. B. 1998; Diarrheagenic Escherichia coli. Clin Microbiol Rev 11:142–201[PubMed]
     [Google Scholar]
  14. Nataro J. P., Mai V., Johnson J., Blackwelder W. C., Heimer R., Tirrell S., Edberg S. C., Braden C. R., Morris J. G. Jr, Hirshon J. M. 2006; Diarrheagenic Escherichia coli infection in Baltimore, Maryland, and New Haven, Connecticut. Clin Infect Dis 43:402–407 [View Article][PubMed]
     [Google Scholar]
  15. Nguyen T. V., Le Van P., Le Huy C., Gia K. N., Weintraub A. 2005; Detection and characterization of diarrheagenic Escherichia coli from young children in Hanoi, Vietnam. J Clin Microbiol 43:755–760 [View Article][PubMed]
     [Google Scholar]
  16. Nweze E. I. 2009; Virulence properties of diarrheagenic E. coli and etiology of diarrhea in infants, young children and other age groups in Southeast, Nigeria.. Am Euras J Sci Res 4:173–179
     [Google Scholar]
  17. Okeke I. N. 2009; Diarrheagenic Escherichia coli in sub-Saharan Africa: status, uncertainties and necessities. J Infect Dev Ctries 3:817–842[PubMed]
     [Google Scholar]
  18. Pabst W. L., Altwegg M., Kind C., Mirjanic S., Hardegger D., Nadal D. 2003; Prevalence of enteroaggregative Escherichia coli among children with and without diarrhea in Switzerland. J Clin Microbiol 41:2289–2293 [View Article][PubMed]
     [Google Scholar]
  19. Paniagua M., Espinoza F., Ringman M., Reizenstein E., Svennerholm A. M., Hallander H. 1997; Analysis of incidence of infection with enterotoxigenic Escherichia coli in a prospective cohort study of infant diarrhea in Nicaragua. J Clin Microbiol 35:1404–1410[PubMed]
     [Google Scholar]
  20. Pereira A. L., Ferraz L. R., Silva R. S. N., Giugliano L. G. 2007; Enteroaggregative Escherichia coli virulence markers: positive association with distinct clinical characteristics and segregation into 3 enteropathogenic E. coli serogroups. J Infect Dis 195:366–374 [View Article][PubMed]
     [Google Scholar]
  21. Podewils L. J., Mintz E. D., Nataro J. P., Parashar U. D. 2004; Acute, infectious diarrhea among children in developing countries. Semin Pediatr Infect Dis 15:155–168 [View Article][PubMed]
     [Google Scholar]
  22. Regua-Mangia A. H., Gomes T. A. T., Vieira M. A. M., Andrade J. R. C., Irino K., Teixeira L. M. 2004; Frequency and characteristics of diarrhoeagenic Escherichia coli strains isolated from children with and without diarrhoea in Rio de Janeiro, Brazil. J Infect 48:161–167 [View Article][PubMed]
     [Google Scholar]
  23. Taniuchi M., Walters C. C., Gratz J., Maro A., Kumburu H., Serichantalergs O., Sethabutr O., Bodhidatta L., Kibiki G.& other authors ( 2012; Development of a multiplex polymerase chain reaction assay for diarrheagenic Escherichia coli and Shigella spp. and its evaluation on colonies, culture broths, and stool. Diagn Microbiol Infect Dis 73:121–128 [View Article][PubMed]
     [Google Scholar]
  24. Tobias J., Vutukuru S. R. 2012; Simple and rapid multiplex PCR for identification of the main human diarrheagenic Escherichia coli.. Microbiol Res 167:564–570 [View Article][PubMed]
     [Google Scholar]
  25. Vernacchio L., Vezina R. M., Mitchell A. A., Lesko S. M., Plaut A. G., Acheson D. W. 2006; Diarrhea in American infants and young children in the community setting: incidence, clinical presentation and microbiology. Pediatr Infect Dis J 25:2–7 [View Article][PubMed]
     [Google Scholar]
  26. Vilchez S., Reyes D., Paniagua M., Bucardo F., Möllby R., Weintraub A. 2009; Prevalence of diarrhoeagenic Escherichia coli in children from León, Nicaragua. J Med Microbiol 58:630–637 [View Article][PubMed]
     [Google Scholar]
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Frequency and antimicrobial resistance of diarrhoeagenic Escherichia coli from young children in Iran
J. Med. Microbiol. 63, 427 (2014); https://doi.org/10.1099/jmm.0.064600-0
/content/journal/jmm/10.1099/jmm.0.064600-0
/content/journal/jmm/10.1099/jmm.0.064600-0
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